The Antioxidant Activity of Chitosan Oligosaccharides in a D-galactose-induced Aged Mouse Model

Kong S. , Cao P.Q. , Guo J. , Su Z.Q.

Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, China

Aims: To explore the antioxidant activity of chitosan oligosaccharides (COST) in a D-galactose-induced aged mouse model.

Methods: COST was orally administered over a period of one month to C57BL/6J mice that had been previously treated with D-galactose for six weeks. The effects of COST on lipid peroxidation and antioxidant enzymatic activities were analyzed in multiple organs (liver, heart, and kidney).

Results: COST significantly inhibited the formation of MDA and enhanced the serum and tissue activities of SOD, CAT, and GSH-Px in a dose-dependent manner.

Conclusions: COST has potent antioxidant activity and may be a promising dietary supplement to slow aging and prevent age-related diseases in humans.

Acknowledgements: This project was supported by The Industry-University-Research Collaborative Innovation Major Projects of The Guangzhou Science and Technology Innovation Commission, China (No. 201604020164), and The Science and Technology Planning Project of Guangdong, China (No. 2013B021100018).

002

The Toxicity of Tripterygium Wilfordii is Weakened by Lysimachia Christinae Hance via Compataibility in Mice

Wang J.M.1,2 , Li J.H.2 , Zhang Y.Y.2 , Li J.Y.1,2 , Cai H.2 , Cui Y.1,2

1Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Chinese Medicine, Zhengzhou, China; 2College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China

Aims: To investigate the effects of attenuated toxicity of Tripterygium wilfordii(LGT) by Lysimachia christinae Hance (JQC) via compatibility in mice, and to elucidate its latent mechanism. LGT is traditionally used as a Chinese herbal medicine, and has been proven particularly effective in curing rheumatoid arthritis. However, it is associated with serious toxic side effects. According to the theory of traditional Chinese medicine (TCM), JQC may reduce the toxicity induced by LGT.

Methods: Ethyl acetate extracts (EAEs) of LGT and JQC were obtained by reflux extraction and intragastrically (ig) administered to mice once a day for 2 weeks at a dose of 60 mg/kg. Blood, in addition to hepatic and nephric tissues, were collected to assess the effects of attenuated toxicity of LGT by JQC, and to explore its preliminary mechanisms.

Results: The EAE of LGT induced an excessive increase in serum biochemical indicators such as the transferases of alanine (ALT) and aspartate (AST), creatinine (Cr), and urea nitrogen (UN), which were all significantly inhibited by the compatibility of LGT at each ratio (1/4, 1/2, 1/1, 2/1, or 4/1) to JQC. Further results indicated that the malondialdehyde (MDA) levels were lowered, however, the enzymatic activities of GST, GPx, SOD, and catalase were elevated in the mouse liver and kidneys following compatibility.

Conclusions: LGT-induced toxicity is weakened by JQC via compatibility, with a suitable component ratio from 1/4 to 4/1. The preliminary mechanism may be associated with the inhibition of oxidative stress in the liver and kidneys.

Acknowledgements: This work was supported by project grants from The National Natural Science Foundation of China (Grant No. 81503269), The Program for Science & Technology Innovation Talents in Universities of Henan Province (Grant No. 16HASTIT032), The Science and Technology Innovation Talent Fund of Henan Chinese Medicine (Grant No. 2015XCXRC01), The National Science Foundation for Postdoctoral Scientists of China (Grant No. 2012M521412), The Provincial Fundamental Research Fund in Henan University of Chinese Medicine (Grant No. 2014KYYWF-QN01), and The Doctoral Research Fund of Henan Chinese Medicine (Grant No. BSJJ2010-22).

003

Bile Salts Reduce the Chemosensitivity of Hepatocellular Carcinoma Cells by the Promotion of Survival and Proliferation via the Stat3 Signaling Pathway

Wang C.Z. , Shi X.Q. , Liao M.M., Zhao J.F. , Yang M.Y.

Key Laboratory of Nanobiological Technology of The Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China

Aims: To investigate the underlying mechanism of action of glycochenodeoxycholate acid (GCDA), a toxic component of bile salts, in hepatocarcinogenesis and chemotherapy resistance.

Methods: Cell proliferation and clonality were measured using an MTT assay and immunofluorescence, and a colony formation assay, respectively. Assessment of apoptosis was performed by flow cytometry. The mRNA and protein levels were analyzed by real-time PCR and Western blotting, respectively. shRNA was used to silence Stat3 expression.

Results: GCDA promoted proliferation of hepatocellular carcinoma cells (HepG2, QGY-7703), and reduced cell chemosensitivity following 5-FU treatment. GCDA resulted in the phosphorylation of signal transducer and activator of transcription 3 (Stat3, Y705) and the upregulation of the expression of the anti-apoptotic protein, myeloid cell leukemia 1 (Mcl-1). Following silencing of Stat3 expression, Mcl-1 expression was downregulated and the effect of 5-FU on hepatocellular carcinoma cells was reversed.

Conclusion: GCDA reduced the chemosensitivity of hepatocellular carcinoma cells, likely by the upregulation of Mcl-1 expression via the Stat3 signaling pathway.

Acknowledgements: This work was supported by The National Natural Science Foundation of China (Grant No. 81302075, 81272193 and 81402001) and The Fundamental Research Funds for Central South University (Grant No. 2017zzts391).

004

Research on the Effects of Lycorine Impact on the Tumor Cell Membrane Function

Xin G.S.1,2 , Ji Y.B.1,2

1Engineering Research Center of natural antineoplastic drugs,Ministry of Education, Harbin, China; 2Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin, China

Aims: Alkaloids are derived from a class of nitrogenous organic compounds in biology, because they mainly exist in plant, so also known as vegetable base. Alkaloids increase telomerase activity and inhibit tumor cell proliferation, reduced tumor proliferation and induced tumor cell differentiation and apoptosis, inhibiting tumor cell cycle and proliferation, and improved the body's immune function to reduce the adverse reaction of chemotherapy, and so on. Lycoris radiata alkali, a kind of important alkaloids, abounds in lycoris plants. The study found that in recent years, lycoris radiata alkali had an important role on the central nervous and cardiovascular system, had the effect of anti-inflammation and anti-virus, also had certain inhibitory effect on tumors. [Taking human liver HepG 2 cell as the research object,] We conducted an in vitroe preliminary study on antitumor activities of lycoris radiata alkali, provided theory for the research on the antitumor function of the lycoris radiata alkali basis, to the discussion of its antitumor mechanism, probe into its can be achieved through effects on tumor cell membrane structure function and inhibit the growth of tumor cells. This research will contribute to the development of lycoris radiata alkali antitumor drugs to provide new train of thought and clue.

Methods and Results: We used lycoris radiata determined by Spellotu first mention (MTT) method base on four kinds of tumor cell proliferation inhibition and selected sensitive cell lines to study the anti-tumor mechanism. We observed difference between cell apoptosis during different periods by inverted microscope. We used flow cytometry instrument to detect lycoris radiata alkali on HepG-2 cell apoptosis rate and used coomassie brilliant blue staining method, colorimetric method, total cholesterol checkerboard to detect different concentrations of lycorine We detected protein content, the change of the sialic acid content and total cholesterol levels in HepG-2 tumor cell membrane. [Show lycoris radiata alkali to the influence of chemical composition on the tumor cell membrane.] We used an ATP content determination kit to show different concentrations of lycoris radiata alkali, ? HepG-2 tumor cell membrane the influence of Na+, K+-ATPase and Ca2+, Mg2+-ATPase activity. Using the Zamudio method, we determined different concentrations of lycoris radiata alkali effect on H22 tumor cell membranes close degree. By fluorescent staining method to determine the influence of different concentrations of lycoris radiata base on cell membrane fluidity. Results found that Lycoris radiata base of four kinds of tumor cells were strong growth inhibition, and was positively related with dose dependence. Lycoris radiata alkali by inverted microscope study found that the function of human liver cancer HepG-2 cells, cells in suspension after the treatment, and as lycoris radiata alkali concentration increases, the longer the duration, the phenomenon is more obvious. Further study on the different concentration can be determined through single staining flow cytometry instrument PI lycoris radiata alkali HepG-2 cell apoptosis under the influence of the situation, The results show that when Lycoris radiata alkali concentration increased, human liver HepG-2 cell apoptosis rate also will increase, a positive correlation; L lycoris radiata alkali can significantly reduce the tumor cell membrane Na+, K+-ATPase and Ca2+, Mg2+-ATPase activity and membrane fluidity, decrease membrane protein and the content of sialic acid and close capability level, and reduce cholesterol levels. As to the increase of the dose, is negatively related to the dose.

Conclusions: Lycoris radiata alkali has antitumor activity in vitro, By reducing the tumor cell membrane on the surface of the total protein content, sialic acid content and total cholesterol levels and other chemicals, result in changes of cell membrane structure, and thus interfere with tumor cell differentiation, migration, cell recognition, adhesion and contact inhibition via a decreasing? membrane component content, resulting in the decrease of membrane fluidity, closed, enzyme activity decreased. Lycoris radiatae alkali inhibits normal tumor cell tumore cell membrane ion exchange, d destroys the cell membrane integrity and result in abnormal of tumor cell membrane function. This leads to the destruction of the tumor cells or death.

Acknowledgements: This work was supported by Harbin city technology bureau project (2016RQQXJ124); Harbin city technology bureau project (2016RAXXJ067); Heilongjiang university innovation talent project (UNPYSCT-201681).

005

Optimization of Medium Composition for Mycelial Pellet of Aspergillus Niger Y3 by Response Surface Methodology

Li L.X.1,2,# , Feng L.Y.3,# , Ma F.2 , Zhang S.4 , Zhu G.F.5 , Song Z.W.1

1School of Environment and Chemical Engineering, Heilongjiang University of Science and Technology, Harbin, China; 2State Key Lab of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China; 3School of Energy and Civil Engineering, Harbin University of Commerce, Harbin, China; 4Environmental Development Center of MEP, Beijing, China; 5Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China

Aims: During the submerged fermentation process, a kind of microbial particle could be formed by filamentous bacteria called mycelia pellet. This bio-material has high effectiveness to absorb colors and heavy metals. The greatest advantages of pellets are fast sedimentation velocity and good biocompatibility. Mycelial pellet was very effective for the immobilization of bacteria and for the degradation of target pollutants as the special carrier. The aim of this work was to obtain an efficient composition of medium for Aspergillus niger Y3.

Materials and methods: The composition of the medium was the following: sucrose 10 g/L, NH4Cl 1.0 g/L, MgSO4·7H2O 0.5 g/L and KH2PO4·3H2O 1.0 g/L. The mycelial pellet was under the cultivation condition of pH 7.0, the stirring speed of 160 r/min and the temperature of 37 °C with a production batch time of 72 h. To improve the production of biomass, the optimization of culture medium composition was carried out. Response surface methodology (RSM) was proposed in this study. Variables included sucrose, NH4Cl, MgSO4·7H2O, KH2PO4·3H2O and Box-bBehnken experimental design (BBD) model was used for data analysis.

Results: The results showed that the optimum medium composition for the culturing of mycelia pellet of Aspergillus niger Y3 was sucrose concentration 15 g/L, NH4Cl concentration 1.0 g/L, MgSO4·7H2O concentration 0.2 g/L and KH2PO4·3H2O concentration 1.0 g/L. The maximum biomass of the mycelial pellet biomass was 0.6985 g/L, and the predicted biomass of the mycelial pellet was 0.7071 g/L, under optimal condition. The condition to culture the mycelia pellet in a high quality and efficiency was created at a low price of medium and a moderate pellet size. Only 48 h were needed to form the pellet from the spores. To verify the availability and the accuracy of the models, the validation experiment was carried out. The results showed that the predicted value of the mycelial pellet biomass matched with the experimental value of the mycelial pellet biomass.

Conclusion: The efficient composition of medium for Aspergillus niger Y3 was achieved. The finding may serve as the basic data and information for the medium composition optimization of mycelial pellet.

Acknowledgements: This work was sponsored by the National Science Foundation of China (Grant No. 51408200, 51408618, 51478140, 21477122&51678222), the State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology) (Grant No. 2015DX06), the Special Fund for Science and Technology Innovation Talents of Harbin (Grant No. 2015RQQXJ015) and the Promising Youngsters Training Program of Heilongjiang University of Science and Technology (Grant No. Q20120201).

#These authors contributed equally to this work.

References::

[1]Kim J H, Lebeault J M, Reuss M. Applied Microbiology and Biotechnology, 1983, 18(1): 11–16.

[2]Prajapati S K,Kumar P,Malik A,et al. Bioenerg Res, 2014, 7(4): 1430–1440.

[3] Oncu S, Tari C, Unluturk S. Biotechnol Prog, 2007, 23(23): 836–845.

006

Polysaccharides Purified from Lycium Barbarum Protect PC12 Cells against MPP+-induced Apoptosis

Zhou Y.L.1 , Tong Y.Y.2 , Liu H.3 , Wang X.3

1School of Life Sciences, Jilin University, ChangCchun, China; 2Changchun Automobile Industry Institute, ChangCchun, China; 3The First Automobile Works Hospital, ChangcChun, China

Aims: The immunoregulatory, neuroprotective and antifatigue effects of Lycium barbarum have been widely reported. Polysaccharides obtained from L. barbarum (LBPS) have protective effects against 6-OHDA-induced PC12 cells apoptosis and prevent damage to retinal ganglion cells from acute ocular hypertension-induced ischemic injury. These encouraging conclusions let us to investigate the protective activities of LBPS on 1-methyl-4-phenylpyridine ion (MPP+)-caused PC12 cells apoptosis model.

Methods: L. barbarum purified polysaccharides entitled LBPS05 were obtained, and their structure was characterized by fourier transform infrared spectroscopy, high performance liquid chromatography, and periodate oxidation-smith degradation reaction. Four mM of MPP+ exposure was applied to establish PC12 cell apoptosis model. MTT (3-(4,5-cimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide) assay, JC-1 staining and western blot were used to detect the changes of cell viability, mitochondrial membrane potential and the phosphorylation of protein kinase B (Akt) and extracellular signal regulated kinases (ERKs) in PC12 cells.

Results: L. barbarum 05 was obtained by using a DEAE-52 cellulose anion exchange column and a Sepharose G-100 column, and was found to be a fraction with 71.5 kDa molecular weight and with the structure of (1→3), (1→4) and (1→6) linkages. LBPS05 pre-treatment effectively improved cell viability, and restored the mitochondrial dysfunction in MPP+-exposed PC12 cells. L. barbarum 05 regulated the activities of ERKs and Akt in MPP+-exposed DPC12 cells.

Conclusion: L. barbarum 05-mediated neuroprotective effects are related to the modulation of ERKs and Akt, and the subsequent shutdown of the mitochondrial apoptotic pathway.

Acknowledgements: This work was supported by the Science and Technology Key Project in Jilin Province of P.R. China (Grant No. 20160204029YY)

007

Study on the Antibacterial Constituents from Leaves of Liquidambar Formosana Hance

Xie Q.J. , Xu X.Y. , Lai X. , Wang X.L. , Zhong Y.T.

Gannan Medical University, Ganzhou, China

Aims: To study the antibacterial activity of the extracts from the leaves of Liquidambar formosana Hance in vitro, and screen its antibacterial active part.

Methods: The 70% ethanol reflux extraction from the leaves of Liquidambar formosana Hance was extracted with petroleum ether, chloroform, ethyl acetate and water saturation n-butyl alcohol respectively to get five fractions: petroleum ether extract, chloroform extract, ethyl acetate extract, n-butanol extract and water layer. The Kirby-Bauer (KB) method was applied to detect the in vitro antimicrobial activity of different solvent extracts of Liquidambar leaves to 6 strains. The trace continuous dilution method was used to detect the minimum inhibitory concentration (MIC).

Results: Different solvent extracts of Liquidambar leaves in different concentrations showed antibacterial activity to different degrees to Escherichia coli, Salmonella paratyphi B, S.flexneri, Bacillus subtilis, Staphylococcus aureusand Staphylococcus epidermidis. The water layer promoted the growth of all tested bacteria. The other extracts had different degrees of antibacterial activity, and the drug concentration was positively correlated with antibacterial activity. The antibacterial circle diameter of the chloroform extract at the concentration of 100 mg/mL on Escherichia coli, Salmonella paratyphi B, S.flexneri, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis was 6, 13, 10, 9, 10, 9 mm respectively; ethyl acetate extract was 18, 17, 17, 17, 17, 15 mm respectively; n-butanol extract 13, 16, 18, 16, 14, 14 mm respectively. The minimum inhibitory concentration of different fractions of Liquidambar leaves on different bacteria varied. The minimal inhibitory concentrations (mic) of the same extract to different bacteria was also different. The n-butanol extract had the best antibacterial effect on S.flexneri; its MIC was 0.625 mg/mL. The chloroform extract had the best antibacterial effect on Salmonella paratyphi B; its MIC was 0.625 mg/mL. The ethyl acetate extract had the best antibacterial effect on Bacillus subtilis; its MIC was 0.3125 mg/mL. The average MIC of the ethyl acetate extract was the smallest on the tested bacteria.

Conclusions: Gram-negative bacteria were more sensitive than gram-positive bacteria to leaves of Liquidambar formosana Hance. The ethyl acetate fraction showed the best antibacterial effect on the tested bacteria among the five fractions and can be used as an antibacterial active part of the leaves of Liquidambar formosana Hance.

Acknowledgements: This work was supported by a project grant from Technology Project of the Health Department of Jiangxi Province (Grand No. 20122031).

008

Yikang Decoction Facilitates Embryo Implantation in Mice with Implantation Dysfunction via the Upregulation of LIF Expression

Liu Y.H.1 , Wang J.1 , Liang S.L.1 , Huang Q.1 , Ma Z.N.1 , Chen H.L.1 , Du Y.2 , Zhang L.L.2 , Ying X.Y.1

1Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China; 2The Second Clinical Medical College, Nanjing Medical University, Nanjing, China

Aims: To explore the effects and mechanism of action of Yikang decoction on embryo implantation. Yikang decoction is a traditional Chinese medicine (TCM) that has recently been used to treat female infertility, however, the underlying mechanisms remain unclear.

Methods: Mice were randomly divided into four groups: normal, implantation failure (mifepristone treatment), Yikang decoction treatment, and control (physiological saline treatment). The efficacy of Yikang decoction was evaluated by the adhesion of uterine endometrial cells.

Results: The number of embryos was markedly reduced following mifepristone treatment. The adhesion of endometrial cells in the Yikang decoction group was significantly increased compared with those in the control group. The expression of leukemia inhibitory factor (LIF) and integrin αvβ3 was significantly reduced by treatment with mifepristone, however, the attenuated expression of LIF and αvβ3 was markedly reversed by treatment with Yikang decoction. Moreover, the expression of integrin αvβ3 was significantly decreased following LIF siRNA knockdown (< 0.01). Correlation analysis revealed a significant positive correlation between LIF and αvβ3 protein expression.

Conclusions: Yikang decoction can regulate the expression of αvβ3 and increase cell adhesion by upregulating LIF expression, thus improving embryo implantation in mice. These data provide solid experimental evidence for the efficacy of Yikang decoction in the treatment of infertility.

Acknowledgements: This work was supported by Jiangsu Province's Key Provincial Talents Program (RC2007070).

009

Scabrosones, Three Antifungal Diterpenoids from the Wild Medicinal Mushroom Earliella Scabrosa

Zhu F.1 , Li J.S.1 , Xie W.C.1 , Du Z.2

1Department of Chemical Engineering, Foshan University, Foshan, China; 2Department of Pharmacy, Foshan University, Foshan, China

Aims: To explore the chemical profile of the medicinal mushroom Earliella scabrosa and to screen for novel antifungal natural products from this medicinal mushroom.

Methods: The fruiting bodies of Earliella scabrosa were extracted with ethyl acetate. The crude extract was subjected to silica gel column chromatography, eluted with a petroleum ether-ethyl acetate gradient system, and purified by preparative HPLC. The structures were determined by MS, comprehensive NMR techniques, and X-ray crystallography. The antifungal activity was tested using a microdilution method.

Results: Three colorless oils (1-3) were obtained and elucidated as neodolastane diterpenoids (Fig. 1), and designated as scabrosone (1), monoacetyl scabrosone (2), and diacetyl scabrosone (3). Compounds 1-3 showed a potent inhibition activity against Candida albicans and Trichophyton mentagrophytes with MIC values of 100 μg/mL.

image

Fig. 1. Scabrosones (1-3) derived from the medicinal mushroom Earliella scabrosa.

Conclusions: Three antifungal neodolastane diterpenoids (1-3) were obtained from the fruiting bodies of Earliella scabrosa. These compounds may be responsible for the antifungal activity of the mushroom.

Acknowledgements: This work was supported by a project grant from the Guangdong Provincial Natural Science Foundation of China (Grant No. 2016A030313380).

010

Applying a DNA barcode to survey the authenticity of commercial Wuweizi

Ren L. , Guo M.Y. , Pang X.H.

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

Aims: Since the year 2000, the Chinese Pharmacopoeia has recorded the fruits of Schisandra chinensis and Schisandra sphenanthera as Wuweizi and Nan-Wuweizi, respectively. However, Schisandra sphenanthera is often still taken as Wuweizi either by mistake or intentionally due to its lower price and morphological similarity in the herbal market. The adulteration of the two fruits is a threat to consumer safety. Here we intended to test the authenticity of commercial products and crude drugs labeled Wuweizi using ITS2 barcode.

Methods: Wuweizi and Nan-Wuweizi samples were collected and their ITS2 barcodes were obtained to build a standard DNA barcode database. The authenticity of commercial products and crude drugs labeled Wuweizi from hospitals, herbal markets, and drug stores was subsequently tested using the database.

Results: More than 40 percent of tested commercial Wuweizi samples were authenticated as Schisandra sphenanthera.

Conclusions: The adulteration of the two fruits is a serious problem and the ITS2 barcode can be used to effectively identify Wuweizi and Nan-Wuweizi, which is a powerful supervision tool for the herbal market.

Acknowledgements: This work was supported by grants from The National Natural Science Foundation of China (Grant No. 81573541) and CAMS Innovation Fund for Medical Sciences (Grant No. 2017-I2M-1-013).

011

Research Progress in the Identification of Molecular Markers for Thyroid Carcinoma

Hou J.Z.1 , Hou Z.J.2 , Zhang J.S.1

1Department of Surgery, Cangzhou People's Hospital, Cangzhou Medical College, Cangzhou, China; 2Institute of Thyroid Disease, Cangzhou Medical College, Cangzhou, China

Aims: To study the leading research related to the identification of molecular markers for thyroid carcinoma (TC).

Methods: A literature search was carried out to analyze and integrate the papers related to the identification of molecular markers for thyroid cancer.

Results: The expression levels of Galectin-3, CK-19, p53, Livin, VEGF, Cyclin D1, β-catenin, Cyclin, CDK2, TNF-α mRNA, TNF-α, HSP70 mRNA, HSP70, and CXC chemokine receptors 4 (CXCR4) and 7 (CXCR7) were positively correlated with the degree of thyroid carcinoma. The positive expression levels of the above-mentioned proteins were significantly higher than in the adjacent and normal thyroid tissues. The positive expression levels were closely related to the degree of differentiation of TC, lymph node metastasis, depth of invasion, and AJCC stage. The expression of CXCR4 was not associated with lymph node metastasis. The absence of CD56 expression in TC tissues, combined with a high expression level of CK19 and Galectin-3, was of great significance in the differential diagnosis of TC.

Conclusion: The sensitivity and specificity of many molecular markers for TC diagnosis were not satisfactory. ROC curves were used to optimize the combination, and the ideal 3 to 4 items were selected to provide the basis for diagnosis, differential diagnosis, and prognosis of TC.

Acknowledgements: This work was supported by a project grant from: The Science and Technology Plan Project in Hebei Province (Grant No. 15277796D).

012

Electrophysiological Modeling of the Right Atrium with Thoracic Slices and Its Numerical Solution on GPU

Zhang H.1 , Liu Y.1 , Huang X.2 , Lin S.F.3

1School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, China; 2Cardiology Department, First Hospital, Xi'an Jiaotong University, Xi'an, China; 3Institute of Biomedical Engineering, National Chiao-Tung University, Hsinchu, Taiwan

Aims: Computer simulation on the action potential dynamic model is an indispensable method for the recognition of the electrical interactions between sinus node (SAN) and atrium. Creating an anatomically detailed electrophysiological model and speeding up its solution are crucial for applications of the simulation method.

Methods: Thoracic slices were obtained from the American Virtual Human Project. The anatomic model of the whole heart was picked up by Mimics. The right atrium and SAN were further isolated, and then meshed into tetrahedral elements by using the software of Hypermesh. The detailed electrophysiological model was finally developed by combining the anatomic structure with the single cellular dynamic models. The electrical excitation-conduction equation was then solved with the finite volume method by taking advantage of the operator splitting technique. In order to accelerate the solution, the Graphic Process Unit (GPU) and Compute Unified Device Architecture (CUDA) platform were used for programming.

Results: Action potentials and their conductions were consistent with the normal electrophysiological characteristics. Compared with the OpenMP program created on a computer with CPU of eight threads and four cores, the running time was reduced by more than 80% with the CUDA program.

Conclusions: The developed model and its parallel computing method in this paper established foundations for further quantitative electrophysiological simulations.

Acknowledgements: This work was supported by a project grant from the National Natural Science Foundation of China (Grand No. 81271661) and the Natural Science Foundation of Shaanxi Province (Grand No. 2016JM8069)

013

Protective Action of Naringin on Learning and Memory Deficits in Mice

Li X.B.1 , He L.1 , Li Y.J.1 , Gao X.L.1 , Zhao X.1 , Li N.2 , Wang J.M.1 , Xie Z.L.1 , Liu X.H.1 , Ding Y.W.1 , Liu L.1 , Zhang Y.T.3 , Guo H.T.2

1Henan University of Chinese Medicine, Zhengzhou, China; 2The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, China; 3Guangzhou University of Chinese Medicine, Guangzhou, China

Aims: To investigate the beneficial effects of naringin on cognitive ability related to learning and memory deficits in mice, and to elucidate its preliminary mechanism.

Methods: An acquired learning disability was established in mice with scopolamine, and subsequently naringin was administered orally for 20 days. The Morris water maze test was used to evaluate learning- and memory-related behaviors. Following the behavioral test, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), acetylcholine(ACh), and neurotrophic factor (BDNF) were measured in mouse brain tissue.

Results: Naringin decreased the escape latency time and increased the number of crossings as compared with the control group. The levels of ACh and BDNF were significantly increased in the naringin-treated group (< 0.05) as compared with the control group. Moreover, naringin increased the activity of GSH-Px and SOD, but decreased the level of MDA in mouse brain tissue.

Conclusion: Naringin can improve the learning and memory dysfunction induced by scopolamine. It is reasonable to suggest that the mechanism may be related to the promotion of BDNF expression and the increase of ACh levels in the mouse brain

Acknowledgments: This work was supported by project grants from The National Natural Science FoundatioFoundationn of China (Grant No. 81603527), The Science and Technology Project of Henan Province (Grant No. 162102310466), The Key Scientific Research Projects of Henan Province Colleges and Universities (Grant No. 16A360010), The Henan University of Traditional Chinese Medicine Scientific and Technological Innovation Talent Support Program (Grant No. 2015XCXRC05), and The General Science and Technology Project of Zhengzhou City (Grant No.20150310).

014

Studies on Quality Evaluation of Pu'er Raw Tea Based on HPLC Fingerprint

Wu N.1,2 , Xu S.J.1,2 , Liu W.1,2 , Wang Z.F.1,2 , Zhou B.1,2

1College of Science, Honghe University, Mengzi, Yunnan, China; 2Local Characteristic Resource Utilization and New Materials Key Laboratory of Universities in Yunnan, Honghe University, Mengzi, Yunnan, China

Aim: As a highly potential new ingredient for food, Pu'er raw tea from China has many health benefits and a strong cultural foundation. In China, the application of Pu'er tea has a long history. Peopople appreciate that it tastes good but no or few chemistry quality standards have been conducted. The quality of Pu'er tea in all regions does not a history of scientific evaluation that is comparable to other food items. Quality evaluation of Pu'er raw tea has already become an outstanding question. Chromatographic fingerprinting is the generally accepted technique for the assessment and quality control of honey, wine and tea. Therefore, construction of an HPLC fingerprint for the quality evaluation of Pu'er raw tea is a practical way to evaluate it. This thesis is based on 20 batches of Pu'er raw tea of varying ages, a total of 7542 samples as experimental material. We will explore the feasibility that the fingerprint technology can be used for quality evaluation and control of Pu'er raw tea.

Methods: The samples were homogenized. Then, 0.5000 g of homogenized sample was accurately weighed and was extracted with 50 mL methanol. Ultrasonic 3 KW extractors were used at 30 °C. The extracting time was 30 min. After filtration, the extracted material was transferred into a flask and condensed until near dryness by rotary evaporator with a water bath at 60 °C. Finally, the residue was redissolved with 2 mL methanol and filtered through a 0.45 μm PTFE filter for HPLC (Shimadzu) analysis. The samples were detected by an C18 column, gradient elution and UV wavelength detector (SPD-20A) in detection wavelength of 210 nm. The column was used with the mobile phase of methanol-water and a 1 μL volume of sample was injected. The total analysis time was 45 min.

Results: Under the condition of optimization, 7542 samples of Pu'er raw tea of different ages and batches were analyzed by HPLC. Their HPLC fingerprint was set up and analyzed by the traditional Chinese medicine (TCM) chromatographic fingerprint similarity evaluation system[A] 2004 edition software and the SPSS 19.0 software. The chromatographic profiles of the samples from different ages and batches of the 7542 samples of Pu'er raw tea were very similar. The indexesx of similarity were more than 0.9.The chemical constituents of Pu'er raw tea were all separated perfectly under the chromatographic condition above, and the common HPLC fingerprints of Pu'er raw tea contained eight components. The relative standard deviation of stability, repeatability and accuracy were less than 0.38%, 0.65% and 0.38%. The results show by cluster analysis that the quality in different batches of Pu'er raw tea was relatively stable and there were differences in Pu'er raw tea of the same year and different batches.

Conclusions: The experimental results show that the method is simple, rapid, stable and has a good reproducibility. The high performance liquid chromatograprhy fingerprint can objectively reflect the characteristics of Pu'er raw tea, and be used to evaluate and control the quality of Pu'er raw tea.

Acknowledgements: This work was supported by the project grant from the Department of Science and Technology of Yunnan Province (Grand No. 2012FD053 and Grand No. 2013FZ121) and the National Natural Science Foundation of China (Grand No. 61361002 and Grand No.21366011).

015

Preventive and Therapeutic Effects of Oral Lianmei on Heat and Humidity Diarrhea in Piglets

Zhu M.X.1,2 , Chen C.L.1,2# , Yang L.1,2 , Zhai S.Q.1,2

1Chongqing Academy of Animal Science, Rongchang, Chongqing, China; 2Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing, China

Aims: To explore the preventive and therapeutic effects of oral Lianmei on heat and humidity diarrhea in piglets.

Methods: Lianmei was extracted from Coptichinensis Franch. and Prunus mume Sieb. et Zucc. The berberine hydrochloride content was 23.71% and citric acid was 13.20%. A hot and humid environment was established artificially, and the piglets were fed Ecoli to induce a piglet heat and humidity diarrhea model. The therapeutic effects of oral Lianmei were evaluated by changes in antioxidant enzymes, gastrointestinal regulatory enzymes, liver ATP enzyme vitality, and the mRNA expression of duodenal apoptosis gene caspases-3, -8, and -9.

Results: The piglets treated with oral Lianmei showed a significant increase in acetylcholinesterase and amylase, and T-SOD and GSH-Px enzyme activity, a reduction in LDH leakage and MDA levels in the serum, and enhancement of Na+-K+- and Ca2+-Mg2+-ATPase vitality in the liver. Hormones were also involved in the regulation via an improvement in the thyroid growth hormone T3, and a decrease in glucocorticoid secretion. In treated piglets, the expression of genes involved in duodenal apoptosis wasere reduced; caspase-8 by 2.62 fold and caspase-3 by 5.82 fold as compared with untreated piglets. Caspase-3 reduced in a dose-dependent manner.

Conclusions: We successfully treated heat and humidity diarrhea in piglets, providing an experimental basis for clinical therapy with oral Laimei.

Acknowledgements: This work was supported by project grants from The Special Fund for Agro-Scientific Research in the Public Interest (Grant No. 201303040-06) and Chongqing Basic Research Funding (Grant No. 16423).#These authors contributed equally to this work.

016

Identification of Metastasis-Associated Gene Fusions in Salivary Adenoid Cystic Carcinoma

Lv L.Y. , Wen C. , Chen W.R. , Cheng A.Y. , Yu Y.N. , Zhang L.

Institute of Biomedical and Pharmaceutical Sciences, Hubei Key Laboratory of Industrial Microbiology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan, China

Aims: To identify the potential metastasis-associated gene fusions in salivary adenoid cystic carcinoma (SACC).

Methods: Low and high metastatic SACC cell lines were subjected to RNA profiling using paired-end sequencing. Sequencing reads were then analyzed using SOAPfuse and TopHat-Fusion algorithms. Selected fusion genes were confirmed by RT-PCR and Sanger sequencing. Bioinformatic analysis and preliminary cell biological experiments were performed to evaluate the significance of several fusion events.

Results: 24 common gene fusions were identified in low and high metastatic SACC cells. 115 and 135 fusion genes were revealed to be specifically expressed in low or high metastatic SACC cells, respectively. 20 fusion events with high span-reading numbers were confirmed by RT-PCR and Sanger sequencing. RAPGEF1-LOC100506990 fusion was found to be specifically expressed in high metastatic SACC and was further identified to be a high recurrence fusion pair in limited clinical SACC specimens, suggesting its potential application in clinical diagnosis.

Conclusion: Fusion events represent the heterogeneity of SACC, and the metastasis-associated fusion genes could be used as biomarkers for further clinical diagnosis or as therapeutic targets.

Acknowledgements: This work was supported by grants from The Natural Science Foundation of China (31501150) and The Wuhan Youth Science and Technology Plan (2016070204010140).

017

An Improved Synthetic Aperture Imaging Method Based on GPU in Medical Ultrasound

Zhang Z.H. , Liu H.F.

School of Computer Engineering and Applied Mathematics, Changsha University, Changsha, Hunan, China

Aims: In contrast to traditional ultrasound imaging methods, synthetic aperture imaging can achieve better resolution through lower operating frequencies and smaller sensor apertures. However, every generation of low resolution image needs a large amount of computation. It reduces the imaging frame rate severlyseverely. An optimized method for medical ultrasound imaging is introduced.

Methods: In order to solve this problem, an improved synthetic aperture imaging method based on Graphics Processing Unit (GPU) has been presented. The method used the theory of computational independence to parallelize the generation of image. From a macroscopic perspective, there is a computational independence between the formation of scan lines. From microscopic view, there is a certain degree of independence between the sampling points in the same scan line. Therefore, these features of synthetic aperture imaging method can take full advantage of their independent characteristics as parallel computing.

Results: In the experiment, the results show that the imaging frame rate using the new method based on GPU parallelization can effectively solve the problem of real-time synthetic aperture imaging.

Conclusions: As shown in the results, this new method based on synthetic aperture imaging and GPU parallelization can effectively improve the frame rate of imaging. And on this basis, the quality of ultrasound image will be focused in our research.

Acknowledgements: This work was supported by a project grant from the National Natural Science Foundation of China (Grand No. 61379117), and the project of Science and Technology Plan of Changsha(Grand No. ZD1601034).

018

Antidote: A WeChat Public Platform Provide Urgent Treatment for Patients with Tetrodotoxin Intoxication

Wang S.H.1,2 , Zhou X.X.1,3 , Yang J.Q3. , Nayak D.R.4 , Reyes C.5 , Dong Z.C.6 , Watson R.7 , Cattani C.8 , Young J.9 , Gray W.10 , Han L.X.11 , Yao Y.1,2 , Fang L.T.13 , Phillips P.14,15 , Du S.D.16 , Zhang Y.D.1

1School of Computer Science and Technology, Nanjing Normal University, Nanjing, Jiangsu, China; 2Department of Electrical Engineering, The City College of New York, CUNY, New York, NY, USA; 3Jiangsu Key Laboratory of 3D Printing Equipment and Manufacturing, Nanjing, Jiangsu, China; 4Department of Computer Science and Engineering, National Institute of Technology, Rourkela, India; 5John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA; 6Translational Imaging Division & MRI Unit, Columbia University and New York State Psychiatric Institute, New York, NY, USA; 7Department of Psychiatry, College of Physicians & Surgeons, Columbia University, New York, NY, USA; 8Engineering School (DEIM), University of Tuscia, Viterbo, Lazio, Italy; 9College of Natural Sciences, California State University, Chico, CA, USA; 10Department of Applied Physics, Stanford University, Stanford, CA, USA; 11School of Computing, Mathematics and Digital Technology, Manchester Metropolitan University, Manchester, UK; 11School of Information Engineering, Huadong Jiaotong University, Nanchang, Jiangxi, China; 12College of Engineering, Nanyang Technological University, Singapore, Singapore; 13West Virginia School of Osteopathic Medicine, 400 N Lee St, Lewisburg, WV, USA; 14School of Natural Sciences and Mathematics, Shepherd University, Shepherdstown, WV, USA; 15School of Electronic Science and Engineering, Nanjing University, Nanjing, Jiangsu, China

Aim: Tetrodotoxin (TTX) was discovered in fish such as pufferfish, triggerfish, ocean sunfish. It is produced by infecting bacteria in those fishes. Nevertheless, residents in China still like to consume pufferfish due to its delicious meat [1]. Therefore, pufferfish-poisoning based foodborne illnesses are reported every month, especially from March to May.

Method: WeChat is the most popular mobile messaging app for text and voice services [2]. It has more than a billion created accounts and more than 700 million active users in the world. We developed an “antidote” public platform based on WeChat. The “antidote” could guide accompanying persons to do first-aid treatment, such as inducing vomiting and artificial respiration. In addition, the “antidote” could connect the online services of nearby hospitals and try to get professional help.

Result: Twenty volunteers were enrolled to test this public platform. One volunteer said the necessary information appeared on his mobile phone just a few seconds after he inputted the keyword (such as TTX and pufferfish poisoning). Another volunteer said “antidote” warned him of the possibility of choking on pufferfish, and recommended him of preserving the food swallowed by the victim.

Conclusion: The public platform “antidote” is knowledge-intensive and prompt to help humans to respond at urgent conditions. Its greatest advantage is its ready availability. In the future, we shall try to use intelligence algorithms [3, 4] to optimize the path planning [5] to hospitals.

Acknowledgement: This work was supported by the Natural Science Foundation of China (61602250), Open Fund of Fujian Provincial Key Laboratory of Data Intensive Computing (BD201607), Program of Natural Science Research of Jiangsu Higher Education Institutions (16KJB520025, 15KJB470010), Natural Science Foundation of Jiangsu Province (BK20150983, BK20150982), Open fund of Key Laboratory of Guangxi High Schools Complex System and Computational Intelligence (2016CSCI01).

Keywords: tetrodotoxin; intoxication; food poisoning; WeChat; antidote; urgent treatment; path planning

Conflict of Interest: The authors declared this letter has no conflict of interest.

References:

1. Panao, I., et al., Puffer fish and its consumption: To eat or not to eat? Food Reviews International, 2016. 32(3): pp. 305-322

2. WeChat – Free messaging and calling app.; Available from: http://www.wechat.com/en/.

3. Ji, G.L., BBO improves tumor detection in MRI scanning. Basic & Clinical Pharmacology & Toxicology, 2015. 117(S3): pp. 19-19

4. Li, N. and Y. Shao, Conversion prediction of mild cognitive impairment to Alzheimer's disease via hybridization of two intelligence algorithms: biogeography-based optimization and particle swarm optimization. Basic & Clinical Pharmacology & Toxicology, 2016. 119(S2), Article ID: 9

5. Wu, L., UCAV path planning by fitness-scaling adaptive chaotic particle swarm optimization. Mathematical Problems in Engineering, 2013, Article ID: 705238

019

Medical Data Mining with Deep Immune Learning

Gong T. , Wang H.

College of Information Science and Technology, Engr. Research Center of Digitized Textile & Fashion Tech. for the Ministry of Education, Donghua University, Shanghai, China

Aims: To improve the acquisition of medical knowledge, with a view to advancing medical diagnosis and decision, a novel deep immune learning frame for medical data mining was proposed.

Methods: According to the statistics, the medical data had at least five characteristics: (1) diversity, (2) incompleteness, (3) privacy, (4) redundancy, and (5) time sequence. Medical data were unstructured in different formats such as signals, images, text, and videos. The incompleteness of medical record data increased uncertainty in diagnosis and decision. The medical data involved patient privacy, which was protected for safety and confidentiality. In order to discover useful medical knowledge from medical data as a whole, with a view to better medical diagnosis and decision, the medical data were preprocessed, adjusted, analyzed, mined, and learned, as shown in Fig. 1. The medical records and device data were stored in databases and preprocessed in formats for further analysis. As a novel intelligent computing mechanism inspired by the natural immune system, an algorithm was designed with deep immune learning in order to discover and learn useful knowledge from a large number of medical records, as shown in Fig. 2. All known disease data were classified into the non-self database to build the multi-dimension feature space of non-selves. The feature dimension of this space could be extended to recognize a new class of unknown non-self for unknown diseases.

Results: Unknown disease data could be learned as a new class of non-self into the feature space of non-selves. The unknown non-selves became known by deep immune learning.

Conclusions: Deep immune learning is useful for the discovery of knowledge from large datasets relating to disease and medical treatments, with a view to improving medical diagnosis and healthcare.

Acknowledgements: This work was supported by grants from National Natural Science Foundation of China (Grant No. 61673007, 61271114, and 61203325) and Innovation Program of Shanghai Municipal Education Commission (No. 14ZZ068).

image

Fig. 1. Medical data processing and analysis.

image

Fig. 2. Deep immune learning.

020

The Application of Hollow Fiber in Forensic Toxicology

Wang C.1,2 , Hao B.1,2 , Ren Y.F.1,3 , Du J.Y.1,2 , Hao H.X.1,2

1Collaborative Innovation Center of Judicial Civilization, China; 2Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing, China; 3People's Public Security University of China, Beijing, China

Aims: In forensic toxicology, in order to avoid matrix effects from the complex biological samples during the analytical workflow, the procedure before toxicological analysis is a pre-requisite. Sample preparation impacts nearly all the later assayed steps and is hence critical for unequivocal qualitative and quantitative analyses. There is a strong demand for advanced, up-to-date sample preparation technology used by hospitals and forensic toxicology laboratories for sample clean-up, isolation or concentration of target analytes from various matrices. Herein, we introduced the development and application of an emerging sample preparation method employing hollow fiber-based membrane solvent microextraction.

Methods: The fiber-based liquid phase microextraction (LPME) used in various biological samples including urine, whole blood, plasma, serum, saliva was reviewed. The extraction principle, development and application in recent years and future directions of this promising sample preparation technique are contained in this review. The paper also elaborates on the other areas such as food science, agricultural science, and environmentology.

Results: Hollow fiber-based electromembrane extraction(EME) has a huge potential in speeding up extractions, reducing the consumption of organic solvents and high preconcentration of analytes which are the evaluation index of a sample pretreatment technology. Furthermore, its high selectivity is capable of extracting various acid/alkaline drugs from complex samples.

Conclusions: Hollow fiber, as an auxiliary device of liquid phase microextraction, is economical, environmental, stable and highly effective in the chemical application of toxicology analyses. It has a great realistic significance in forensic science due to its portability and the merit which has a good combination with various analytical instruments.

Acknowledgements: This work was supported by the humanities and social science research program of China University of Politics and Law (10ZFQ82009) and Academician Foundation of the Ministry of Public Security of the People's Republic of China (No. 2011-23214203, 2011-23215243, 2011-23317015), Beijing Municipal Education Commission University Science and Technology Park Construction Project (2011-08111603) and Program for Young Innovative Research Team in China University of Political Science and Law (2014CXTD04, 16CXTD05).

021

The Effects of DSS on Expression of Caspase-3 in Cortex and Hippocampus of Rats with Chronic Cerebral Hypoperfusion

Xiao H.1,2 , Li X.2 , Liu R.Z.2

1Department of Pathology, the First Affiliated Hospital of Gannan Medical University, Ganzhou, China; 2School of Basic Medicine of Gannan Medical University, Ganzhou, China

Aims: To investigate the effects of 3′-daidzein sulfonate sodium (DSS) on the expressions of Caspase-3 in prefrontal cortex and hippocampal neurons after chronic cerebral hypoperfusion in rats.

Methods: Forty-eight Sprague-Dawley (SD) male rats were randomly divided into four groups: the sham operation group, the chronic cerebral hypoperfusion model group, the DSS group, and the positive drug control group, with 12 rats in each group. The model of chronic cerebral hypoperfusion was established by the permanent deleigation of bilateral common carotid artery. The DSS group and the positive drug control group were respectively treated with DSS at a dose of 2.0 mg/kg and 6.0 mg/kg. The sham operation group and the model group were given by an equal volume of normal saline. DSS, nimodipine or saline was intragastrically administered daily for 30 days. The damage of prefrontal cortex and hippocampal neurons was detected with the method of Nylon staining, and the expression of caspase-3 in prefrontal cortex and hippocampal neurons was detected with the western blot method.

Results: Nissl staining revealed that compared with the model group, the number of positive neurons in the prefrontal cortical and hippocampus neurons significantly decreased in DSS group (P < 0.05), and compared with the nimodipine group, there was no significant difference (P > 0.05).Western-blot detection revealed that compared with the model group, the expressions of Caspase-3 in the prefrontal cortex and hippocampal neurons significantly decreased in the DSS group (P < 0.05). Compared with the nimodipine group, there was no significant difference (P > 0.05).

Conclusion: 3′-daidzein sulfonate sodium has protective effects on the prefrontal cortex and hippocampal neurons of chronic cerebral hypoperfusion rats, and the protective mechanism may contribute to inhibiting the expression of Caspase-3 and inhibiting the neuron apoptosis of rats after the chronic cerebral hypoperfusion injury.

Acknowledgements: This work was supported by the grants from National Natural Science Foundation of China (Grant NO, 81560583) and the Natural Science Foundation of Jiangxi Province (Grant NO, 20142BAB205021).

022

Design, Synthesis and Antitumor Activity of Thiopyrano-Pyrimidine Compounds as PI3K/MTOR Dual Inhibitors

Wang Q.Q. , Zhang Z.W. , Wei N.S. , Yu Q.Y. , Xie L.X. , Zheng P.W.

Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science & Technology Normal University, Nanchang, China

Aims: BMCL-200908069-1 is a triazine-based mTOR inhibitor discovered at KuDOS Pharmaceuticals through virtual compound screening. The inhibitory activities against mTOR and PI3K kinase are 0.27 μM and greater than 10 μM, respectively. Therefore, novel derivatives of BMCL-200908069-1 still need to be synthesized and refined.

Methods: According to the principle of skeleton transition, a molecular morpholine in the BMCL-200908069-1 structure was combined with a triazine nucleus, and the oxygen atom was replaced with a sulfur atom to obtain a thiopyranosemide nucleus structure. The aromatic hydrazone structure of BMCL-200908069-1 was replaced with a pyrazole structure. Accordingly, twenty-four novel thiopyrano-pyrimidine compounds containing the pyrazole group were synthesized and the their IC50 values against the PI3K/mTOR kinases were evaluated in three cancer cell lines. The chemical structures of the compounds were confirmed by 1H NMR and MS.

Results: According to the cytotoxicity and effect on enzyme activity of the compounds, we analyzed the structure-activity relationships of the target compounds. We found that the pyrazole structure significantly increased the antitumor activity of the compounds, with compound 9 showing activities against mTOR and PI3K kinase at 3.8 nM and 66 nM, respectively. These data suggest that novel thiopyrano -pyrimidine compounds containing pyrazole structures should be synthesized, refined, and tested.

Conclusions: A series of pyrazole-containing thiopyrano-pyrimidine compounds were designed and their activities against the PI3K/mTOR kinases were evaluated. The target compounds showed excellent antitumor activity towards the PI3K/mTOR kinases. Therefore, a combination of the two active pharmacophores, the thiopyrano-pyrimidine and pyrazoline groups, resulted in the production of a novel series of PI3K/mTOR inhibitors.

Acknowledgements: This work supported by the The National Natural Science Funds of China (No. 81460527), Outstanding Youth Foundation of Jiangxi, Natural Science Foundation of Jiangxi, China (20171BCB23078), Natural Science Foundation of Jiangxi, China (20171ACB21052 & 20171BAB215073),Science and Technology Project Founded by the Education Department of Jiangxi Province (GJJ160787), Jiangxi Provincial Key Laboratory of Drug Design and Evaluation(20171BCD40015).

023

Evaluation of the Anti-microbial and Cytotoxic Activities on the Interruption of Polyketide Synthase Gene in Actinomyces Strain W062

Wang Q.F. , Wang Y.T. , Hou Y.H.

School of Marine and Technology, Harbin Institute of Technology, Shandong, China

Aims: An efficient gene engineering method has been developed for the marine actinomyces strain W062 to enhance the anti-microbial activities and cytotoxic activity.

Methods: A segment of polyketide synthase (PKS) gene was cloned and disrupted with PCR and genome walker. The anti-microbial activity was determined by observing growth inhibition of ten indicator strains. The cytotoxic activity was performed by MTT assay method.

Results: In this study, a segment of PKS gene (1128-bp) was cloned from actinomyces strain W062. This gene was disrupted to validate its function using PCR and genome walker. The results showed that W62 mutant strain enhanced the anti-microbial activities of such as E. coli, Staphylococcus aureus, Bacillus pyocyaneus, Candida albicans and Cryptococcus and cytotoxic activity against human hepatoma cell line BEL-7402.

Conclusion: Our research indicates that partial PKS gene disruption of marine actinomyces strain W62 can enhance the anti-microbial activities and cytotoxic activities, which can manipulate some compounds biosynthesis.

Acknowledgements: This work was supported by Subject Construction Fund Guided by HIT (WH20150206, WH20160206), Natural Science Foundation of Shandong Province (ZR2017MC046).

024

Study on Optimized Axiomatic Design-based Bionic Artificial Brain with Multi Anesthetic/Analeptic Factors and Least Information Quality Loss

Fan S.H.1 , Li Y.2

1MIT & NJTech Intel. United Lab of “Data Quality and Information Security”, Nanjing Tech University, Nanjing, China; 2Department of Radiology, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Nanjing, China

Aims: Design and analyze bionic artificial brains in neural-humoral environment with multi anesthetic/analeptic factors and least information quality loss. Simulate, test and optimize the designed artificial brain systems, and then apply them to some optimization fields.

Methods: Use the axiom 1 (Independent axiom) to establish a new axiom system (including a series of theorems and inference rules) which can support the bionic artificial brains. Use the axiom 2 (Information axiom) with SREA (Sexual Reproductive Evolution Algorithm) to adjust the parameters of multi anesthetic/analeptic factors to minimize the information quality loss for the total system.

Results: The bionic artificial brains designed with the optimized axiomatic design method were stable and controllable in neural-humoral environments, and they showed a high performance for all the given tasks.

Conclusions: Optimized axiomatic design-based bionic artificial brains can be applied as a “broad-spectrum” high-level solver to several optimization fields.

Acknowledgements: This work was supported by National Natural Science Foundation of China (Grant No. 71671089 and 71171110).

025

Genome-Wide Association and Network Analysis of the CSF Biomarker Tau in the ADNI Cohort

Liang H. , He B. , Liu W.J. , Chen F. , Li J.

College of Automation, Harbin Engineering University, Harbin 150001, China

Aims: To elucidate a network-based framework, guided by Protein-Protein Interaction (PPI) data, for mining high-level network-based associations with better potential for replication and biological interpretation.

Methods: SNP-level and gene-level analyses were performed on the ADNI cohort data using the Plink software and the Vegas software, respectively. Network-level analysis using the network interface miner for multigenic interactions (NIMMI) strategy was employed to identify the sub-networks. Finally, pathway analysis was used to validate the functional gene sets.

Results: The SNP-level and gene-level analyses replicated a few well-known AD genes including APOE, in addition to identifying several novel genes such as DLGAP1, DSCAM, NAV2, and PCDH9. The network-level analysis identified nine interesting sub-networks, which were replicated as the top findings in two independent analyses. Subsequent enrichment analysis indicated that the genes from these sub-networks were enriched in pathways such as neurodegenerative disease and the nervous and immune systems.

Conclusions: Our strategy detected several genes that had previously not reached significance. Moreover, this multi-level framework may provide a novel method by which to identify potential high-level candidate variants.

Acknowledgements: This work was supported by a grant from The National Key Scientific Instrument and Equipment Development Projects of China (Grant No. 2012YQ04014010) and The National Natural Science Foundation of China (61773134).

026

Application of 3VT Section in the Fetal Heart and Great Vessel Malformation in Ultrasound Examination

Tu C.Y.

Department of Ultrasound Diagnosis, Women and Children's Hospital of Linyi, Linyi City, Shandong, China

Aims: To investigate the value of the 3VT section on the examination of the fetal heart.

Methods: We conducted a prenatal cardiac ultrasound examination of the observed object and fetal echocardiography, based on the long axis view of the heart and a series of transverse section, 3VT section scanning.

Results: According to the 3VT section ultrasound image characteristics, the arterial arch axis, aortic arch axis and the short axis of the superior vena cava are from left to right. The short axis image of the upper vena cava shows the right posterior axis. The arterial arch and the aortic arch join and looked like a V-shape. Thirty-five cases of cardiac malformations were observed. The following were observed: 9 cases of pulmonary artery stenosis; 6 cases of aortic atresia; 5 cases of tetralogy of Fallot; 5 cases with persistent left superior vena cava; 3 cases of pulmonary atresia; 3 cases of persistent truncus arteriosus; 2 cases of cardiac pulmonary venous drainage; 1 case of double aortic arch and 1 case of a right aortic arch with left artery catheter and vagal left subclavian artery. In the 35 cases of cardiac malformations, the following were observed: 23 cases of the ductus arteriosus, aortic arch and superior vena cava with abnormal diameter ratio; 3 cases of reduction in the number of blood vessels; 8 cases of an increase of the number of blood vessels and 1 case of abnormal vascular branch at the confluence of the ductus arteriosus and descending aorta.

Conclusions: 3VT section was relatively easy to be obtained. In the 3VT section, with the trachea coordinate position, the number, position, diameter of blood vessels and the relationship between them can be observed and analyzed, and the judgment of the image plane can be ruled normal or not. The number of blood vessels increased or decreased; the blood vessel diameter ratio being anomalous and the vascular origin, walking, branch and tracheal showed abnormal vascular structure. The fetal heart wasere examined with 3VT. The cross section, long axis view, comprehensive observation, and comprehensive analysis are the key to improve the fetal heart and great vessel malformation diagnosis rate.

027

Clinical Application of 3D Visualization Technology in Interventional Treatment of Coronary Heart Disease

Huang Y.L.1 , Ali S.2 , Huang Q.3 , Han A.Q.1 , Liu R.Q.1

1Information Center, Beijing University of Chinese Medicine, Beijing, China; 2Department of Computer Science, University of Education, Lahore, Pakistan; 3Respiratory Medicine, Tian Tan Hospital, Capital Medical University, Beijing, China

Aims: To investigate 3D visualization technology in coronary intervention.

Methods: 120 coronary chronic total occlusion (CTO) patients were selected according to the use of a 3D visualization system for percutaneous coronary intervention (PCI). There were 60 cases in the control group. The two groups of patients were compared and analyzed with respect to the successful rate of intervention and severe complications.

Results: The successful cases in the observation and control groups were 88.5% (53/60) and 73.3% (43/60), respectively, with the difference being statistically significant (P < 0.05). The interventional complication rate in the observation and control groups was 3.3% (2/60) and 16.7% (10/60), respectively, with the difference being statistically significant (P < 0.05); and the difference in surgery duration, 75.8 + 9.1 and 100.5+15.7 min, respectively, was also statistically significant (P < 0.01).

Conclusions: 3D visualization technology used in the diagnosis and treatment of CTO intervention can significantly improve the success rate, reducing vascular dissection, coronary artery spasm, and vascular complications such as perforation. It is worthy of further promotion and clinical application.

Acknowledgements: This work was supported by a project grant from Beijing University of Chinese Medicine (Grant No. 2016-JYB-QNJSZX0005).

028

The Anti-Obesity Effect of Chitosan Oligosaccharide Capsules in a High-Fat Diet-induced Obese Rat Model

Fu C.H. , Guo J. , Su Z.Q.

Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, China

Aim: To investigate the effect of chitosan oligosaccharide capsules (COSCs) on the development of obesity.

Methods: Sprague-Dawley (SD) rats were fed either a normal or high-fat diet (HFD) with or without the anti-obesity drug, Orlistat, and high-, middle-, or low-doses of COSCs for 8 weeks. The food intake, body weight gain, fat pads, body fat ratio, and liver index were measured. Simultaneously, the serum levels of lipid and leptin were evaluated, and liver, heart, and kidney sections were observed.

Results: COSCs reduced body weight gain and fat pads, and the high body fat ratio and liver index in rats induced by a HFD, without changing the food intake. Moreover, the serum levels of TC, TG, HDL-C, LDL-C, and leptin were significantly ameliorated. The effect of COSCs was slightly superior to that of Orlistat, and it was demonstrated that COSCs were non-toxic to tissues.

Conclusions: Treatment with COSCs slowed the development of HFD-induced obesity in rats, which was most likely associated with an improvement in leptin resistance.

Acknowledgements: This project was supported by The Science and Technology Planning Project of Guangdong, China (No. 2013B021100018), The Industry-University-Research Collaborative Innovation Major Projects of The Guangzhou Science Technology Innovation Commission, China (No. 201604020164), and The National Science Foundation of China (No. 81173107).

029

Screening and Analysis of Abnormal Thyroid Function in Healthy Individuals Residing in a High Water-Iodine Area

Wang F.L. , Hou Z.J. , Mu Z.X.

Institute of Thyroid Disease, Cangzhou Medical College, Cangzhou, China

Aims: To investigate the abnormal thyroid function in healthy Cangzhou urban residents.

Method: Serum samples were collected from 3218 healthy individuals in Cangzhou city. Levels of TSH, FT3, and FT4 were determined using an immuno-chemiluminescence instrument (Roche Co., Ltd, Germany). Statistical analysis was performed.

Results: The detection rate of thyroid dysfunction increased year by year and also with age. The prevalence of hyperthyroidism, subclinical hyperthyroidism, hypothyroidism, and subclinical hypothyroidism was 0.81%, 0.68%, 1.83%, and 4.38%, respectively. The prevalence of subclinical thyroid disease (5.07%) was higher than that of clinical thyroid disease (2.64%). The incidence rate of females was higher than that of males (P < 0.001). The prevalence of hyperthyroidism was higher than that of subclinical hyperthyroidism in both males and females. The prevalence of subclinical hypothyroidism in abnormal thyroid function was the highest, accounting for 56.9%, followed by hypothyroidism and subclinical hyperthyroidism. The ratio of male to female was 1:5.4.

Conclusion: The detection rate of abnormal thyroid function in urban residents of Cangzhou increasincreaseed year by year, and subclinical hypothyroidism was the main dysfunction. Thyroid dysfunction was related to gender and age. Regular examination of thyroid function in healthy individuals will be helpful in the early and differential diagnosis of thyroid disease. Taking appropriate intervention measures will reduce the incidence of thyroid and related diseases.

Acknowledgements: This work was supported by project grants from: The Science and Technology Plan Project in Hebei Province (Grant No. 15277796D) and The Science and Technology Plan Self-Financing Project in Hebei Province (Grant No. 162777206).

030

A Robust Hybrid Algorithm Based on Windowed Fourier Filtering and Fast FCM with Phase and Magnitude Information

Mao Q. , Zhao S.G. , Gong T. , Zheng Q.Q.

College of Information Science and Technology, Donghua University, Shanghai, China

Aims: In order to overcome the sensitivity to noise and always trapped in local optimal solution problems, a novel robust hybrid approach based on windowed Fourier filtering (WFF) and improved fuzzy c-mean (FCM) algorithm (W2FCM) has been proposed in this study.

Methods: Windowed Fourier transform not only can extract the global frequency information, but also extract the local frequency information. Hence, a thoracic computed tomography (CT) image could be filtered by WFF. After filtering, the image be enhanced by incomplete beta function. As we known, phase congruency plays an important role in image processing. In this paper, the phase and magnitude information of the CT image have been extracted as initial cluster centers in FCM method.

Results: The proposed approach is more accurate than traditional methods in lung nodule segmentation. It has high noise tolerance and does not sacrifice computation time. Even more importantly, the proposed approach can get more nodule candidates for the late nodule detection. After segmentation, the shape, size and status of nodule candidates can be seen clearly. Experiments verified that this method is effective and reliable.

Conclusions: This study has proposed a novel robust W2FCM algorithm for lung nodule segmentation. This approach has noise tolerance, fast convergence and automatic segmentation advantages.

Keywords: Medical imaging; Windowed Fourier filtering; Fast fuzzy clustering algorithm; Phase and magnitude information; Lung nodule segmentation

Acknowledgements: This work was supported by the grants from the National Natural Science Foundation of China (Grant No. 61271114 and 61673007), the Fundamental Research Funds for the Central Universities (Grant No. CUSF-DH-D-2017088), Natural Science Foundation of Shanghai (Grant No. 13ZR1400200), Key Reform Project in Shanghai University Undergraduate Education (X12071306) and the Fundamental Research Funds for the Central Universities at Donghua Univ. (2232013A3-09).

031

Imaging using Optically-Enhanced Contrast Agents by Deeply Penetrating Photoacoustic Tomography

Palaniappan P. , Lee M.Y. , Park S.H. , Go S.K. , Song C.G.

Advanced Biomedical Imaging Center, Chonbuk National University, Jeonju-si, Jeonbuk, South Korea

Aims: To image the deeply-implanted optical heterogeneitity from organelles to organs by a custom-developed photoacoustic tomography (PAT) system with a linear array transducer, using optical constrast agents with a laser source to maximize the optical penetration

Methods: The functional capabilities of the PAT system were tested by imaging a phantom made from gelatin containing 10–12% water and 88–90% protein. A polymer tube with an internal and external diameter of 0.5 mm and 0.7 mm, respectively, was placed between phantom layers. Contrast agents such as indocyanine green, methylene blue, riboflavin, gold nanoparticles, silver nanoparticles, and infrared (IR-780) iodide were used.

Results: The obtained PAT images showed the feasibility and stability of the system. The laser source was tuned to a specific wavelength for each specific agent. In the future, these phantom studies can progress to animal experiments, and these contrast agents can also be loaded with tumour-targeting drugs to verify tumor location.

Conclusions: Non-invasive PAT images of contrast agents placed at various depths were obtained by the developed imaging system.

Acknowledgements: This work was supported by The Technology Innovation Program (10052749, development of an ultrafast cardiovascular diagnostic system based on multifunctional 3D ultrasound imaging) funded by The Ministry of Trade, Industry and Energy (MOTIE) of Korea, and Brain Research Program through the NRF(2017M3C7A1044819; 2017R1A4A1015681) funded by the Ministry of Science, ICT, Future Planning and Korea government(MSIP).

032

Development of a Novel Ankle Rehabilitation Robotic Platform for Hemiplegic Stroke Survivors

Liu Q.Q.1,2,3† , Duan L.H.1,7,† , Long J.J.4† , Sun T.Y.2 , Zhang X.4 , Lu Z.J.2 , Wang C.B.1,3,5 , Shi Q.3,6 , Shen Y.J.7 , Shang W.F.1 , Lin Z.H.3 , Wang Y.L.4 , Li W.G.2 , Wu Z.Z.1

1Shenzhen Institute of Geriatrics, Shenzhen, China; 2School of Mechanical & Automotive Engineering, South China University of Technology, Guangzhou, China; 3Faculty of Science and Engineering, Waseda University, Japan; 4The Second People's Hospital of Shenzhen, China; 5School of Mechanical Engineering, Guangxi University of Science and Technology, China; 6Beijing Advanced Innovation Center for Intelligent Robots and Systems, Beijing Institute of Technology, Beijing, China; 7Mechanical and Biomedical Engineering, City University of Hongkong, China; These authors contributed equally to this work.

Aims: Hemiplegic stroke survivors typically require ankle rehabilitation to recover the motor function of the ankle joint. A novel ankle rehabilitation robotic platform has been proposed to address these challenges.

Methods: The proposed rehabilitation robot consists of two symmetrical robotic platform with 3 degree of freedoms (DOFs) each. Each robotic platform employs layer-stacking structure constructed by worm gear pairs and inclined gear pairs to ensure that the rotation center of the foot pedal points to the pivot center of the ankle joint. Three ankle exercise modes, named as constant speed exercise, constant torque exercise and awareness exercise, are developed to execute ankle rehabilitation training for hemiplegic patients in the early recover stage.

Results: The preliminary experiments were performed to verify the feasibility of the robot for ankle exercise. They demonstrated that the foot pedal of the robotic platform tracked the instruction with a mean error of 1 RPM in the constant speed mode, and moved smoothly in the constant torque mode. Tthe affected side foot pedal tracked the sound side with a maximum mean error of 7.75° in the awareness exercise mode.

Conclusions: A novel ankle rehabilitation robot is developed to provide ankle exercises under constant speed mode, constant torque mode and awareness mode. Experiments with different exercise modes were executed, and the results demonstrated that the robotic platform is capable for ankle rehabilitation to hemiplegic patients.

Acknowledgements: This work was supported in part by Science and Technology Foundation of Guangdong, China (No. 2016A020220001), in part by Technology Research Foundation of Basic Project of Shenzhen, China (No. JCYJ20160428110654601 and JCYJ20160428110354308), and in part by Research Foundation of Health and Family Planning Commission of Shenzhen Municipality, China (No. 201601054), in part by the Returned Overseas Business Foundation of Shenzhen (No. 2016001).

033

Lycorine Radiata Alkali Resistance Effect and Its Mechanism of Action of Liver Cancer Research

Xin G.S.1,2 , Li X. , Xue Q.B.

1Engineering Research Center of natural antineoplastic drugs,Ministry of Education, Harbin 150076, China;;2Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China.

Aims: Lycorine widely exists in Amaryllidaceae, which is a kind of chemical monomer isolated from Amaryllidaceae. It has shown various pharmacological effects, such as the anti-tumor effect, the anti-viral, sedation and effects on the cardiovascular system. In recent years, the anti-tumor effect of lycorine was studied by domestic and foreign experts, and there are reports about the obvious inhibition effect of lycorine for human breast cancer MCF-7 cells, leukemia CEM cells and mouse melanoma B16F10 cells. But the mechanisms of its anti-tumor effect are not very clear. Usually, apoptosis can be caused through mitochondrial pathway, the death receptor pathway and endoplasmic reticulum pathway, and the mitochondrial pathway is the most classic one in apoptosis. The starting point of this thesis is the mechanism of the apoptosis of HepG-2 cells induced by lycorine through the mitochondrial pathway. The early experimental results show that lycorine promotes apoptosis on many kinds of tumor cells, such as the human liver cancer HepG-2 cells is obvious. Lycorine-induced apoptosis of HepG-2 cells was studied to provide the in-depth mechanism and thus a theoretical basis for lycorine of new anti-tumor drugs.

Method sand Results: At first this issue examines the inhibition of lycorine for HepG-2 cells growth by MTT assay. Experimental results show that lycorine had obvious effect on human liver cancer HepG-2 cells growth inhibition. With the concentrations of lycorine increasing, HepG-2 cells growth inhibition rates also increased, both with positive correlation. The IC50 was 5.73ìmol/L. Cell morphology was observed using an inverted microscope and fluorescence microscope, and measured the rate of HepG-2 cells apoptosis in different concentrations of lycorine by the flow cytometry PI staining method. Cell morphology was observed by inverted microscope in different concentrations of drugs for 48 h. The results showed that the negative control group cells grow well and closely arranged with their shape intact. In the administration group, the cell morphology had a series of changes, such as cell rounding, crushing, cell growth gradually thinning, and a large number of cells in a foloating state. The phenomenon is more obvious as the dose increases. Cell morphology was observed by fluorescence microscope in different concertrationsconcentrations of drugs for 48 h. There were apoptotic bodies, and the number of apoptotic bodies increased as the concentrations of lycorine increased. HepG-2 cells apoptosis was observed with diffferentdifferent concentrations of lycorine for 48 h by flow cytometry PI staining method. The results showed that lycorine can induce apoptosis of human liver cancer HepG-2 cells. With increasing concentrations of lycorine, apoptosis rate of human liver cancer HepG-2 cells also increased. Confocal laser scanning microscopy detected the change of mitochondrial membrane potential of HepG-2 cells after treatment by different concentrations of lycorine for 48 h. [Using kit studied Caspase-3 activity in HepG-2 cells after treated by lycorine for 48 h.] The influence of lycorine on expression of mitochondria associated protein Bcl-2、Bax、Cyt-C、Csapase-9 was analyzed by western blotting. The results showed that increasing concentrations of lycorine could reduce the mitochondrial membrane potential.. Using the microplate reader to detect Caspase-3 activity, the results showed that lycorine could increase Caspase-3 activity with positive correlation. Western Blotting analyzed different concentrations of lycorine in HepG-2 cells. The results showed that in a dose dependent relationship with the increasing lycorine, Bcl-2 protein expression was decreased, Bax protein expression increased gradually, and Bcl-2/Bax protein expression ratio decreased, and. Cyt-C protein expression was increased gradually with the increasing of lycorine concentration. At the same time, lycorine increased Caspase-9 protein expression with positive correlation.

Conclusions: The experimental results above show that lycorine can induce apoptosis of human liver cancer HepG-2 cells. The mechanism may be that, lycorine can down-regulate the expression of Bcl-2 protein and up-regulate the expression of Bax protein, the latter can promote MPTP opening and in turn decrease the mitochondria membrane potential of HepG-2 cells. The decrease of mitochondria membrane potential can cause relative high permeability in mitochondria membrane, substrate expansion of mitochondria, and rupture of outer membrane and release of Cyt-C. The release of Cyt-C in cytoplasm can activate caspase cascades reaction and activate caspase-9, finally initiating the activating of Caspase-3 and induce cell apoptosis. This showed that lycorine can induce apoptosis of HepG-2 cells through mitochondrial pathway.

Acknowledgements: This work was supported by the Harbin city technology bureau project (2016RQQXJ124); Harbin city technology bureau project (2016RAXXJ067); Heilongjiang university innovation talent project (UNPYSCT-201681).

034

Peiminine Ameliorates Diabetic Pulmonary Fibrosis

Xie Z.S.1,2 , Wang H1,2 , Liu P.1,2 , Wang J1,2 , Cui Y.1,2

1Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province; 2Pharmacy College, Henan University of Traditional Chinese Medicine

Aims: Diabetic pulmonary fibrosis (DPF) is a severe complication that increases the mortality risk of diabetes. Pulmonary fibrosis is also closely related to diabetes. But, there is no effective clinical drug treatment. Peiminine (PME) is the major alkaloid extracted from the root of fritillary (Chinese herb medicine Beimu). In this work, we investigated how PME improves DPF and the possible mechanism.

Methods: A luciferase reporter driven by a Smad (Drosophila gene ‘mothers against decapentaplegic) -containing promoter and expressed in a cell line of 293 cell to explore the PME as an inhibitor on the TGF-β/SMD pathway. mRNA or protein expression in WI-38 cells was measured by real-time PCR after high glucose or TGF-β1 incubation. Adult male Wistar rats received streptozocin (STZ, 60 mg/kg, i.p.) by intraperitoneal injection. Fasting glucose levels above 16.8 mM were considered diabetic following STZ injection 2 weeks. Diabetic rat were induced by bleomycin (4 mg/kg, b.w.) and randomized. After treatment with PME 4 weeks or not, lung tissues were isolated for mRNA, western blot or pathological analysis.

Results: In cell line levels, PME dose and time dependently inhibit the luciferase activity of TGF-β/SMAD. After WI-38 incubate with TGF-β1 or high glucose, protein and mRNA expression of α-SMA, collagen I, collagen IV, E-cadherin were increased, but PME dose and time were dependently reversed. In animal levels, PMF treatment improved the weight loss with significant difference in comparison to the DPF group in the absence of PMF. Pathological results showed that PMF alleviates pulmonary fibrosis induced by BLM. PME? decreases the expression of TGF-β1, p-Smad 2, α-SMA and collagen I in lung tissues. Moreover, the DPF rats showed significantly increased MDA, NF-κB p65 unit, TNF-α, IL-1β and decreased SOD and GSH levels in the lung tissues. Four-week treatment with PME dose-dependently reversed these inflammation and oxidative stress changes in the rats of DPF.

Conclusions: Chronic treatment with PME alleviates DPF, which is associated with TGF-β1 pathway, oxidative stress and inflammation.

Acknowledgements: This work was supported by a project grant from the distinguished talent fund of Henan University of Traditional Chinese Medicine (Grand No. 00104087) and The Key Scientific Research Project Plan of Henan Institutions of Higher Learning (Grand No. 17A360017).

035

A Management System Based on RFID Technology for Valuable Instruments in Hospitals

Liu Y. , Cheng B.H. , Zhao W.

School of Computer Engineering, Jinling Institute of Technology, Nanjing 211169, China

Aims: This paper discusses the application of RFID in the tracing, positioning, and management of valuable medical instruments, to help to improve their quality and reduce the cost of management.

Methods: We assigned a unique code to each device and wrote an RFID tag, which was associated with the background database coding information. Subsequently, RFID was monitored uninterrupted in the measured range through the RFID reader. Finally, with respect to storage, distribution, and use, the valuables management platform for real-time monitoring of expensive medical devices was established.

Results: The system can be used to monitor the location and condition of valuable medical instruments accurately and in a timely manner.

Conclusions: RFID management technology was successfully implemented in medical equipment quality control and statistical analysis to improve the quality of management of medical supplies and to help reduce management costs.

Acknowledgements: This work was sponsored by The Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 15KJB520009), The Project of the Modern Educational Technology of Jiangsu Province (No. 2017-R-54608, 2017-R-54396), The Scientific Research Foundation of the Jinling Institute of Technology (JIT-2016-jlxm-20, jit-2016-jlxm-19), and The Doctoral Scientific Research Startup Foundation of the Jinling Institute of Technology (No. JIT-B-201429, JIT-B-201411).

036

Liuwei Dihuang Decoction Improved Diabetic Cardiomyopathy by Inhibiting the Abnormal Expression of the Endoplasmic Reticulum Calcium Handling System

Cheng Y.S.1 , Chao J.2 , Dai D.Z.3 , Dai Y.3 , Zhu D.D.1 , Liu B.C.1

1Institute of Nephrology, Medical School of Southeast University, Jiangsu Sheng, China; 2Department of Physiology, Medical School of Southeast University, Jiangsu Sheng, China; 3Research Division of Pharmacology, China Pharmaceutical University, Jiangsu Sheng, China

Aims: To investigate the protective effects of Liuwei dDihuang decoction on diabetic cardiomyopathy, and to elucidate the mechanism of action.

Methods: SD rats were grouped stochastically. With the exception of the control group, animals were administered with streptozotocin by intraperitoneal injection. The treatment groups included: apocynin, aminoguanidine, and 2 doses of Liuwei dDihuang decoction.

Results: Myocardial hypertrophy and cardiac dysfunction were found in the diabetic model (DM) group as compared with the control group. The mRNA and protein expression levels of endoplasmic reticulum calcium regulatory molecules, including FKBP12.6, SERCA2a, and CASQ2, were downregulated. These were related to cardiac dysfunction and the upregulation of proinflammatory factors such as ETA, PKCε, and NADPH p22phox in the DM group. Following treatment with Liuwei dDihuang, the abnormal expression of these molecules was significantly reversed.

Conclusions: Abnormal expression of calcium regulatory molecules participated in diabetic cardiomyopathy. Liuwei dDihuang decoction improved diabetic cardiomyopathy through the upregulation of disordered calcium regulatory molecules of endoplasmic reticulum.

Acknowledgements: This work was supported by The National Natural Science Foundation of China [Grant No. 81400300] and The Natural Science Foundation of Jiangsu Province [Grant No. BK20130642].

037

Effect of Buyang Huanwu Decoction on the Expression of HPA and PCX in Diabetic Nephropathy Rats

Liu X.H.1 , Li Z.Z.2 , Ma B.X.1 , Zhao D.Y.1 , Zhao X.1 , Zhang Y.T.3 , Liu W.1 , Wang T.1 , Yang J.C.1 , Li X.B.1

1Henan University of Chinese Medicine, Zhengzhou, China; 2The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; 3Guangzhou University of Chinese Medicine, Guangzhou, China

Aims: To explore the protective effects of Buyang Huanwu Decoction (BHD) on the renal function of diabetic nephropathy (DN) rats and its preliminary mechanism.

Methods: Wistar rats were randomly divided into a control group and a DN group. Diabetic nephropathy model rats were induced by intraperitoneal injection of streptozotocin. The DN group was divided into a model group, BHD group and losartan group. At the end of experiment, blood urea nitrogen (BUN), serum creatinine (SCr), 24 h urinary protein (24-h UPr) were measured. Renal tissue was used for morphological examination by micrography. Immunohistochemistry, western blot and Real-time PCR were performed to examine the expression levels of heparanase (HPA) and podocalyxin (PCX) respectively.

Results: The results suggested that glomerular basement membrane thickness in DN rats were reduced by BHD treatment. Compared with the model group, Cr, 24-h UPr level and the expressions of HPA in the BHD group was decreased. Meanwhile, BHD could increase the PCX expression both at protein and mRNA levels.

Conclusion: Buyang Huanwu Decoction can delay the development progress of diabetic nephropathy. The mechanism is probably related to BHD inhibiting renal HPA expression, upregulating PCX expression, and maintaining glomerular charge barrier integrity.

Acknowledgments: This work was supported by a project grant from Young Core Teacher of Henan Province (2016GGJS-080).

038

Investigation on Immunomodulatory Activity of Gloeostereum insarnatum Fermentation Mycelia in Cyclophosphamide-induced Immunosuppressed Mice

Wang Y.Z.1 , Li Q.1 , Liu M.S.1 , Wang L.Y.1 , Zou S.H.1 , Wang J.T.1 , Zhou Y.L.2

1Zhuhai College of Jilin University, Jilin University, Zhuhai, China; 2School of Life Sciences, Jilin University, Changchun, China

Aims: Gloeostereum insarnatum, a popular food and biopharmaceutical fungus in Asia, displays various pharmacological activities. The present study aims to investigate the immunomodulatory activities of G. insarnatum fermentation mycelia (GIM) in cyclophosphamide (CTX)-induced immunosuppressed mice model.

Methods: Balb/c mice were intraperitoneally injected with 80 mg/kg of cyclophosphamide for three days to establish the immunosuppressed mice model. Immunosuppressed mice were orally treated with saline and 0.25, 0.5 and 1 g/kg GIM once a day for two weeks. Another 10 mice were injected with 0.16 mg/kg of Thymosin α1 twice a week for two weeks, which served as positive control group. Ten normal mice treated with saline served as the control group. After the last treatment, blood and organs were collected for further determination.

Results: Compared to non-treated immunosuppressed mice, two-week GIM treatment strongly increased organ indices, and enhanced the cytotoxicity of NK cells and the proliferation of lymphocyte. Moreover, GIM positively regulated the production of IgG and IgA, and serum levels of interleukin -2, 6, and 10. All data support that GIM possesses immunomodulatory activities in the CTX-induced immunosuppressed mouse model.

Conclusion: GIM effectively improved immune function via the modulation of interleukins in CTX-induced immunosuppressed mouse model.

Acknowledgments: This work was supported by the Science and Technology Key Project in Jilin Province of P.R. China (Grant No. 20160204029YY).

039

Synthesis and Characterization of 4-Phenylamino-6-methoxy-7-(3-thio ester) propoxy Quinazoline Derivatives

Yan H.P.1,2,3 , Shi J.G.4 , Gou G.Z.1,2,3 , Zhang J.C.1,2,3 , Xu S.J.1,2,3 , Hong Y.1,2,3

1College of Science, Honghe University, Mengzi, Yunnan, China; 2Key Laboratory of Natural Pharmaceutical & Chemical Biology of Yunnan Province, Honghe University, Mengzi, Yunnan, China; 3Local Characteristic Resource Utilization and New Materials Key Laboratory of Universities in Yunnan, Honghe University, Mengzi, Yunnan, China; 4College of Politics and International Relations, Honghe University, Yunnan, China

Aims: There is considerable interest in small molecule inhibitors of the human epidermal growth factor receptor (EGFR) family of tyrosine kinases for the treatment of cancer. 4-Amino quinazoline derivatives are significant inhibitors of the human epidermal growth factor receptor and play an key role in the treatment of cancer, saving or prolonging the life of patients. Our target is to synthesize the new 4-amino quinazoline derivatives with strong inhibition ability and high selectivity as candidate drugs.

Methods: The 4-phenylamino-6-methoxy-7-(3-thio ester) propoxy quinazoline derivatives were synthesized from N′-(5-(3-chloropropoxy)-2-cyano-4-methoxy phenyl)-N, N-dimethylformamidine by cyclization, thioetheration. The effects of temperature and protective gas on the desired product were studied in this paper.

Results: Under the condition of reaction temperature 125 °C and in nitrogen gas, the yields of the products were the higher. These structures were characterized by IR, 1H NMR, 13C NMR, MS and elemental analysis.

Conclusions: Three desired compounds, including ethyl 2-(3-(4-(3-chlorophenyl amino)-6-methoxyquinazolin-7-yloxy)propylthio)acetate, ethyl 2-(3-(4-(3-bromophen ylamino)-6-methoxyquinazolin-7-yloxy)propylthio)acetate, and ethyl 2-(3-(4-(3- chloro-4-fluorophenylamino)-6-methoxyquinazolin-7-yloxy)propylthio)acetate, were synthesized and the yields were 77.2%, 63.7% and 61.2%, respectively.

Acknowledgements: This work was supported by the projects grant from the National Natural Science Foundation of China (Grant No. 21362010) and the Open Fund of Master Pilot Construction of Chemical Subject (NO. HXY1304).

040

Antibacterial Activity Research on Litsea Cubeba Oil Produced in Gannan, Jiangxi Province

Wang X.L. , Zhong Y.T. , Huang Z.

Pathogen Biology Teaching and Research Section, Jiangxi Gannan Medical University, China

Aims: To study the minimum inhibitory concentration of Litsea cubeba oil produced in Gannan on Escherichia coli, Staphylococcus aureus, Candida albicans, Candida dubliniensis, Cryptococcus neoformans, Candida krusei.

Methods: Clinical and Laboratory Standard Institute (CLSI) broth microdilution method M27-A2 was applied to test the minimum inhibitory concentration (MIC) of Litsea cubeba oil on the six different kinds of organisms.

Results: The minimum inhibitory concentration of Litsea cubeba oil on Escherichia coli, Staphylococcus aureus, Candida albicans, Candida dubliniensis, Cryptococcus neoformans, and Candida krusei is 0.125%, 0.125%, 0.063%, 0.063%, 0.125%, 0.125% respectively.

Conclusions: Litsea cubeba oil measured in the experiment on the MIC of Escherichia coli, Staphylococcus aureus, Candida albicans, Cryptococcus neoformans, and Candida krusei are basically consistent with the reported experimental results. It should be noted that Litsea cubeba oil had a better inhibitory effect on Candida dubliniensis, and its inhibitory effect is greater than that of Candida albicans. The fact that the oil had more of an inhibitory effect on Candida dubliniensis has provided the basis and ideas for the research of lLitsea cubeba oil on the anti Candida dubliniensis.

Acknowledgements: This work was supported by a project grant from Natural Science Foundation of Jiangxi province(No: 20122BAB215023); Provincial Department of Education Youth Science Fund Project Jiangxi province,(No: GJJ 11211); Department of Health Science and Technology Project Jiangxi province (No: 20101370)

041

MRI Value in the Diagnosis of Infection Secondary to Intramedullary Dermoid Cyst with Dermal Sinus in Children

Zhang R.P.1 , Fang Y.L.2 , Zhao L.S.3 , Cai C.Q.4

1Graduate School of Tianjin Medical University, Heping District, Tianjin, China; 2Institute of Pediatric, Tianjin Children's Hospital, Beichen District, Tianjin, China; 3Department of Pathology, Tianjin Children's Hospital, Beichen District, Tianjin, China; 4Department of Surgery, Tianjin Children's Hospital, Beichen District, Tianjin, China

Aims: The purpose of this study is to evaluate magnetic resonance imaging (MRI) characteristic and its value in the diagnosis of infection secondary to intramedullary dermoid cyst with dermal sinus in children.

Methods: In this study, clinical and MRI findings were analyzed retrospectively in 7 child cases of infection secondary to intramedullary dermoid cyst with dermal sinus shown by pathology. An MRI scan was performed in all cases before operation, 5 of which were performed with enhanced MRI.

Results: Among 7 cases, 5 cases occurred in the lumbar region, 1 case in the thoracic region, and 1 case in the thoracolumbosacral region. An MRI scan of the spine revealed that the intramedullary dermoid cyst in 6 cases were round-like or oval-like, parallel to the vertical axis of the spinal cord, and the cyst in 1 case was string of beads-like. And the MRI also showed the cyst in 6 cases were well-defined and the cyst in 1 case was ill-defined. Additionally, the dermoid cyst in 7 cases showed hypo-intense on T1-weighted imaging (T1WI) and hyper-intense on T2-weighted imaging (T2WI). Among them, 6 cases showed homogeneous signal, 1 case showed heterogeneous. Iso-intense septation on T1WI was seen in 2 cases. As for dermal sinus in this study, MRI showed iso- or hypo-intense both on T1WI and T2WI. Enhanced MRI showed no enhancement in 1 case, slight enhancement in cyst wall of 4 cases, and enhancement in the sinus of 2 cases.

Conclusions: Magnetic resonance imaging has important value in diagnosis of infection secondary to intramedullary dermoid cyst with dermal sinus, which can display the location, contour, and signal intensity of intramedullary dermoid cyst and dermal sinus.

Acknowledgments: This work was supported by the project grant from the Key Project of Tianjin Health Care Professionals (Grant no. 16KG166) and the National Natural Science Foundation of China (Grant no. 81771589).

042

Bionic Hierarchical Artificial System of Brain, Cerebellum and Medulla in Humoral Environment with Multi Anesthetic/Analeptic Factors

Fan S.1,2 , Catherine A.3

1MIT & NJTech Intel. United Lab of “Data Quality and Information Security”, Nanjing Tech University; 2Wolfson College, University of Cambridge, Cambridge, UK; 3US & Canada Headquarters, CS Corporation, New York, USA

Aims: Design and analyze a bionic integrated artificial system of the nervous systems in a humoral environment. Simulate and test the collaboration mechanism of the hierarchical system. Optimize the integrated model and apply it to other fields.

Methods: We used the artificial self-organizing Hopfield neural network for the brain, the artificial cerebellum model for the cerebellum and the ordinary BP neural network for the medulla. We built the humoral environment to integrate the whole hierarchical neural network system which act as the collaboration intermediary. We adjusted the humoral parameters with multi anesthetic/analeptic factors and checked the stability and the controllability of the model.

Results: The bionic hierarchical model was stable and more controllable in humoral environments, and it showed a high performance for all the tasks.

Conclusions: With appropriate humoral environments, this bionic hierarchical artificial system can be more competitive.

Acknowledgements: This work was supported by National Natural Science Foundation of China (Grant No. 71671089 and 71171110).

043

A Rise of Kaempferol Content In Ethanolic Extract From Saffron Stigmata (Crocus sativus L.) Reduces Chaperone Proteins Polyubiquitination in Rat Hepatic Cells Injured by Ischemia-Reperfusion

Bazylak G.1 , Wu T.-H2,3 , Wang P.-W4 , Pan T.-L.4 , Leu Y.-L.5 , Wu Y.-C.2

1Department of Pharmaco-Bromatology & Molecular Nutrition, Faculty of Pharmacy, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland; 2Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan; 3Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; 4School of Traditional Chinese Medicine, College of Medicine, Chang-Gung University, Taoyuan, Taiwan; 5Graduate Institute of Natural Products, College of Medicine, Chang-Gung University, Taoyuan, Taiwan

Aims: Preferential bioaccumulation of free kaempferol and variety of its glycosides have been observed in various aerial parts of Crocus sativus L. [1]. Thus, functional proteomic tools were used to evaluate the protective effect of the increased to 20% (w/w) kaempferol content in ethanolic extract (KAREX) obtained from saffron, dry stigmata from C. sativus L., which strictly complied the specified origin status and quality criteria of the European Pharmacopoeia in a rat animal model upon hepatic ischemia-reperfusion (IR) injury.

Methods: Apoptotic damage to hepatocytes in three groups of male rats: 1) sham, 2) IR, and 3) IR pretreated with KAREX was evaluated by histologic analyses. Redox proteomics assay with the 2-D gel electrophoresis oxyblot and MALDI-MS were applied to clarify the protein carbonylation caused by reactive oxygen species (ROS) related with IR. In addition, the preventive effect of KAREX on the hepatic IR injury in rats was revealed with flow cytometry and immunohistochemistry experiments.

Results: Significantly decreased carbonylation of specific chaperone proteins, functionally involved in the pro-apoptotic processes, was significantly abolished by KAREX. The five proteins as COMT, SMP30, GRP75, GRP78 and valosin containing protein (VCP) were restored in rat hepatic cells under KAREX pretreatment and this result confirmed data reported earlier by Pan et al. [2]. However, in current studies, some additional not previously reported 10 proteins as RATGSTB, CA-III, beta-actin, APTase-beta chain, HSP60B, catalase, TKT, transferrin, carbamoyl-phosphate synthase and HSC73 were also observed in KAREX treated rats.

Conclusion: These results indicated that the kaempferol enriched KAREX effectively attenuated the hepatic cell death process and protein oxidation which could lead to develop a novel therapeutic approaches against ROS related malignances and diseases.

Acknowledgements: This work was supported by National Science Research Grant of Taiwan (NSC-98-230-B-182-017), Chang Gung Memorial Hospital, Taoyuan, Taiwan (CMRPD170192) and Nicolaus Copernicus University, Bydgoszcz, Poland (PDS-407-2016/2017).

References:

[1] Bazylak G, Pan TL, Wang PW, Leu YL, Wu YC, Wu TH, Acta Biochim Pol 2011; 58(Suppl. 3): 6-8.

[2] Pan TL,Wu TH, Wang PW,Leu YL, Sintupisut N,Huang CH,Chang FR, Wu YC, Proteomics 2013; 13:2297-2311.

044

Automatic Detection of the Coronary Artery Wall in Intravascular Ultrasound Images Using a Level Set Model

Lee J.H.1 , Hwang Y.N.2 , Kim G.Y.2 , Jiang Y.Y.2 , Kim S.M.1,2

1Deparment of Medical Devices Industry, Dongguk University-Seoul, Seoul, Korea; 2Departmemt of Medical Biotechnology, Dongguk University-Bio Medi Campus, Goyang-si, Gyeonggi-do, Korea

Aims: This study presents an automatic detection approach based on a level set model for segmentation of the coronary artery wall in sequential intravascular ultrasound (IVUS) images.

Methods: Sequential IVUS image frames were acquired from human coronary arteries using 20 MHz and 45 MHz catheters. The initial estimation of the vessel wall borders was done manually only for the first frame. After the initialization, pre-processing, including edge preservation, noise reduction, and dead zone preservation, was successively performed on each IVUS frame. To improve the segmentation performance, the image masks of each boundary were determined preliminarily by local binary pattern-based mask initialization. Then, the vessel borders were approximated using a modified distance regularized level set evolution model. The reliability and reproducibility of the manual segmentation were validated in terms of inter- and intra-observer agreement. Evaluation parameters, including area, perimeter, and diameter, were computed from the segmented regions to quantify the detection accuracy.

Results: The results indicated superior performance of the proposed method for detecting coronary artery wall from the IVUS images. The proposed method was strongly correlated with the manual segmentation for all evaluation parameters with a high degree of statistical significance (r > 0.716, P < 0.05). In addition, the proposed method was suitable for the three-dimensional vessel reconstructions.

Conclusions: These experimental results suggest that the proposed method has clinical applicability for the diagnosis of cardiovascular diseases based on IVUS image.

Acknowledgements: This work was supported by the International Collaborative R&D Program funded by the Ministry of Knowledge Economy (MKE), Korea (N01150049, Developing high frequency bandwidth [40–60 MHz] high resolution image system and probe technology for diagnosing cardiovascular lesion).

045

Construction of a Long-acting Antibacterial Surface on Implanted Titanium Plate

Huang X.1 , Xu Y.J.2 , Yang L.C.2 , Wu J.Q.2 , Zheng X.1 , Fei J.3,4

1School of Sports and Health Science, Tongren University, Tongren, China; 2College of Material and Chemical Engineering, Tongren University, Tongren, China; 3Center of Trauma of PLA, Daping Hospital, Third Military Medical University, Chongqing, China; 4State Key Laboratory of Trauma, Burn and Combined Injury, Third Military Medical University, Chongqing, China

Aims: In order to increase the drug load capacity and to prolong drug release time, a streptomycin sulfate (SS) loaded polymer film was prepared on the surface of titanium plate (TP), which may realize long-acting anti-infection.

Methods: Streptomycin sulfate was encapsulated into maleic anhydride grafted polylactic acid (MPLA) nanoparticles, which were spread evenly on the amino modified surface of TP. Then polyethylenimine (PEI) was filmed on the nanoparticles. Based on the electrostatic attraction of COO- on MPLA nanoparticles and NH+ on PEI, the Layer-by-Layer self-assembly (LBL) technique was used to construct SS loaded multilayer films. Prepared TP was placed into a dialysis membrane immersing into PBS with continuous stirring to determine the SS release behavior in vitro.

Results: The results of atomic force microscopy and water contact angle measurement suggest that MPLA/PEI multilayer film is successfully synthesized on the surface of TP. The cumulative SS release only reached 68.47% after 120 h with 7 layers of MPLA nanoparticles in the film.

Conclusions: The LBL film on TP had the good property of sustained drug release which worked in long-acting antibiosis. It could be inferred that such modified TP would have great potential in implantation of titanium plates.

Acknowledgements: This work was supported by Starting Up Foundation for PhD of Tongren University (trxyDH1518), Key Grant of Guizhou Education Committee (KY2015399), Science and Technology Cooperation Project of Guizhou Province (LH20157237), Science and Technology Fund of Guizhou Province (20161152), Medical Research Funding of PLA of China (AWS14C003), Special Funds for Social Undertaking and Livelihood Security Projects of Chongqing (CSTC2016SHMSZX130068) and Youth Development Program of Medical Technology of PLA (16QNP103).

046

Umbilical Cord Mesenchymal Stem Cells as a Promising Treatment for Acute Disseminated Myelitis that is Unresponsive to Classical Medication

Dong H.J.1 , Li G.1 , Luo Y.C.2 , Yin H.J.3 , Wang H.3 , Liu W.C.3 , Zhao M.L.4 , Lin L.1

1State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin, China; 2State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China; 3The Biomedical Engineering Institute of the Chinese Academy of Medical Ssciences Tianjin, China; 4Tianjin Key Laboratory of Neurotrauma Restoration; Neurological Hospital, Affiliated Hospital of Logistics University of the Chinese People's Armed Police Forces, Tianjin, China

Background: Stem cell therapy is another medical revolution following drugs and surgery. Mesenchymal stem cells (MSCs) possess significant self-renewal and multilineage differentiation properties, and can be harvested from the bone marrow, adipose tissue, skin, umbilical cord blood, umbilical cord, and peripheral blood, as well as other organs 【1-5】. Here, we report a case of a 63-year-old female with acute disseminated myelitis whose disease was not sensitive to classical medication, but was sensitive to umbilical cord mesenchymal stem cell (UC-MSC) treatment. The patient recovered well 2 months post-UC-MSC transplant.

Aims and Methods: To observe the therapeutic effects of UC-MSC on acute disseminated myelitis. We recruited a 63-year-old female whose disease was not sensitive to classical medication. The patient received a UC-MSC transplant 4 times (1 x 109). We subsequently extracted the patient's cerebrospinal fluid (CSF) and evaluated the differential proteins using the sequential windowed acquisition of all theoretical fragment ions technique.

Results: The patient made an encouraging recovery following UC-MSC transplant. We identified a total of 813 proteins in the patient's CSF, 116 of which were differentially-expressed between pre- and post-treatment samples, including tropomyosin receptor kinase C (TrkC, post:pre-treatment >3), neurotrophic factor-3, which promotes regeneration of the nervous system (NT-3, post:pre-treatment = 3.79), hepatocyte growth factors (HGFs), matrix metalloproteinases (MMPs), and heat shock protein 70 (HSP70). The functions of such proteins suggest that the pathway is involved in evading apoptosis and promoting angiogenesis.

Conclusions: UC-MSC transplant may be a promising treatment for acute disseminated myelitis patients whose disease is not sensitive to classical medication. The 116 differentially-expressed proteins between pre- and post-treatment samples may be promising novel therapeutic targets.

Acknowledgements: This work was supported by a project grant from The National Natural Science Foundation of China (Grant No. 81401295), The Tianjin Research Program of Application Foundation and Advanced Technology (Grant No. 15JCQNJC45200 and 16JCYBJC27600), The PUMC Youth Fund and The Fundamental Research Funds for the Central Universities (Grant No. 3332015126).

References:

1. M.F. Pittenger, A.M. Mackay, S.C. Beck, R.K. Jaiswal, R. Douglas, J.D. Mosca, M.A. Moorman, D.W. Simonetti, S. Craig, D.R. Marshak, Multilineage potential of adult human mesenchymal stem cells, Science 1999; 284: 143-147.

2. Liu CB, Huang H, Sun P, Ma SZ, Liu AH, Xue J, Fu JH, Liang YQ, Liu B, Wu DY, Lu SH and Zhang XZ. Human Umbilical Cord-Derived Mesenchymal Stromal Cells Improve Left Ventricular Function, Perfusion, and Remodeling in a Porcine Model of Chronic Myocardial Ischemia. Stem Cells Transl Med 2016; 5: 1004-1013.

3. Cai M, Shen R, Song L, Lu M, Wang J, Zhao S, Tang Y, Meng X, Li Z and He ZX. Bone Marrow Mesenchymal Stem Cells (BM-MSCs) Improve Heart Function in Swine Myocardial Infarction Model through Paracrine Effects. Sci Rep 2016; 6: 28250.

4. Dong HJ, Shang CZ, Li G, Niu Q, Luo YC, Yang Y, et al. The Distribution of Transplanted Umbilical Cord Mesenchymal Stem Cells in Large Blood Vessel of Rats With Traumatic Brain Injury. J Craniofac Surg 2017; 28: 1615-1619.

5. Kassis I, Vaknin-Dembinsky A and Karussis D. Bone marrow mesenchymal stem cells: agents of immunomodulation and neuroprotection. Curr Stem Cell Res Ther 2011; 6: 63-68.

047

Influence of Polygonum Cuspidatum Anthraquinone and Panax Notoginseng Saponins on MCP-1/TNF-a Levels in Diabetic Nephropathy RATS Kidney

Wang H.1,2 , Wang J.2 , Liu P2 , Dong B2 , Liu G2 , Zhao Z.B.2 , Miao M.S.2

1Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Zhengzhou, China; 2Pharmacy College, Henan University of Traditional Chinese Medicine, Zhengzhou, China

Aim: To investigating the influence of Polygonum cuspidatum anthraquinones and Panax notoginseng saponins (PNS) jointly on the renal monocyte chemotactic protein- 1(MCP-1)/tumor necrosis factor-alpha (TNF-a) in early diabetic nephropathy (DN) rats and to explore the possible mechanisms of them, which can provide the experimental basis for clinical application of DN.

Methods: We used an intraperitoneal injection of streptozotocin (STZ) (65 mg/kg) to induce the Sprague-Dawley (SD) rat model, with fasting blood glucose equal to or larger than 16.7 mmol/L as the successful model of the experimental basis. SD rats were randomly divided into 7 groups: normal group; model group; Polygonum cuspidatum anthraquinone group; PNS group; Polygonum cuspidatum anthraquinone/PNS (1:1) group; Polygonum cuspidatum anthraquinone/PNS (1.5:1) group; Polygonum cuspidatum anthraquinone/PNS (1:1.5) group,and 10 rats in each group. Normal group and model group rats per 100 g body weight were administrated with normal saline 1 mL. Polygonum cuspidatum anthraquinone/PNS (1:1, 1.5:1, 1:1.5) group rats were given respectively for anthraquinone of Polygonum cuspidatum anthraquinone (8.75 mg/kg, 13.13 mg/kg, 8.75 mg/kg) and PNS (35 mg/kg, 35 mg/kg, 52.5 mg/ Kg), daily irrigation stomach administered once, medicine for 8 weeks. The expression of MCP-1 and TNF-a in rat kidney was determined by immunohistochemistry, and the morphological changes of the kidney were observed by optical microscope. The strength of the immunohistochemical reaction can be shown by Integral Optical Density (IOD) that was used to represent the intensity of the immunohistochemical reaction.

Results: Compared with the model group, the expression of MCP-1 in each drug group was decreased. The1:1.5group was the most decreased, the expression of TNF-a in each group was also decreased, and the effect of the combined group was better than that of the single use group.

Conclusion: Polygonum cuspidatum anthraquinones and PNS had obvious inhibitory effect on MCP-1/TNF-a expression in rat kidney, thus slowing kidney injury in DN rats, and delaying the process of DN.

Acknowledgements: This work was supported by a project grant from the distinguished talent fund of Henan University of Traditional Chinese Medicine (Grant No. 00104087).

048

The Application of New Media Propaganda Model in the Management of a Primary Hospital

Wu L.Y. , Wang X.Y. , Xu J.L. , Xu H.W.

People's Hospital of Tongcheng, Xianning, Hubei, China

Aims: Through the investigation and making contrastive analysis of the understanding degree of local residents’ comprehension on hospital profile, knowledge popularization activities and preferential policies before and after the application of the new media propaganda model in a Second Grade Hospitals, the paper aimed at discussing the roles of new media in hospital propaganda. In order to develop and utilize the new media in the hospital publicity, the paper provided some reference to formulate the propaganda model that suits the management and development of the grass-roots hospital, thus achieve the maximum effectiveness of publicity.

Method: After reading the relevant literature, self-designing and improving the questionnaire after consulting the relevant experts, the authors made two surveys on the local residents of a Second Grade Hospitals in Xianning City during two periods, March to April of 2015 and March to April of 2016. A total of 2000 questionnaires were distributed and 1806 of them were collected as valid ones after removing the invalid ones. So the effective rate was 90.3%. The data that did not adopt optimized new media propaganda model before March of 2015 was set as the control group and the data that used the optimized propaganda model from 2015 April to 2016 April was set as the observation group. Based on the feedback from the residents in the survey results in 2015 and the problems in the application process, the paper puts forward a series of reform measures, including richening the content of official website, Weibo and We-Chat, reasonably controlling frequency of pushing messages to ensure the timeliness of the information, enhance the use of the publicity function of the new media platform, establishing a systematic and scientific hospital publicity management system. The paper selected the hospital's basic information, activities, activity policies, new media platform and other comprehensive information to compare the differences of the understanding degree between the two groups. The paper adopted SPSS18.0 for the database analysis and collected the original data for comparison between the groups. A chi-square test was adopted and P < 0.05 for the difference was statistically significant.

Result: Control group: The total score of the residents’ understanding degree on the comprehensive information of the hospital was 36.91%. Among it, the score for the understanding degree on the basic information of the hospital was 46.87%, the score for the understanding degree on the hospital activity and policy was 31.66%, and the score for the understanding degree on the hospital new media platform was 28.08%. Observe group: the residents’ scores in understanding the hospital comprehensive information, the basic information package, activities and policies, the new media platform in hospital was respectively, 57.31%, 63.05%, 53.73% and 55.15% (all of P < 0.05). These results indicated that after optimizing the publicity model, the understanding degree of residents on hospital information has been significantly improved and the differentiation wasere significant between them. As a result, the optimization of the new media publicity model in the hospital publicity and management has a positive effect.

Conclusion: Through the optimization and application of the new media publicity and management model, the public's understanding degree on the hospital's basic information, theme activities, fees, preferential policies and new media advertising platform and other comprehensive information has great improvement, this means the optimized hospital publicity model is of certain value to the popularization and application in primary hospitals.

Key words: new media, hospital publicity and management, primary hospital, hospital publicity, public understanding

049

Improving Emotion Regulation Ability by Real-time fMRI Connectivity-based Neurofeedback

He W.J.1 , Bu H.B.1 , Qin Y.L.1 , Gao H.1 , Tong L.1 , Li Z.L.2 , Yan B.1

1China National Digital Switching System Engineering and Technological Research Center, Zhengzhou, Henan, China; 2Department of Radiology, People's Hospital of Zhengzhou University, Zhengzhou, Henan, China

Aims: In view of the human emotional research, most mental functions relate to functional interactions within the emotion network. As the real-time functional MRI (fMRI), neurofeedback (rt-fMRI NF) can play an important role in the emotion regulation, learning direct control over functional connectivity might provide a novel approach improving emotion regulation ability.

Methods: We showed for the first time that participants could learn to change functional connectivity within the emotion network by controlling the functional connectivity between the left amygdala and the right superior medial gyrus, which are involved in emotion processing. An Region of interest (ROI)-based feedback group whose regulation target was the activation of the left amygdala, as well as sham-feedback group and none-feedback group in our experiment was set as a comparison.

Results: After training, participants in the connectivity-based feedback group successfully self-regulated the target connectivity, and showed more significant enhancement in amygdala-prefrontal connectivity than other groups.

Conclusions: Our results demonstrate that connectivity-based neurofeedback training enhances emotion regulation capacity, and facilitates the greater modulation of emotion network. The connectivity-based approach can contribute to therapeutic emotion regulation protocols for neuropsychiatric disorders.

Acknowledgements: This research was supported by the National Key Research and Development Plan of China under grant 2017YFB1002502, the Henan Natural Science Foundation of China (No.U160411981).

050

Genome-Wide Promoter Dynamics of DNA Methylation in Tissue-Specific Changes during Development

Cai Y.N.1 , Yang R.H.1,2

1College of Information Science and Technology, Jinan University, Guangdong Sheng, China; 2Meizhou Science and Technology Bureau, Meizhou, China

Aims: During the development of the mammalian genome, DNA methylation changes frequently. These changes are important to gene expression and certain other processes. Elucidating how and to what extent CpG islands (CGIs) are methylated during cell development is essential to understanding the function of DNA methylation and its relationship to gene expression.

Methods: Changes in DNA methylation at 13,296 autosomal promoters in the liver tissue from C57BL/6J mice at three stages of development (newborn, NB; embryo aged 15 days, E15; adult aged 12 weeks, AD) were analyzed.

Results: Changes in DNA methylation are not stable but instead dynamic. During the development process, both demethylation and de novo DNA methylation took place. Among the CpG-poor promoters, we found no obvious relationship between methylation and gene activity. However, with respect to de novomethylation, there appeared to be preferential targets. In comparison, the majority of promoters in strong CpG islands were unmethylated. There was a negative correlation between the DNA methylation status and the HCP activities. Moreover, some short motifs were overrepresented in the methylated regions, suggesting that they may play a role in the targeting process.

Conclusions: Our study shows that the methylation states of promoters can be predicted by their sequences and gene function during tissue development.

Acknowledgements: This work was supported by project grants from The NSF project of China (No. 61302131, 61104206), The International S & T Cooperation Program of China (ISTCP) (No. 2015DFI12970), The Guangdong Science and Technology Program (No. 2015B010105012, 2014B050505011, 2013B010136002, 2015B020214004, 2014A050503046, 2015B020233010, 2016B020211002), The Guangzhou Science and Technology Program (No. 201508020083), and The Guangxi Science and Technology Program (No. 2015AA08210).

051

Research Advances in the Identification of Gene Markers for Thyroid Carcinoma

Hou Z.J.1 , Hou J.Z.2

1Institute of Thyroid Disease, Cangzhou Medical College, Cangzhou, China; 2Department of Surgery, Cangzhou People's Hospital, Cangzhou Medical College, Cangzhou, China

Aims: To summarize the research progress in the identification of thyroid cancer-related genes in the diagnosis and differential diagnosis of thyroid cancer.

Methods: A literature review was carried out to collect and analyze papers related to thyroid cancer genes.

Results: Ras gene mutations often occur in the early stages of thyroid carcinoma (TC). The H-ras gene mutation rate was related to the degree of differentiation. The N2-ras mutation was the most predominant in follicular thyroid carcinoma (FTC), and more than 90% of papillary thyroid carcinoma (PTC) had the rearrangement, with RET/PTC1 also being relatively common. The mutation rate of the p53 gene was closely related to PTC differentiation, lymph node metastasis, depth of invasion, and AJCC stage. The detection rate of BRAF gene mutations in PTC was very high, and was related to postoperative recurrence. The expression of Bcl-2 and Survivin was closely related to the histological type, pathological grade, clinical stage, and lymph node metastasis of TC.

Conclusion: Co-detection of thyroid cancer-related genes and the use of ROC curves to elucidate the optimal combination of 3 to 4 ideal genetic markers are of great value in the diagnosis and prognosis of benign and malignant thyroid nodules.

Acknowledgements: This work was supported by a project grant from: The Science and Technology Plan Project in Hebei Province (Grant No. 15277796D).

052

Optimized Synthetic Routes of Osmertinib

Zhao B.B. , Xiao Z. , Zhang H. , Zhou Y.Z. , Zheng P.W.

Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science & Technology Normal University, Nanchang, China

Aims: Osimertinib, a epidermal growth factor receptor (EGFR) inhibitor, is mainly used to the treatment of non-small cell lung cancer (NSCLC) patients with specific epidermal growth factor receptor sensitivity (EGFRm+) T790M resistance mutations and inefficacy after other EGFR blockers treatment. Compared with previous reported EGFR inhibitors gefitinib and afatinib, osimertinib showed excellent activity against H1975 cell line, with the IC50 values at the nanomolar level. What's more, osimertinib also has lower cytotoxicity and side effects. In this research, we analyzed and summarized the existing synthetic method of osimertinib, and found the best industrial production route for the synthesis of osimertinib.

Methods: We analysed all reported synthetic routes of osimertinib through comparing raw material costs, yield, post-processing method, by-product species, and the degree of risk of the reaction operation, and determined the suitable commercial production way of synthesizing osimertinib. The structures of all compounds were determined by 400 MHz spectrometer (1H NMR) and mass spectra (MS).

Results: By analyzing the results of the various routes, we discovered a suitable industrial production way for synthesizing osimertinib. Taking indole as starting material, the intermediate (1) 3-(2-chloropyrimidin-4- yl) -1- methyl -1H-indole was synthesized by the reactions of methylation and nucleophilic substitution. Next, using 4-fluoro-2-methoxy-5-nitroaniline as the starting material, the intermediate (2) N-(5-amino-2-((2-(dimethylamino) ethyl) (methyl)amino)-4-methoxyphenyl)acrylamide was afford throughout Boc protection, substitution, reduction, acylation and deprotection to get. Finally, intermediate 1 and intermediate 2 were reacted with each other under basic conditions to obtain the final compound osimertinib.

Conclusion: In summary, N-(2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxy -5-((4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)acrylamide was synthesized from the commercially obtained indole and 4-fluoro-2-methoxy -5-nitroaniline. The optimized synthetic route of osimertinib is feasible, higher yield, more environmentally friendly and suitable for industrial production.

Acknowledgements: This work was supported by The National Natural Science Funds of China (No. 21662014), Outstanding Youth Foundation of Jiangxi, Natural Science Foundation of Jiangxi, China (20171BCB23078), Natural Science Foundation of Jiangxi, China (20171ACB21052 & 20171BAB215073),Science and Technology Project Founded by the Education Department of Jiangxi Province (GJJ160787), Jiangxi Provincial Key Laboratory of Drug Design and Evaluation (20171BCD40015).

053

Dynamic Changes in H3k9me3 and H3k9acin in Bovine Preimplantation IVF Embryos

Ma X. , Zhang M.L. , Ma P.P. , Yang S.B. , Luan W.M.

College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China

Aims: Dynamic changes in histone H3 lysine 9 tri-methylation (H3K9me3) and histone H3 lysine 9 acetylation (H3K9ac) were examined in bovine oocytes and preimplantation IVF (in vitro fertilization) embryos in order to understand their physiological role.

Methods: To determine the dynamic changes in H3K9me3 and H3K9ac in bovine preimplantation embryos and oocytes, IVF preimplantation embryos were collected at different stages of development and immuno-stained for H3K9me3 and H3K9ac.

Results: H3K9me3 and H3K9ac were distributed in the nucleus of oocytes and embryos, and have a similar trend in dynamic change. H3K9me3 and H3K9ac were expressed in GV stage oocytes but not in MII oocytes. Strong H3K9me3 and H3K9ac signals were observed at the 2-cell and 4-cell stage and were maintained during IVF early embryo development, however were not seen at the 8-cell stage. Nevertheless, notably, almost no signals were found in 8-cell IVF embryos.

Conclusion: H3K9me3 and H3K9ac signals were maintained in bovine IVF early embryo development, with the exception of at the 8-cell stage. These results suggest that H3K9me3 and H3K9ac may play essential roles in bovine IVF early embryo development.

Acknowledgements: This work was supported by grants from The National Natural Science Foundation (No. 31302047) in China and The Natural Science Foundation of Jilin province (No. 20170101018JC).

054

Recognition of Muscle Transverse Section in Ultrasound Image Based on Gray Co-occurrence Matrix

Luan K.1 , Wang H.Y.1 , Li J.1 , Liu J.L.2

1College of Automation, Harbin Engineering University, Harbin, Heilongjiang, China; 2Neurological Rehabilitation Department, Heilongjiang Province Rehabilitation Hospital, Harbin, Heilongjiang, China

Aims: Stroke is the third leading cause of death in the world. Spasticity is one of the disorders that often occur in the aftermath of a stroke. The intramuscular application of botulinum toxin type A (BTX-A) has emerged to be an established treatment to reduce muscular hyperactivity due to spasticity. Ultrasound image guided BTX-A injection has been applied widely for muscular spasm treatment. The key of this method is to extract the transverse section in ultrasound images and allow the needle into the center point. However, it is difficult to localize a muscle in muscle groups due to the low image quality of the ultrasound.

Methods: This paper presents a novel method to recognize the transverse section of a muscle in muscle groups in ultrasound images. First, a changing area is produced by the passive movement of the muscle while concerned limb is being pulled. Second, three areas: severe, slowly and no changing are selected from the ultradsound images. The energy of gray co-occurrence matrix obtained from the three area are is compared. Final, a model is built to represent the intensity of image changing.

Results: The method was verified by two images sequences obtained from two volunteers. Energy is closely related to the intensity of image changing. 2D ultrasound images showing transverse sections of target muscles were recognized by the proposed model.

Conclusions: The results suggested that the energy of gray co-occurrence matrix could identify the changing area in ultrasound images. The proposed method be applied to a muscle in muscle groups localization in ultrasound image.

Acknowledgements: This work was supported by the National Natural Science Foundation of China (Grant No. 81301297), the Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20132304120032), Applied Technology Research and the Development Foundation of Heilongjiang Province (Grant No. GC13A308), the Applied Technology Research and the Development Foundation of Harbin City (No. 2016RAQXJ043).

055

Research on Medical Vehicle Location and Navigation Technology in Intelligent Transportation

Yao J.D.1,2 , Yan X.Y.2 , Wang C.S.2

1School of Network and Communication, Jinling Institute of Technology, Nanjing, China; 2Nanjing Intelligent Transportation Innovation Center, Nanjing, China

Aims: To apply wireless location technology to medical vehicle positioning and navigation in intelligent transportation.

Methods: We evaluated the advantages and disadvantages of various range-based and range-free algorithms for use in location estimation technology, and elucidated the main factors that affect the estimation of the location of medical vehicles. Subsequently, we analyzed the accuracy of the wireless method and its applicability in the evaluation of medical vehicle location.

Results: We preprocessed the medical vehicle communication data in a medical vehicle environment, and analyzed the sensitivity of the distance propagation model parameters to the localization error of the fusion algorithm.

Conclusions: The fusion algorithm based precisely on two positioning methods, range-based and range-free, was better than that using only one location technique.

Acknowledgements: This work was sponsored by a grant from The Science and Technology Development Program of Nanjing (No. 2016sb331024) and The Nanjing Special Funding Project in 2016 for Software and Information Services Development.

056

Study of the Laminar Low Shear Stress-induced Proteomic Discrepancy in the Endothelial Cells

Zhao Y.W.1 , Wang L.1 , Ren P.L.1 , Wang S.C.1 , Li J.1 , Dai H.Y.1 , Wu J.2 , Zhao X.3 , Nie Y.M.1

1Collage of Basic Medicine Institute, Xinjiang Medical University, Urumqi, China; 2College of Basic and Forensic Medicine, Sichuan University, Chengdu, China; 3Department of Physics, East China Normal University, Shanghai, China

Aims: Shear stress not only influences the endothelial cell morphology, migration, differentiation and proliferation, but also regulates the expression of proteins in the endothelial cells. In the present study, we have examined the low shear stress-induced proteomic discrepancy in the endothelial cells to better understand the mechanism of shear stress on vascular endothelial cells.

Methods: Human umbilical vein endothelial cell was cultured on glass slides and placed in a parallel plate flow chamber. M199 culture medium was used for low laminar shear stress at 4.58 dyn/cm2, 2 h for the testing group, and the rested controls. Electrophoresis technique was used for the analysis. Mass spectrometer was used to analyze the proteins at the different spots. NSBInr, Swissprot, DAVID, database was applied for the analysis of the molecular function, biological process and other information.

Results: Ten of the special expressed protein spots were chosen from control (n = 8) and test (n = 2) groups. Compared with the controls, there were 14 proteins from the tested group were increased and 78 were down regulated.

Conclusions: Combined proteomic and bioinformatic approach, this study was successfully identified some biomarker in HUVECs and might provide new insights into the mechanism of atherosclerosis progression.

Acknowledgements: This work was supported by a project grant from the National Natural Science Foundation of China (Grand No. 11462022).

057

Processing by Lysimachia Christinae Hance Attenuates Tripterygium Wilfordii-Induced Toxicity in Vivo

Wang J.M.1,2 , Li J.Y.2 , Cai H.2 , Li J.H.1,2 , Zhang Y.Y.2 , Chen R.X.2 , Cui Y.1,2

1Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Chinese Medicine, Zhengzhou, China; 2College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China

Aims: To explore the mechanisms of action underlying the lowered toxicity of Tripterygium wilfordii Hook (TW) via processing by Lysimachia christinae Hance (Pro-LC). TW is a medicinal plant that has been widely used in China for centuries and has been proven to be effective in curing rheumatoid arthritis (RA). However, this treatment results in severe toxic side effects. The Traditional Chinese Medicine theory suggests that LC may reduce the toxicity caused by TW.

Methods: The processed products of TW were prepared by boiling in water together with the LC extract until all liquid had evaporated. Raw and processed TW products were used to prepare ethyl acetate refluxing extractions (EAREs). Each EARE was administered intragastrically to mice for 2 weeks at a dose of 60 mg/kg. Serum biochemical markers were used to evaluate the anti-toxicity action, and hepatic and nephric tissues were examined to evaluate its preliminary mechanisms.

Results: The TW raw material led to a significant elevation in serum ALT, AST, Cr, and UN levels, and tissue TNF-α and MDA, and a significant decrease in SOD, CAT, and IL-10 in hepatic and nephric tissues. The above abnormal indicators were all significantly reversed by Pro-LC. Moreover, pathological observation indicated that the TW raw material led to obvious hepatic and nephric tissue injury, however, no significant damage was detected in tissues from the mice treated with the processed product of TW by Pro-LC.

Conclusions: Pro-LC could attenuate TW-induced toxicity, with a potential mechanism of preventing oxidative stress damage in vivo.

Acknowledgements: This work was supported by project grants from The National Natural Science Foundation of China (Grant No. 81503269), The Program for Science & Technology Innovation Talents in Universities of Henan Province (Grant No. 16HASTIT032), The Science and Technology Innovation Talent Fund of Henan Chinese Medicine (Grant No. 2015XCXRC01), The National Science Foundation for Postdoctoral Scientists of China (Grant No. 2012M521412), The Provincial Fundamental Research Fund in Henan University of Chinese Medicine (Grant No. 2014KYYWF-QN01), and The Doctoral Research Fund of Henan Chinese Medicine (Grant No. BSJJ2010-22).

058

Development of An Arm Robot with Human-like Motor Nerve Model for Neurological Examination Training

Wang C.B.1,2,3,4,5,* , Liu Q.Q.1,2,3,* , Duan L.H.1,4 , Sun T.Y.2 , Shi Q.3 , Shen Y.J.4 , Shang W.F.1,6 , Li W.G.2 , Wu Z.Z.1 , Zecca M.3,7 , Wei J.J.5 , Takanishi A.3

1Shenzhen Institute of Geriatrics, Shenzhen, China; 2School of Mechanical & Automotive Engineering, South China University of Technology, China; 3Faculty of Science and Engineering, Waseda University, Japan; 4Mechanical and Biomedical Engineering, City University of Hongkong, China; 5Guangxi University of Science and Technology, China; 6Faculty of Mechanical Engineering, Chinese University of Hongkong, China; 7Loughborough University, Loughborough, Leicestershire, United Kingdom; *These authors contributed equally to this work.

Aims: Neurologic examination is an important component of the physical examination. Traditional methods are used to help trainees to improve their skills or experiences. However, a more lively training method is needed to improve the training effectiveness. In this paper, an arm robot with motor nerve model is proposed.

Methods: This arm robot provides a more realistic simulation of motor nerve disorders linked to their pathology. A robust simulation of human arm and motor nerve structure made it possible to reproduce various symptoms related to the examination of elbow force, biceps tendon reflex, and involuntary action. A humanlike motor nerve model with multi-parameters is embedded in the robot. During the simulation, motor nerve model mimics the signal generation and transformation of human brain and motor nerve pathway; the control system of human like arm drives the arm to generate the symptoms basing on the changes of the signal.

Results: The proposed robotic arm simulator is instrumental for neurologic examination training, and the proposed model can be expanded to other applications of simulating human motor nerve abilities

Acknowledgements: This work was supported by project grants from No. 2016A020220001, No. 2016001, No. 201601054, No. JCYJ20160428110654601 and JCYJ20160428110 354308, JCYJ20170413095245139, JCYJ20170306170851910, No. GJHZ20170331 105318685.

059

The Synthesis of Clopidogrel Intermediates

Huang Y.J. , Zhu Q.X.

Yichun Vocational Technology College, Yichun, China

Aims: A number of clinical experiments have shown that clopidogrel intermediates have good efficacy, safety, and tolerability. The synthetic route is described here.

Methods: Chlorobenzyl cyanide was combined with three bottles of apis (500 mL) and heated to 100 °C for 3-3.5 h. 1 mL methylene chloride was mixed with 1.3 mL water and stirred for 10 min. The solution was left to cool for 10–15 min, and 0.6 mL sodium bisulfite was subsequently added and stirred for 10–10 min, and left to cool for a further 10–15 min to separate the lower organic layer. Next, 0.6 mL methylene chloride was used for extraction, merging the organic layer. Washing steps were carried out with 0.6 L saturated sodium bicarbonate and 0.65 L saturated brine, and dried with anhydrous magnesium sulfate for 1–2 h, enriching to a dry oily matter, which was mixed with 1.2 mL n-hexane, cooled, stirred thoroughly, and filtered. A light yellow crystal resulted, which was dissolved in 4.3 mL anhydrous methanol, and sodium bicarbonate and pyridine hydrochloride were then added for phase transfer catalyst. After feeding, the system was heated to reflux for approximately 5 h, and then cooled to 0 °C with mixing for 1.5–2 h. Washing steps were carried out using 3 mL water 2–3 times, and then 1.4 mL cold methanol. After being dried, the reaction was heated for 5 h, to yield a white/yellow granular crystalline powder. Potassium hydroxide was dissolved in 3 mL water and cooled to 10 °C, then clopidogrel isopropyl alcohol was added for phase transfer catalyst. The reaction was heated to reflux while stirring for more than 8 h, and then cooled to room temperature. 6N hydrochloric acid was added to pH to 6–7 and mixed to crystallization for 2–3 h at 0-5 °C. The reaction was filtered with 2.5 mL water after fully drying, cooled to 0-5 °C, mixed, filtered, and dried in a vacuum at 0.08–0.1 MPa, 40-45 °C. The reaction was baked for 1 h at 60 °C and then for 8–12 h to yield a white/yellow granular crystal.

Results: The preparation methods of clopidogrel were reviewed and clopidogrel was finally synthesized from 2-chlorobenzyl cyanide via bromination, condensation, hydrolyzation, esterification, and resolution. Finally, the sulfate was re-crystallized as the target compound clopidogrel.

060

Detection of Hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX) Using an Electrochemiluminescence Immunosensor

Ren Y.F.1,2 , Hao B.2,3 , Zhou H.4 , Bai F.B.2,3 , Hao H.X.2,3 , Yang R.Q.1

1People's Public Security University of China, Beijing, China; 2Collaborative Innovation Center of Judicial Civilization, China; 3Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing, China; 4Institute of Forensic Science, Ministry of Public Security, Beijing, China

Aims: Currently, terrorist bombings occur all over the world. In China, the selective and efficient detection of hazardous explosives such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and cyclotetramethylenete-tranitramine (HMX) are increasingly important for public safety. Therefore, nano materials and an electrochemiluminescence immunoassay (ECLI) were adopted as sensing technology for the detection of RDX and HMX, experimentally.

Methods: It has been reported that the luminol-functionalized silver/graphene oxide composite (luminol/AgNPs/GO) and N-(aminobutyl)-N-(ethylisoluminol)/hemin dual-functionalized graphene hybrids (A-H-GNs) indicated excellent ECL activity. Herein, a label-free electrochemiluminescence immunosensor for RDX detection was developed. Firstly, positively-charged chitosan-coated A-H-GNs were introduced into the reservoir of an indium-doped tin oxide electrode by drying at room temperature. Secondly, negatively-charged luminol/AgNPs/GO modified with a monoclonal antibody against RDX were dropped onto the surface of the electrode for 6 h at 4 °C, which was designed to form an anti-biotin-SA-luminol-AgNPs-GO/CS-A-H-GNs/ITO electrode (i.e., an immunosensor) through simple electrostatic adsorption interactions. This sensing platform could offer an excellent ECL signal interface with the recognition site.

Results: When RDX is present, a remarkable decrease in the ECL signal is observed as a result of the formation of an RDX-antibody complex. RDX can be detected according to the differences between the previous and current signals. It was found that the limit of detection was reaching ppt level.

Conclusion: The immunosensor can be proposed for the detection of explosives with a high sensitivity. Our group will analyze the selectivity and stability of the sensor, and apply the technology to practical cases of explosives detection in water and soil.

Acknowledgements: This work was supported by the Fundamental Research Funds for the Central Universities (No. 2016JKF01210).

061

Data Mining for Local Liver Hydatid Disease in Xinjiang Based on a BP Neural Network

Pazilya Y.1 , Yan C.B.2 , Murat H.2 , Yao J.3 , Zhang S.X.2

1College of Basic Medicine, Xinjiang Medical University, Urumqi, China; 2College of Medical Engineering Technology, Xinjiang Medical University, Urumqi, China; 3Department of Radiology, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, China

Aims: To discuss the application of data mining technique in CT image classification of local liver hydatid disease in Xinjiang.

Methods: The textural features of CT images of normal liver and two kinds of hepatic hydatidosis were extracted using both the db4 wavelet transformation and the gray-level co-occurrence matrix (GLCM), and the optimal texture feature subsets were selected using a statistical method. The BP neural network algorithm was used to construct a classifier with which to classify the selected subset of features. The final classification model was used in the subject's work characteristic (ROC) curve analysis and parameter evaluation.

Results: CT images of 100 normal livers, 100 single cyst type of hepatic echinococcosis and 100 hepatic cysts were classified. The results showed that the accuracy rate of db4 wavelet classification was 96.33%, with the accuracy rate of the gray level co-occurrence matrix being 88.67%. The accuracy rate of the comprehensive feature, consisting of the db4 wavelet feature and the grayscale symbiosis matrix, reached 98.33%.

Conclusions: The BP neural network classifier had a high classification ability for the comprehensive texture features of Xinjiang local liver hydatidosis CT images extracted using both the gray-level co-occurrence matrix and the db4 wavelet transformation, which provides a basis for the diagnosis of hepatic hydatid disease. This data also laid the foundation for the research and development of a computer- aided diagnosis system for liver hydatid disease in Xinjiang.

Acknowledgements: This work was supported by The National Natural Science Fund (Grand No. 81460281, 81560294, 81160182).

062

The Protective Effect of Sasanquasaponin on Myocardial Ischemia-Reperfusion Injury in Rats

Xiao H.1,2 , Li X.2 , Chen Y. P.2 , Chen L.Y.2 , Zeng R.Y.2

1Department of Pathology, the First Affiliated Hospital of Gannan Medical University, Ganzhou, China; 2School of Basic Medicine of Gannan Medical University, Ganzhou, China

Aims: To explore the protective effect of sasanquasaponin (SQS) on myocardial ischemia-reperfusion injury in Rats.

Methods: A total of 30 male Sprague-Dawley (SD) rats were divided into three groups: sham- operated group, myocardial ischemia-reperfusion injury model group and SQS group, with ten rats in each group. The myocardial ischemia-reperfusion injury model was induced by ligaturing the left anterior descending artery. The model group and the SQS group were ligated for 30 min and then released for 180 min. The left ventricle coronary artery of the sham- operated group was only exposed, not ligatured. Ten min before ligaturing, the SQS group was injected with SQS 1 mg/kg through the sublingual vein of rats. The sham-operated group and model group were injected with equal volume normal saline. After administration, the cardiac hemodynamic changes were recorded with the physiological recorder, and the morphological changes of myocardial tissues were observed by Hematoxylin and Eosin (HE) stain.

Results: Compared with the model group, the heart rate (HR), the left ventricle systolic pressure (LVSP) and the maximum of decrease rate of left ventricular pressure (-dp/dtmax) value of the SQS group increased significantly (P < 0.05), and the maximum of increase rate of left ventricular pressure (+dp/dtmax) value of the SQS group increased significantly (P < 0.01). The myocardial fiber of the sham-operated group was arranged in order, without degeneration, necrosis and inflammatory cell infiltration in myocardial interstitium. The myocardial fiber of model group was presented with disorder arrangement, congestion and myocardial interstitial edema. The inflammatory cell infiltration was increased obviously and myocardial necrosis was occurred. In the SQS group, the disorder condition of myocardial fiber arrangement was improved,edema was relieved, and the inflammatory cell infiltration and myocardial necrosis in myocardial tissue were significantly reduced .

Conclusion: Sasanquasaponin has a protective effect on myocardial ischemia-reperfusion injury in rats, and the effect may be associated with the improvement of cardiac hemodynamic function and myocardial tissue structure.

063

Establishment and Efficiency of Mycelial Pellet-microalgae Coupled System for Treatment of Domestic Sewage

Li L.X.1,2,# , Feng L.Y.3,# , Ma F.2 , Song Z.W.1 , Liu Y.4

1School of Environment and Chemical Engineering, Heilongjiang University of Science and Technology, Harbin, China; 2State Key Lab of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China; 3School of Energy and Civil Engineering, Harbin University of Commerce, Harbin, China; 4College of life science and technology, Harbin Normal University, Harbin, China

Aims: Bioaugmentation was one of popular directions in the field of wastewater treatment research and played an important role in the field of improving water quality and system operational stability. The technology enhanced the removal efficiency to target pollutants and relied on biological carriers to maintain the concentration and activity of algaes, especially regarding nitrogen and phosphorus removal in wastewater. Development of mycelial pellet-microalgae coupled technology has certain guidance functions to the treatment technologies of domestic sewage. The preparation conditions and efficiency of mycelial pellet-microalgae coupled system for treatment of domestic sewage were studied. Mycelial pellet-microalgae coupled system was named as the fungus-algae pellet.

Materials and Methods: The spore suspension of Aspergillus niger Y3 was inoculated in the domestic sewage with inoculation amount 20 mL/L. The age of spore was 20-26 day, the culturing temperature was 37 °C, the initial culturing pH was 7.0, shaking speed was 160 r/min, and mycelial pellet was formed after 2 day. The mycelial pellets and algaes (Scenedesmus obliquus) were inoculated in the domestic sewage at the same time. The fungus-algae pellets were formed after 6 h with 30 °C, 160 r/min, 3000 lux. Efficiency of fungus-algae pellets for treatment of domestic sewage: In order to investigate its removal effect on ammonia nitrogen and COD of domestic sewage, the fungus-algae pellets were applied in the flask. Comparisons of the performances were conducted between two flasks inoculated with mycelial pellets and algaes respectively.

Results and conclusion: The removal efficiency of ammonia nitrogen with fungus-algae pellets was 78%, significantly higher than the removal efficiency of the control with mycelial pellets which was 50% and the removal efficiency of the control of algaes which was 61%. The removal efficiency of COD with fungus-algae pellets was 69%, much higher than the removal efficiency of the control with mycelial pellets which was 37% and The removal efficiency of algaes which was 45%. It was proven that mycelial pellets as biomass carrier was feasible. The effectively immobilized microalgae, stability maintaining of microalgae in the system, and target pollutants degradation with high efficiency were achieved.

Acknowledgements: This work was supported by the National Science Foundation of China (Grant No. 51408200, 51478140&51678222), the State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology)(Grant No. 2015DX06), the Special Fund for Science and Technology Innovation Talents of Harbin(Grant No. 2015RQQXJ015) and the Promising Youngsters Training Program of Heilongjiang University of Science and Technology (Grant No. Q20120201).

References:

[1] Zhang S, Li A, Cui D, et al. Biological improvement on combined mycelial pellet for aniline treatment by tourmaline in SBR process. Bioresource Technology, 2011; 102:9282-9285.

[2] Zhang S, Li A, Cui D, et al. Performance of enhanced biological SBR process for aniline treatment by mycelial pellet as biomass carrier. Bioresource Technology; 2011, 102: 4360–4365.

#These authors contributed equally to this work.

064

Study on Temperature Elevation of Photoacoustic Tomographic Coupling Medium to Guide Intensity Variation in Photothermal Therapy

Palaniappan P. , Lee M.Y. , Go S.K. , Park S.H. , Song C.G.

Advanced Biomedical Imaging Center, Chonbuk National University, Jeonju-si, Jeonbuk, South Korea

Aims: Coupling medium for photoacoustic tomography (PAT) plays a vital role in the acquired strength of the acoustic signal. The purpose of this study was to investigate temperature elevation of the coupling medium during guidance to acquire the maximum intensity in photothermal therapy (PTT).

Methods: We used 10% IR 780 iodide loaded-poly(lactic-co-glycolic acid)-poly(ethylene glycol) (PLGA-PEG) micelles as the imaging sample. The water temperature of the custom-developed PAT system was varied from 25 °C to 37 °C in a specialized water tank. The speed of sound when varying the temperature was accounted for. The PTT images (Therm-App) were acquired using a continuous laser with 1 W/cm2 power.

Results: The intensity variations in PAT images were mapped using the developed algorithm. The PAT signal rises as the temperature of the water increases. Prior to conducting the PTT in a specimen, the increased PAT signal was used to identify the location of the tumour.

Conclusions: The improved PAT signal as a result of increasing the temperature of the coupling medium effectively located the tumor in the specimen, enabling maximum intensity during PTT.

Acknowledgements: This work was supported by The Technology Innovation Program (10052749, development of an ultrafast cardiovascular diagnostic system based on multifunctional 3D ultrasound imaging) funded by The Ministry of Trade, Industry and Energy (MOTIE) of Korea, and Brain Research Program through the NRF(2017M3C7A1044819; 2017R1A4A1015681) funded by the Ministry of Science, ICT, Future Planning and Korea government(MSIP).

065

Research on the Effects of Lycorine Impact on the Tumor Cell Membrane Function of Tumor-bearing Mice

Ji Y.B.1,2 , Xin G.S.1,2

1Engineering Research Center of natural antineoplastic drugs, Ministry of Education, Harbin, China; 2Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin, China

Aims: Lycorine are rich in Amaryllidaceae plants, which contain a variety of unique biological and pharmacological activity, such as anti-tumor, anti-bacterial, anti-virus and other pharmacological effects. Lycorine can serve protection to the cardiovascular system, central nervous system. As a natural ingredient found in nature, lycorine has a rich content, and it has a wide range of pharmacological activity. In recent years, its anti-tumor effect has received attention among research scholars. As an important component of tumor cells, cell membranes play a vital role in the maintenance and regulating of the physiological characteristics of tumor cells. If cell membranes change, the tumor cells will shift and proliferate. If a drug working on tumor cells plays a role, it must pass through cell membranes first, and in the process, changes in membrane function will change with biochemical reaction membrane. Therefore, in order to show the influence of H22 cells membrane biochemistry and structure of the material induced by lycorine, we verify how derivatives of [lycorine can affect the cell membrane structure, changes in membrane structure and affect its function channels, thereby inducing cell death, to achieve the anti-tumor effect. This would, provide a theoretical basis for the anti-tumor mechanism of lycorine.

Method sand Results: Our group intends to study 10 mg/kg, 20 mg/kg, 40 mg/kg lycorine working on H22 tumor cell membranes to have an impact. Lycorine would cause the membrane to produce changes in physiological activity, and lead to an anti-tumor. We used methods in vitro to measure the impact to the immune organs of tumor-bearing mice after they were given lycorine. We detected how different concentrations of lycorine influence the chemical composition protein content of the cell membrane of H22 tumor cells by coomassie brilliant blue staining and how different concentrations of lycorine influence the chemical composition of sialic acid content of the cell membrane of H22 tumor cells by colorimetric. We used a total cholesterol test kit to detect different concentrations of lycorine to influence the chemical composition cholesterol content of the cell membrane of H22 tumor cells. An ATP determination kit detected that different concentrations of lycorine influence [Na+, K+-ATPase and Ca2+, Mg2+-ATPase] of the H22 tumor cell membrane. And determine the effect of different concentrations Lycorine of membrane fluidity by fluorescence staining; determine the effects of different concentrations of H22 Lycorine blocking degree of tumor cell membrane by Zamudio method.] Experimental results show that lycorine has obvious effects on the immune organs by [measuring the impact of immune organs. ]Lycorine can reduce the total protein content, SA content and cholesterol levels of the cell membrane of H22 tumor cells, with increasing dose, and dose-related negative state. Lycorine is able to reduce tumor cell membrane fluidity, membrane blocking degree, and can reduce the Na+/ K+-ATPase and Ca2+/ Mg2+-ATPase activity, with increasing dose, and dose-related negative state.

Conclusions: In summary, Lycorine can reduce the total protein, sialic acid and cholesterol on the cell membrane of tumor cells, thereby undermining its original physical structure and reducing its membrane fluidity. Lycorine can also reduce Na+, K+-ATPase and Ca2+, Mg2+-ATPase as well as the closure of the ATPase activity of tumor cells. tumor outside the influence of the material transport membrane, inhibition of metastasis and differentiation of tumor cells, [tumor cells reduces the ability to maintain a stable,] in the result is tumor cells of normal physiological function disorder, and eventually leads to tumor cell death. It is suggested that lycorine can influence cell function leading to tumor cell H22 disintegration or death by acting on the cell membrane structure .

Acknowledgements: This work was supported by the Harbin city technology bureau project (2016RQQXJ124); Harbin city technology bureau project (2016RAXXJ067); Heilongjiang university innovation talent project (UNPYSCT-201681).

066

Basic Research to Develop a Korean Pronunciation Self-Study System for Chinese Learners Using Formant Comparison and Bio-Signal Measurement

Song H. J.1 , Wei Q.2

1Tabula Rasa College, Keimyung University, Daegu, Korea; 2Department of Biomedical Engineering, School of Medicine, Keimyung University, Daegu, Korea

Aims: Use a formant comparison and measuring bio-signal to find pronunciation difficulty factors for Chinese Korean language learners during speaking processing.

Methods: For the experiments, a four-channel bio-signal measurement system was used to acquire the bio-signal. Ten Chinese subjects who were studying Korean in middle level were invited to take part in this experiment. Each subject was required to wear a noise-cancelling headset to listen to each question and was required to answer in Korean. The Electrocardiograph (EEG), Galvanic Skin Response (GSR) and Photoplethysmography (PPG) sensors were attached to the subject for measuring the bio-signal. With a developed analysis algorithm, the peak amplitude of the recorded Chinese learner's voice was measured on the time domain, and the corresponding formant was obtained on the frequency spectrum.

Results: The formant comparison algorithm is able to show analytic reasons of the pronunciation method for the learners’ errors. Combined with the results of processed bio-signals, the algorithm can give effectively customized tips for correcting pronunciation problems.

Conclusions: This study has shown that the concept of using the formant comparison and bio-signal can be applied to the Korean pronunciation self-study system for Chinese learners to learn Korean sounds accurately.

Acknowledgements: This research was supported by the Keimyung University Research Grant of 2015.

067

Isolation, Identification, and Antibacterial Activity of Two Steroids Secreted by the Stored Products Pest Xylographus Bostrichoides

Zhu F.1 , Li J.S.1 , Xie W.C.1 , Wang C.Y.1 , Lu W.H.2

1Department of Chemical Engineering, Foshan University, Foshan, China; 2School of Life Science and Engineering, Foshan University, Foshan, China

Aims: To investigate the secondary metabolites secreted by the stored products pest Xylographus bostrichoides and to screen for novel insect natural products with antibacterial activity.

Methods: The secretions of X. bostrichoides were collected by feeding them with the medicinal mushroom Ganoderma applanatum (pers.) Pat. The collected secretions were extracted with ethyl acetate, and the crude extract was separated and purified by repeated silica gel column chromatography. The purified compounds were identified by silica gel TLC, m.p., and NMR. The antibacterial activity was tested using a microdilution method.

Results: Two secondary metabolites secreted by X. bostrichoides were isolated and identified as stellasterol (1) and ergosterol peroxide (2) (Fig. 1). The MIC value of compound 1 against S. aureus and E. coli was 2000 μg/mL, and that of compound 2 against S. aureus and E. coli was 1000 μg/mL.

image

Fig. 1 Stellasterol (1) and ergosterol peroxide (2) secreted by X. bostrichoides.

Conclusions: Two steroids were isolated from the secretions of X. bostrichoides, and identified as stellasterol (1) and ergosterol peroxide (2). Both compounds 1 and 2 showed moderate antibacterial activity in vitro against S. aureus and E. coli. These steroids may be insect pheromones or defensins secreted by the fungus beetle, nevertheless, it is also possible that they are indigestible residues derived from the mushroom G. applanatum.

Acknowledgements: This work was supported by a project grant from the Guangdong Provincial Natural Science Foundation of China (Grant No. 2016A030313380).

068

Research on Integrated Interconnection Model of Multi Brains based on Artificial Humoral-Neural Network with Multi Anesthetic/Analeptic Factors

Fan C.

MIT & NJTech Intel, United Lab of “Data Quality and Information Security”, Nanjing Tech University

Aims: Design an interconnection model for multi brains. Based on this, analyze the integrated model for some future or unknown beings and optimize it to apply to other fields.

Methods: We used the artificial self-organizing neural network to simulate the basic functions of the brain. We adopted a number of humoral factors as the intermediaries and the intel-brains feedbacks with multi anesthetic/analeptic factors to interconnect several brains. We adjusted the intel parameters to assure the stability and controllability of the integrated model. We tested the distributed system in different environments and competed with some powerful individual brains based on different centralized strategies. We analyzed the technical feasibility, optimized the model and applied it to some other fields.

Results: The interconnected multi brains can win the competitions and successfully survive in difficult environments.

Conclusions: This integrated interconnection system of multi brains can suit some future or unknown environments and can also be applied to several different kinds of optimization fields.

Acknowledgements: This work was supported by National Natural Science Foundation of China (Grant No. 71671089 and 71171110).

069

Arctigenin Improve Lung Impairment Induced by High Blood Glucose

Xie Z.S.1,2 , Wang H.1,2 , Liu P.1,2 , Wang J.1,2 , Wang J.M.1,2 , Cui Y.1,2

1Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province; 2Pharmacy College, Henan University of Traditional Chinese Medicine

Aim: Long-term chronic hyperglycemia caused the damage of blood vessels or microvascular is the major risk factor for complications of diabetes. In recent years, many studies have shown that the lungs may be one of the target organs of diabetes, and the lung damage of diabetes attracts growing concern. Arctigenin (ARC), a compound isolated from Arctium lappa L., has the effect of anti-inflammatory and anti-diabetic nephropathy. This paper aims to study the role of ARC on alleviate diabetic lung damage.

Methods: After adjusting for 1 week, the rat was given an intraperitoneal inject streptozotocin 65 mg/kg after fasted 4 h. After 2 weeks, fasting blood glucose was measured. Above 16.8 mM was considered diabetic and rats with blood glucose above this level were used for this study. The diabetic animal was randomized during the 16th week and allocated into four groups (n = 10 per group) to receive treatment: 1) streptozocin(STZ) = vehicle, 2) insulin = 1 IU/kg, 3) ARC-H = 50 mg/kg ARC, 4) ARC-L = 12.5 mg/kg, and administration with ARC for 4 weeks. Fasting blood glucose, insulin levels and glycosylated hemoglobin were measured. After the animal sacrificed, the lung tissue was taken for pathological or western-blot analysis.

Results: Insulin or ARC can lower fasting blood glucose, insulin and glycosylated hemoglobin levels in diabetic rats. The pathologic results indicated that thickening of the basal membrane in the endothelial cells and the basement membrane of the alveolar epithelial cells were improved by the ARC in the diabetic rat. Immunohistochemical results suggest that ARC can reduce the expression of TGF-β1 and collagen IV in the basal membrane and alveolar epithelial cells of the pulmonary capillaries of diabetic rat. Western blot indicated that protein expression of TGF-β1, p-Smad3 was decreased after ARC 4-weeks treatment.

Conclusion: The results showed that the ARC was able to reduce lung damage in diabetic rats, possibly through TGF-β1 pathway.

Acknowledgement: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (grant number 2015R1D1A1A09057878).

070

Influence of Polygonum Cuspidatum Anthraquinone and Panax Notoginseng Saponins on RAGE/AGEs Levels in Diabetic Nephropathy RATS Kidney

Wang H.1,2 , Wang J.2 , Liu P.2 , Xie Z.S.2 , Dong B.2 , Liu G.2 , Zhao Z.B.2 , Miao M.S.2

1Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province; 2Pharmacy College, Henan University of Traditional Chinese Medicine

Aim: Based on the study of the intervention effect on the protein expression of receptor for advanced glycation end products (RAGE)/ advanced glycation end products (AGEs) in early diabetic nephropathy model of the compatibility of polygonum cuspidatum anthraquinone and panax notoginseng saponins (PNS) to find the ideal compatibility proportion of Polygonum cuspidatum anthraquinone and PNS, and to explore the mechanism of improving diabetic nephropathy.

Methods: The Sprague-Dawley (SD)rat model of diabetes was induced by intraperitoneal injection of streptozotocin (STZ) (65 mg/kg) and fasting blood glucose level >16.7 mmol/L for successful model, SD model rats were randomly divided into seven groups, normal group, model group, Anthraquinone group,PNS group,Polygonum cuspidatum anthraquinone/ PNS (1:1, 1.5:1, 1:1.5) group. Normal group and model group rats per 100 g weight were administrated with 1 mL normal saline. Polygonum cuspidatum anthraquinone/ PNS(1:1, 1.5:1, 1:1.5) group were given respectively anthraquinone of polygonum cuspidatum anthraquinone (8.75 mg/kg, 13.13 mg/kg, 8.75 mg/kg) and PNS (35 mg/kg, 35 mg/kg, 52.5 mg/kg). Daily stomach irrigation was administered once, medicine for 8 weeks. The expression of RAGE and AGEs in rat kidney was determined by immunohistochemistry, and the morphological changes of the kidney were observed by optical microscope. The strength of the immunohistochemical reaction was shown by Integral Optical Density (IOD).

Results: Compared with model group, the expression of AGEs of the dosage groups were decreased, and 1.5:1 group effect was observed (P < 0.01). The expression of RAGE of dosage groups was decreased, and 1.5:1 group effect was observed (P < 0.05).

Conclusion: Polygonum cuspidatum anthraquinones and PNS can inhibit the expression of RAGE、AGEs by which the function of kidney protection can be acquired and the process of DN can be delay .

Acknowledgements: This work was supported by a project grant from the distinguished talent fund of Henan University of Traditional Chinese Medicine (Grand No.00104087) and the key scientific research project plan of Henan of Institutions of higher learning (Grand No. 17A360017).

071

Characterization and Preparation of the Complex of Hypocrellin B with Modified β-cyclodextrin

Chen R.1 , Yan H.P.1,2,3 , Chen Y.S.1,2,3 , Wang J.1,2 , Yang Y.M.1 , Zhang G.W.1,2 , He Y.1,2,3 , Zhang J.C.1,2,3

1College of Science, Honghe University, Mengzi, China; 2Key Laboratory of Natural Pharmaceutical & Chemical Biology of Yunnan Province, Honghe University, Mengzi, China; 3Local Characteristic Resource Utilization and New Materials Key Laboratory of Universities in Yunnan, Honghe University, Mengzi, China

Aims: Hypocrellin B (HB) is one of the excellent natural photosensitizers, which is isolated from the fungus hypocrella bambusae. To prepare the nanoparticle complex of hypocrellin B (HB) with modified β-cyclodextrin and the photosensitization of the complex also was investigated.

Methods: The β-cyclodextrin was grafted by chitosan under room temperature according to the literature. The 0.5 mg HB was added in 40 mL modified β-cyclodextrin solution (12.5 mg/mL) and mixed in a magnetic stirrer for 7 h at room temperature. Then the complex was dried by a freeze dryer, and the solid inclusion complex was collected.

Results: The β-cyclodextrin grafted product (CTS-β-CD) and the complex of HB with chitosan grafted β-cyclodextrin (HB/CTS-β-CD) were prepared by the lyophilization technique. The FT-IR spectrum indicated that the chitosan was grafted on β-cyclodextrin. The max absorption peak of HB from 468 nm was red shift to 491 nm in the complex.

Conclusions: The inclusion complex of HB with CTS-β-CD was successfully prepared for the first time. The CTS-β-CD can increase the solubility of HB in water. The maximum absorption peak of HB was red shifted to 491 nm, suggesting that the HB is incorporated in the hydrophobic environment of the CTS-β-CD as reported in literatures. The complex of HB with CTS-β-CD has the drug delivery function as reported in literatures, so it may have important applications in photodynamic therapy (PDT).

Acknowledgements: This work was supported by the National Natural Science Foundation of China (Grant No. 21362010) and the Yunnan Natural Science Foundation (2010ZC153).

072

Fully-Automated Lumen Morphology Segmentation and Classification Algorithms for Intravascular Optical Coherence Tomography

Yang S.1 , Han H.J.2 , Lee J.H.1

1Department of Biomedical Engineering, College of Medicine, Kimyung University, South Korea; 2Fashion Design Major, School of Vision, Kimyung University, South Korea

Aims: We propose an algorithm to automatically detect the lumen morphology and to analyze its shape and texture, classifying extracted features as an normal/abnormal lumen using a machine learning technique.

Methods: To detect the lumen morphology, the proposed algorithm transformed the polar into Cartesian coordinates of the IVOCT images. The guide wire shadow region and the stent struts could be detected via pattern analysis and interpolated, and the stent struts shadow region was filled with high pixel values. An active contour model was used to detect irregular lumen morphology.

Results: Abnormal IVOCT images, including an irregular lumen, could be detected and classified in a few seconds, leading to the understanding of the overall vascular status and determination of cardiovascular diagnosis.

Conclusions: The proposed algorithm can help clinicians to better understand the condition of the stented segment. In addition, the protrusion and thrombus can be easily detected.

Acknowledgements: This research was supported by The Keimyung University Research Grant of 2015.

073

Correlation Analysis of the Blood-Lipid Ratio and Homocysteine in Patients with Hypothyroidism Complicated by Coronary Heart Disease

Mu Z.X.1 , Li H.Y.1 , Zhang J.Y.2 , Hou J.Z.3

1Institute of Thyroid Diseases, Cangzhou Medical College, Cangzhou, China; 2Laboratory Diagnostics Division, Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou, China; 3Department of Surgery, Cangzhou People's Hospital, Cangzhou Medical College, Cangzhou, China

Aims: To evaluate the correlation of thyroid hormones, blood-lipid ratio, and homocysteine in patients with hypothyroidism complicated by coronary heart disease.

Methods: Patients were divided into groups based on the presence of hypothyroidism complicated by coronary heart disease (merged group), hypothyroidism, subclinical hypothyroidism, and control individuals, with 50 cases in each group. The levels of serum thyroid hormones, blood lipids, and homocysteine were measured, and the blood-lipid ratio was calculated.

Results: The serum TSH in patients with hypothyroidism and hypothyroidism complicated by coronary heart disease was significantly higher than that of patients with subclinical hypothyroidism or control individuals (< 0.01), however, the levels of FT3, FT4, T3, T4, and HDL-D were decreased (< 0.05). The ratios of TC, TG, LDL-C, TC/HDL-C, TG/HDL-C, LDL-C/HDL-C, and LCI, and the levels of non-HDL-C and HCY were significantly higher in patients with hypothyroidism and hypothyroidism complicated by coronary heart disease than those in patients with subclinical hypothyroidism or control individuals (< 0.05). All parameters were significantly higher in patients with hypothyroidism complicated by coronary heart disease than those in patients with hypothyroidism (< 0.05).

Conclusions: There exist significant abnormalities in lipid metabolism and homocysteine in patients with hypothyroidism complicated by coronary heart disease. Dynamic monitoring of the lipid ratio and cardiac function has important clinical value in patients with subclinical hypothyroidism that has the potential to progress to hypothyroidism, and in predicting hypothyroidism complicated by coronary heart disease.

Keywords: Hypothyroidism complicated by coronary heart disease, Hypothyroidism, Blood-lipid ratio, Homocysteine

Project Funding: The Science and Technology Plan Project in Hebei province (No. 15277796D).

074

Predictive Method of Coronary Heart Disease Gene Based on the Recommendation System

Huang Y.L.1 , Huang Q.2 , Ali S.3 , Liu R.Q.1

1Information center, Beijing University of Chinese Medicine, Beijing, China; 2Respiratory Medicine, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China; 3Department of Computer Science, University of Education, Lahore, Pakistan

Aims: The aim of this study is to predict when genes will cause coronary heart disease (CHD), and provide useful information for early diagnosis and pathogenesis of CHD.

Methods: From the perspective of system biology, the data mining technique and the recommendation system algorithm were used to predict the causal genes of CHD by the degree of functional consistency between disease risk genes and known pathogenic genes.

Results: Twenty-four percent of the target genes in the candidate gene in the top 5%, the target gene in the top 50% candidate genes. The method can effectively identify the pathogenesis of CHD genes.

Conclusions: This study summed up the research and application of the recommended system in the literature of CHD, and proposed the research idea in the prediction of disease genes.

Keywords: recommendation system, prediction of disease genes, coronary heart disease, knowledge discover

Acknowledgements: This work was supported by a project grant from Beijing University of Chinese Medicine (Grand No. 2016-JYB-QNJSZX0005).

075

Training Methods and Structural Design of an Intelligent Knee Prosthesis Based on Physiological Gait

Cao W.J.1,2 , Yu H.L.1,2 , Zhao W.L.1 , Wei X.D.1

1University of Shanghai for Science and Technology; 2Shanghai Engineering Research Center of Assistive Devices

Aims: To investigate the damping adjustment laws of an intelligent knee prosthesis in order to achieve physiological gait.

Methods: Physiological gait was evaluated by measuring the knee joint angle curve of healthy individuals at low (0.8 m/s), medium (1.2 m/s), and high (1.8 m/s) speeds. A novel type of single-axis hydraulic damping knee prosthesis was designed, and training methods were proposed related to the stance and swing phase. A knee prosthesis test was performed using a simulation and measurement device.

Results: The maximum swing-phase knee-flexion angle in healthy individuals was 60° to 70° depending on the speed. When the damper valve closure was 0%, the maximum swing-phase knee-flexion angles of the knee prosthesis were 86°, 91°, and 91° at 0.8 m/s, 1.2 m/s, and 1.8 m/s, respectively. Once the valve closure percentage was changed, the maximum swing-phase knee-flexion angle could be adjusted to between 60° and 70° at different speeds, and the required valve closure percentages were 25%, 40%, and 70% at 0.8 m/s, 1.2 m/s, and 1.8 m/s, respectively.

Conclusions: When the flexion damping valve was fully open, the maximum swing-phase knee-flexion angle increased with the speed augmentation. The required valve closure percentages were different at the various speeds. The higher the speed, the larger the valve closure percentage, and the smaller the valve opening.

Acknowledgements: This work was supported by a project grant from The National Natural Science Foundation of China (Grant No. 61473193).

076

Study on the Effect of Silicate Bacterial Metabolites on Experimental Asbestosis

Zhang J.1 , Cheng W.B.1 , Liu Y.J.1 , Wu S.Z.2 , Pu D.3 , Liu H.1 , Zhao Y.J.1 , Liu H.R.1 , Wang Y.1

1West China School of Public Health, Sichuan University, Chengdu, China; 2West China School of Clinical Medical, Sichuan University, Chengdu, China; 3West China Hospital of Sichuan University, Chengdu, China

Aims: To evaluate the protective effect of silicate bacterial metabolites on rats that have inhaled asbestos.

Methods: Eighty healthy male rats were divided into groups; normal control (n = 5), treatment control (n = 5), asbestos-inhaled (n = 35), and asbestos-inhaled treated (n = 35). For the asbestos-inhaled group, the asbestos lung model was established using ultrasonic atomization of asbestos dust, and the rats were subsequently treated with ultrasonic atomization of metabolites from silicate bacteria. For the normal group, the rats inhaled nebulized distilled water. For the treatment and control groups, the rats were treated with ultrasonic atomization of silicate bacterial metabolites. Rats were sacrificed on the corresponding day. Lung and blood samples were extracted for pathological examination. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), and hydroxyproline (HYP) were evaluated. The results were analyzed using the statistical software PM3.0.

Results: There were no abnormalities in the control group, and the treatment control group was similar, with the exception of a slight occasional inflammatory reaction. Mild acute inflammation could be seen in the asbestos-inhaled group at a early stage, which became chronic inflammation with fibrous tissue hyperplasia, and over time the fibrosis became more serious. In the asbestos-inhaled treated group, the inflammatory reaction was very minor and only slight fibrous tissue hyperplasia could be seen. There were no significant differences in the levels of MDA, SOD, or HYP between the normal control group and treatment control group, as well as the lung index. However, the SOD, MDA, HYP of asbestos-inhaled group and asbestos-inhaled treated group were higher than those of the normal control group. While the MDA and HYP in the asbestos-inhaled treated group were lower than those in the asbestos-inhaled group, however, the levels of SOD were higher in the former.

Conclusions: The silicate bacterial metabolites slowed down the development of asbestosis.

Acknowledgements: This work was supported by a project grant from The Sichuan Science and Technology Support Plan (Grant No. 2011SZ0110).

077

The Antagonism of Agmatine on Inflammatory Lesions and Sepsis

Ma X.Y. , Liang H.P.

State Key Laboratory of Trauma, Burns, and Combined Injury, Research Institute of Surgery, The Third Military Medical University, Chongqing, People's Republic of China

Aims: Agmatine (AGM) has extensive cytoprotective properties including neuroprotection, cardiac protection, and renal protection, which have been fully established by several research studies. Considering that studies on AGM are limited with respect to sepsis and inflammatory lesions, we aimed to evaluate the protective effect of AGM on acute lung injury, acute peritoneal inflammatory injury, and sepsis in C57BL/6 mice.

Methods: The models of acute lung injury and acute peritoneal inflammatory injury were reproduced in C57BL/6 mice by zymosan (ZYM, 500 mg/kg). Cecal ligation and puncture (CLP) was conducted to establish a sepsis model in C57BL/6 mice. Histological examination of the lung was carried out and evaluated on a scale from 0 to 3. The magnitude of pulmonary edema was evaluated by the wet/dry weight ratio. Myeloperoxidase (MPO) activity in the lung tissue was detected using a commercial kit. Cytokine production in the three above-mentioned animal models was measured by ELISA. A TransAM NF-κB p65 transcription factor assay kit was used to quantify the DNA binding activity of NF-κB in lung tissue.

Results: AGM (200 mg/kg) relieved acute lung injury induced by ZYM, resulting in a lower W/D weight ratio, lower histological scores, and decreased MPO activity in the lung tissue. The levels of TNF-α, IL-1β, and IL-6 in the lung and BLF were also decreased. Furthermore, AGM dramatically reduced the levels of KC, MIP-2, TNF-α, and IL-6 in the serum and peritoneal lavage fluid following ZYM-induced acute peritoneal inflammatory injury. In the CLP model, AGM dramatically decreased the serum levels of vascular endothelial biomarkers (ICAM-1, VCAM-1, VEGF, vWF, Ang-2, and MCP-1) and inflammatory cytokines (IL-6, TNF-α, and IL-10). The potential mechanism of AGM in ZYM-induced inflammatory injury was confirmed to be blockade of the activation of NF-κB by inhibition of the phosphorylation and degradation of IκB.

Conclusions: AGM inhibited the activation of the NF-κB signaling pathway, in turn protecting mice against acute lung injury, acute peritoneal inflammatory injury, and sepsis. This suggests that AGM may be a potential agent for the treatment of inflammatory disorders.

Acknowledgements: This study was supported by grants from The National Basic Research Program of China (973 Program) (No. 2012CB518102), The Key Project of the “Twelfth Five-Year” Plan for Medical Science and Technology of PLA (No. BWS11J038), and The Special Fund from PLA (2014ZX09J14107-08C).

Keywords: Agmatine; acute lung injury; acute peritoneal inflammatory injury; sepsis.

078

Aberrant Glycosylation in a Metastatic Cell Model of Salivary Adenoid Cystic Carcinoma

Lv L.Y. , Chen W.R. , Cheng A.Y. , Yu Y.N. , Wen C. , Zhang L.

Institute of Biomedical and Pharmaceutical Sciences, Hubei Key Laboratory of Industrial Microbiology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan, China

Aims: To identify metastasis-associated glycosylated proteins in salivary adenoid cystic carcinoma (SACC), and to provide potential clinical signatures and therapeutic targets for SACC.

Methods: A smart-polymer-based material was used to pull down glycosylated proteins in low or high metastatic SACC cell lines. Enriched proteins were separated and profiled using two-dimensional electrophoresis (2-DE). Proteins with a differential glycosylation status between the two cell types were identified using liquid chromatography-mass spectrometry (LC-MS). Selected glycosylated proteins were further subjected to functional analysis to assess SACC metastasis using typical molecular biological and cell biological techniques.

Results: Hundred and twenty-nine glycosylated proteins were shown to be predominantly expressed in low metastatic SACC cells, and 130 in high metastatic cells. LC-MS analysis of selected electrophoretic dots revealed that the urokinase receptor for plasminogen activator (PLAUR) was highly glycosylated in high metastatic SACC cells, which was also confirmed in clinical SACC specimens. A mutagenesis assay revealed that a specific glycosylation pattern of PLAUR was associated with SACC metastasis.

Conclusion: Aberrant glycosylation was associated with SACC metastasis and provided a novel strategy for glycosylation analysis in metastasis,

Acknowledgements: This work was supported by grants from The Natural Science Foundation of China (31501150) and The Wuhan Youth Science and Technology Plan (2016070204010140).

079

Observation of the Clinical Effect of the Fuzhengpaidu Tablet in HIV-Infected Individuals

Li Q.Y.1 , Wang X.Y.1 , Xu Q.L.2 , Li Q.2 , Jin Y.T.2 , Guo H.J.2

1Henan University of Chinese Medicine, Zhengzhou, China; 2The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, China

Aims: To observe the clinical effect of the Fuzhengpaidu tablet on HIV-infected patients.

Methods: A double-blind, randomized, placebo-controlled clinical trial was designed for 96 patients who had had a HIV infection for 6 months. The patients were randomly divided into a control group (48 cases) and a treatment group (48 cases), using the Fuzhengpaidu tablet or placebo, respectively, for 6 months. CD4+ and CD8+ cell numbers, in addition to symptoms, signs, and integral improvement rates, were observed to evaluate the clinical effect of the Fuzhengpaidu tablet.

Results: The Fuzhengpaidu tablet significantly improved the self-conscious symptoms of patients, increased their CD4+ and CD8+ T cell count, and improved patient's immunity. The Fuzhengpaidu tablet was safe under existing experimental conditions. During the clinical trial we did not find any side-effects or adverse drug reactions.

Conclusions: The Fuzhengpaidu tablet significantly improved the self-conscious symptoms and immunity of HIV patients.

Acknowledgements: This work was supported by grants from The International S&T Cooperation Program of China (Grant No. 2007DFB31610), The National Natural Science Foundation of China (Grant No. 81603607), The Henan Province Natural Science Foundation (Grant No. 162300410189), The Science and Technology Project of Henan Province (Grant No. 142300413215), and The Science and Technology Project of Henan Province (Grant No. 102300413212).

080

Design of Artificial Pancreas with Self-Regulated and Prolonged Insulin Release Behavior

He J.1 , Bai M.R.2 , Huang C.1 , Yin R.X.1,3 , Zhang W.J.1,3

1Complex and Intelligent Systems Research Center, East China University of Science and Technology, Shanghai, China; 2State Key Laboratory of Chemical Resource Engineering & Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, China; 3Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Canada

Aims: The self-regulated delivery of insulin according to changes in blood glucose levels and the prolonged supply of insulin are important for diabetic patients; however, these goals have not yet been combined in a small implantable chemically-controlled system [1-2]. The purpose of the present research was to design and engineer synthetic pancreas capable of releasing insulin in a glucose-responsive and continuous manner for diabetes care.

Methods: The developed artificial pancreas was composed of linear degradable chitosan hydrogels integrated with glucose-responsive microspheres. We used the specific affinity of concanavalin (ConA) for sugar ligands to develop glucose-responsive microspheres for self-regulated insulin delivery, and, embedded them in chitosan scaffolds with a specifically-designed structure. To test in vitroinsulin release, we applied a glucose stimulus cycle to simulate the physiological bolus and basal insulin release, in addition to prolonged release over more than 10 days. The bioactivity of the released insulin and cell cytotoxicity were also investigated.

Results: In vitro release experiments indicated that the microspheres-loaded scaffolds can not only release insulin based on the fast-responding microspheres, but also prolong the self-regulated release behavior. With respect to the degradation of the scaffolds, the insulin-loaded microspheres can be gradually leaked into the environment to serve a glucose-responsive function over a long period of time. The released insulin was proven to remain active, and the material showed no cytotoxicity towards the growth of human dermal fibroblasts (HDF) cells, indicating good in vitro biocompatibility.

Conclusions: The designed artificial pancreas was capable of releasing insulin in a glucose-responsive and continuous manner.

Acknowledgements: This work was supported by a project grant from The National Natural Science Foundation of China (21404038) and the Fundamental Research Funds for the Central Universities (22A201514029).

References:

[1] Hui Yu Huang, Simon Chiang, Geoffrey A. Ozin, Adria Giacca, and Xiao Yu Wu. Nanotechnology-Enabled Closed Loop Insulin Delivery Device: In Vitro and In Vivo Evaluation of Glucose-Regulated Insulin Release for Diabetes Control. Advanced Functional Materials, 2011, 21, 73–82.

[2] JIN DI, JICHENG YU, YANQI YE, DAVIS RANSON, ABHILASHA JINDAL, and ZHEN GU. Engineering Synthetic Insulin-Secreting Cells Using Hyaluronic Acid Microgels Integrated with Glucose-Responsive Nanoparticles. Cellular and Molecular Bioengineering, 2015, 8, 445-454.

081

Bioinformatics Network Analysis to Elucidate Key Pharmacogenetic Genes Involved in Type 2 Diabetes in the Northern Chinese Han Population

Pei Z.Y.1,2 , Liu M.J.1,2 , Chen Y.B.1,2

1Beijing Computing Center, Beijing Academy of Science and Technology; 2The Key Laboratory of Beijing Cloud Computing Technology and Application

Aims: Type 2 diabetes mellitus (T2DM) is a complex disease resulting from a combination of factors. Many pharmacogenetic and susceptibility genes have been reported, however, it has proven challenging to elucidate key genes in the relevant biological pathways and networks. Here we employ a bioinformatics network analysis approach to elucidate these key genes involved in T2DM in the Northern Chinese Han population.

Methods: We used bioinformatics tools to evaluate the key pharmacogenetic genes, and validated those with genotyping in the Northern Chinese Han population, containing over 1000 individuals, using target DNA sequencing. The list of pharmacogenetic genes was obtained from HGMD (The PharmacoGenomic Mutation Database) and Pharmgkb (The Pharmacogenomics Knowledge Base) by a Perl script that we coded. The susceptibility genes for T2DM were obtained from HGMD (The Human Gene Mutation Database, Profession). We used bioinformatics methods and tools, such as IPA, to build a pharmacogenetic gene network. Subsequently, by overlapping the susceptibility gene networks, we were able to define the hub nodes in the network, and to highlight the key genes with the most degrees.

Results: The pharmacogenetic genes were highlighted in the network that we built, such as PPARG, which has a high degree, and thus, was considered not only strongly related to T2DM, but more importantly, it has been reported to be the target of the Pioglitazone test in Asian people with a particular SNP. To validate this in the Northern Chinese Han population, we carried out an experiment using target DNA sequencing with 1062 T2DM patients. The results were statistically significant (< 10-8).

image

Figure 1. The highlighted key pharmacogenetic genes are shown in the network overlapping with T2DM susceptibility genes.

Conclusions: It is useful and feasible to evaluate the key genes involved with T2DM by network analysis using bioinformatics methods and tools. Certain key pharmacogenetic genes, such as PPARG, may play important roles in T2DM genetics in the Northern Chinese Han population.

Acknowledgements: This work was supported by grants from The Key Project of Beijing Talents and Youth (Grant No. 2015400685627G238), The BJAST Young Backbone Project (Grant No. BJASTYT201620), and the BJAST Innovation Team Project (Grant No. IG201406C2).

082

The Protective Effects of Probiotic-Fermented Ginseng on High Fat Diet-Induced Hypercholesterolemia

Nan B. , Wang X.Z. , Li W.C. , You Y. , Fan J.J. , Liu M.Y. , Wang Y.S.H. , Wang Y.H.

College of Food Science and Engineering, Jilin Agricultural University, Changchun, China

Aims: Ginseng is a traditional herbal medicine with many pharmacological actions such as anti-inflammatory, anti-tumor, and hypolipidemic activities. We have previously proven that probiotic-fermented ginseng can reduce high cholesterol levels induced by a high fat diet. Moreover, the flavor of probiotic-fermented ginseng was improved. However, the underlying mechanisms of probiotic-fermented ginseng in the prevention of high cholesterol levels are not yet fully understood. The aim of this study was to elucidate the pathway by which probiotic-fermented ginseng prevented hypercholesterolemia in mice fed a high fat diet.

Methods: Lactobacillus fermentum was screened for the fermentation of ginseng. C57BL/6 mice were randomly divided into three groups and fed different diets for 15 weeks. A) Control group (Control): normal chow; B) High fat diet group (HFD): high fat diet; and C) Probiotic-fermented ginseng group (HFD+FG): high fat diet supplemented with probiotic-fermented ginseng (450 mg/kg/day of FG).

Results: Probiotic-fermented ginseng dramatically reduced the increase in serum TC and LDL levels induced by a high fat diet. Further, probiotic-fermented ginseng decreased the abdominal fat index of high fat diet-fed mice. It is generally known that cholesterol is involved in lipogenesis. The increase in serum TC levels was related to abdominal fat accumulation in high fat diet-fed mice. In addition, the increase in TC and LDL levels directly showed hypercholesterolemia. Moreover, probiotic-fermented ginseng remarkably increased serum leptin levels. The levels of leptin significantly affected cholesterol metabolism, and probiotic-fermented ginseng promoted leptin secretion in high fat diet-fed mice. These results indicated that probiotic-fermented ginseng improved high fat diet-induced hypercholesterolemia through adjustments to the leptin pathway.

Conclusions: Probiotic-fermented ginseng was effective in the prevention of high fat diet-induced hypercholesterolemia.

Acknowledgements: We are grateful to The Jilin Province Sci-Tech Department, P.R. China for providing financial support (Grant No. 20140519011JH).

083

Research on a Health Monitoring System Based on a Wireless Network

Dou R.L.1 , Su Z.2 , Shi W.J.3

1School of Software Engineering, Jinling Institute of Technology, Nanjing, China; 2Center of Information Construction and Management, Jinling Institute of Technology, Nanjing, China; 3Department of Equipment Management, Jinling Institute of Technology, Nanjing, China

Aims: Health monitoring is conducive to disease prevention and early treatment, reducing medical costs. We applied wireless network technology and an intelligent algorithm to personal health monitoring and management.

Methods: We built a smart mobile health monitoring system based on wireless networks. The system uses personal wearable devices to obtain the main physiological parameters of the human body, and the measured data is sent to the server through the wireless network. An intelligent analysis server then sends the results back to the monitoring personnel.

Results: The system can help people to understand their health status over time and provide reliable data for disease diagnosis.

Conclusions: Through the application of wireless network technology, a wearable device, and an intelligent analysis method, the working efficiency of medical staff can be improved along with health. Primary occurrence and reoccurrence of common and chronic diseases can be prevented, as well as an improvement in the quality of life, a reduction in medical costs, and achievement of a full range of health management.

Acknowledgements: This work was sponsored by The Project of Modern Educational Technology of Jiangsu Province (2017-R-54608, 2017-R-54927).

084

A Comprehensive Level Dependable Adaptive Wavelet Thresholding Algorithm for ECG Denoising

Rahman M.S. , Choi C. , Kim Y. , Kim S.

Kongju National University

Aims: Portable electrocardiogram (ECG) devices are very popular for measuring heart activity. However, ECG signals are corrupted by several artifacts such as power lines, baseline drift, and motion artifacts. These artifacts hamper the real diagnosis process for heart diseases. In this paper, we proposed a wavelet transformation method for subtracting noise from ECG signals.

Methods: Wavelet transformation is one of the popular techniques for ECG denoising. Choosing an appropriate threshold for noise subtraction is a difficult task. The proposed algorithm was based on Daubechies 4 discrete wavelet functions, which adapts the threshold value at each level to subtract noise from ECG signals. Each wavelet decomposition level has two coefficients; details and approximation. The maximum and the minimum values for the approximation coefficient (Ac) were calculated. The threshold value is defined as Tnext = Tprevious + max(Ac)\min(Ac). When the approximation coefficients (Ac) is greater than the threshold value, the threshold value will be subtracted from the approximation coefficients .But on the other hand, if the approximation coefficient (Ac) is less than the threshold value, the approximation coefficients (Ac) will be zero .

Results: To evaluate our algorithm we added non-leaner and leaner noise with 20 MIT/BIH ECG data. We used the Matlab wavelet toolbox for comparison of our method using different thresholding methods. The average SNRs (signal to noise ratios) of the wavelet fixed, heuristic, and minimax rigorous thresholding methods were -14.4267 db, -12.2267 db, -14.467 db, and -13.4267 db, respectively. The average SNR of the proposed algorithm was -4.8801 db. These results indicate that our method is better than other thresholding methods.

Conclusions: In ECG signal analysis, the signal character is essential. In this study, we tried to minimize the ECG denoising error based on the Daubechies 4 wavelet method using adaptive thresholding. The experimental results show that the proposed algorithm was more effective than the fixed, heuristic, or minimax rigorous thresholding methods and helping to detect heart diseases more effectively.

085

A First Order Derivative UV Spectrophotometric Method to Measure the Deacetylation Degree of Chitooligosaccharides

Jiang Y. , Guo J. , Su Z.Q.

Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, China

Aims: To evaluate a method for the assessment of the deacetylation degree (DD) of chitooligosaccharides (COS) with relatively high and low molecular weights, since the methods for the determination of chitosan content recommended by the Chinese Pharmacopoeia are not applicable.

Methods: A first order derivative UV spectrophotometric method for the assessment of the DD of COS was established, with the best-suited solvent being 0.3 M hydrochloric acid. The accuracy of this method as a function of molecular weight was also investigated and compared with results obtained using 1H NMR spectroscopy.

Results: The COS samples were divided into low and high molecular mass groups, those with average molecular weights of ≤1000 Da (COSA) and ≤3000 Da (COSB). The DD of both groups were evaluated. The two samples were subjected to first order derivative UV spectrophotometry, yielding DD values of 93.44 ± 0.54% and 92.88 ± 0.43% (n = 6), respectively. These values were consistent with those determined by 1H NMR spectroscopy, which were 93.54 ± 0.16% and 92.85 ± 0.13% (n = 6).

Conclusions: This study demonstrated a widely adaptable, highly precise, accurate, and inexpensive method for the determination of the DD of COS, which have the potential for widespread commercial application.

Acknowledgements: This project was supported by The Science and Technology Planning Project of Guangdong, China (No. 2013B021100018), The Industry-University-Research Collaborative Innovation Major Projects of Guangzhou The Science Technology Innovation Commission, China (No. 201604020164), and The National Science Foundation of China (No. 81173107).

086

Brain Tumor Image Segmentation Based on Morphology

Gong T. , Wu J.

College of Information Science and Technology, Engr. Research Center of Digitized Textile & Fashion Tech. for Ministry of Education, Donghua University, Shanghai, China

Aims: To investigate the accurate segmentation of brain tumor images and to decrease the possibility of over-segmentation, an improved watershed algorithm was proposed with morphological gradient images and simulated with Matlab.

Methods: The improved watershed algorithm was built with the opening and closing operators of the morphological image-filtering. With the calculation of structural elements, the morphological gradient-related structural elements determined the radius, which could be modified according to the multi-scale morphology. The tumor target region of the brain image could be marked correctly with the proposed improved watershed segmentation method, as shown in Fig. 1.

Results: The direct watershed segmentation resulted in a serious over-segmentation phenomenon, thus, the brain segmentation image was useless. This over-segmentation of the brain tumor image was decreased by the improved watershed algorithm, as shown in Fig. 2. This brain segmentation image was marked around the tumor by modifying the gradient map image with the mandatory minimum value and using the distance transformation image according to the Euclidean distance transformation.

Conclusions: The improved morphology-based watershed algorithm was useful in overcoming the over-segmentation phenomenon of the brain image in order to detect the tumor in the brain accurately and quickly.

Acknowledgements: This work was supported by project grants from The National Natural Science Foundation of China (Grant No. 61673007, 61271114, and 61203325) and Innovation Program of Shanghai Municipal Education Commission (No. 14ZZ068).

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Fig. 1. Marked segmentation

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Fig. 2. Comparison of image segmentation

087

Design, Synthesis and Activity of Novel Quinazolines Derivatives as Dual EGFR/C-Met Inhibitors

Wang C.L. , Zhang B.L. , Han J.Q. , Xiong Y.J. , Guo Y.P. , Xu S.

Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science & Technology Normal University, Nanchang, P.R. China

Aims: Epidermal Growth Factor Receptor (EGFR) have become an important therapeutic option for treating several forms of cancer. However, a new challenge, drug-resistance, is coming to us along with the pharmacotherapy of some commercialized anti-cancer drug targeting EGFR (e.g., Gefitinib, Tarceva). Now, several EGFR/c-Met inhibitors of overcoming this resistance have been reported. In this research, we designed and synthesized a series of novel compounds as EGFR/c-Met inhibitors.

Methods: In order to explore the effect of conjugation on the anti-tumor activity,hydrazine was used to extend the Michael addition receptors. Based on the results of our previous research, a series of quinazolines derivatives had been designed and synthesized as novel EGFR/c-Met inhibitors to efficiently overcome the EGFRT790M resistance, and the compounds were evaluated for the IC50 against four cancer cell lines (HepG2, A549, MCF-7 and PC-3) and EGFRT790M. The structures of these target compounds were confirmed by MS and 1H NMR.

Results: In summary, we have designed and synthesized a series of quinazoline derivatives which were tested for their anticancer activities against HepG2, A549, MCF-7 and PC-3 and EGFRT790M inhibitory activities. Ten outstanding target compounds exhibited the anticancer activity toward EGFR/c-Met at 10 nM level.

Conclusion: A collection of the extended Michael addition receptors of Afatinib derivatives were obtained. The anticancer activity of the novel Afatinib derivatives is almost equal compared with the lead compound Afatinib. It is vital for us to design and synthesize the novel EGFR/c-Met inhibitors.

Acknowledgements: This work was supported by The National Natural Science Funds of China (No. 21662014), Outstanding Youth Foundation of Jiangxi, Natural Science Foundation of Jiangxi, China (20171BCB23078), Natural Science Foundation of Jiangxi, China (20171ACB21052 & 20171BAB215073),Science and Technology Project Founded by the Education Department of Jiangxi Province (GJJ160787),College Students’ Science and Technology Innovation Project of Jiangxi Science & Technology Normal University (20150904040).

088

Study on the Chemical Composition of Hippeastrum Vittatum

Ji Y.B.1,2

1Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin, China; 2Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin, China

Aims: Hippeastrum vittatum, a perennial evergreen herb, belongs to the Hippeastrum. Hippeastrum vittatum goes into liver, spleen and lung channels with the characteristic of sweet, acrid, warm, as well as small toxicity. Hippeastrum contains a variety of chemical constituents, including polysaccharides, flavonoids and their glycosides, alkaloids and glycosides, fatty acids, terpenoids, steroids, amino acids and other trace elements. Clinical studies also show that hippeastrum has the effect of invigorating the circulation of detoxification and blood stasis detumescence. It has been reported that the bulbs of amaryllis contain a large number of biologically active ingredients. But the active ingredients in its leaves have not been reported until now. This paper, aiming to provide a theoretical basis for the further development and use of hippeastrum, is intended to study the chemical composition of hippeastrum dry leaves and their pharmacological activity.

Method sand Results: We used heat reflux extraction to obtain chemical compositions in hippeastrum. Solvent extract followed by petroleum ether, methylene chloride, ethyl acetate and n-butanol. We used an 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to detect the four extracted parts on HepG-2 cells in order to choose a better inhibitive part. We separated and purified the compounds in dichloromethane section by multiplex chromatography and then mixed the liquids with the same Rf value as the same sample fractions. We analyzed the purity of the components by HPLC gradient elution with C18 column and methanol - water system as the mobile phase at the flow speed of 0.8 mL/min. We recorded the ratio of the mobile phase when the compound peak separated at the baseline. In this chromatographic condition, preparative HPLC can be used to obtain monomer compounds at the flow rate of 2.0 mL/min. Finally, NMR and MS are used to identify the compound's structure and the inhibitive proliferative effect of the compounds is detected on HepG-2 cell line by MTT assay.

Conclusions: The MTT assay results show that the four parts extraction of amaryllis leaves significantly and dose-dependently inhibit HepG2 cellular proliferation. After silica gel column chromatography, Spell out at first mention (ODS) column chromatography, HPLC analysis and preparation, three compounds were acquired. According to the date of 1H-NMR, 13C-NMR, MS and other spectroscopic data along with the physical and chemical properties, two compound structure are identified, namely 7,3′-dihydroxy-4′-methoxy-flavan and 7,4′-dihydroxy-3′-methoxy-flavan. These two compounds were isolated from Hippeastrum. While one unidentified compound may be a flavonoids probably a new one, further research through two-dimensional spectrum is needed.

Acknowledgements: This work was supported by Harbin city technology bureau project (2016RQQXJ124); Harbin city technology bureau project (2016RAXXJ067); Heilongjiang university innovation talent project (UNPYSCT-201681).

089

Progress in the Identification of Tumor Suppressor Genes in the Diagnosis and Prognosis of EOC

Feng J.1 , Hou J.Z.2 , Yu J.1

1Gynaecology Department 2 of Cangzhou Central Hospital, Cangzhou; 2Department of Surgery Cangzhou People's Hospital, Cangzhou Medical College, Cangzhou

Objective: To summarize the progress in the identification of tumor suppressor genes in the diagnosis and prognosis of EOC.

Method: A literature review was carried out to collect and analyze papers describing EOC-related tumor suppressor genes.

Results: A decrease in the expression rate of the PTEN protein in EOC tissues was negatively correlated with Topo IIa expression, the expression of the P16 protein was negatively correlated with the degree of tumor differentiation, and the negative expression of p27 was significantly correlated with a relative lower survival rate. Mutations in the BRCA1 and p53 genes commonly occurred in late stage EOC, the more differentiated the cells, the later the stage, and the higher the rate of mutant P53. The expression of the protein nm23 was an early event in EOC, and overexpression of C-erbB2 indicated a poor prognosis. The expression of the c-myc protein in EOC was significantly higher than that in benign tumors, and the expression was increased gradually with an increase in EOC stage and degree of tumor differentiation. The majority of the overexpressed proteins in EOC were related to survival, which was correlated with histological type and grade, rather than tumor stage, survival rate, or expression of Bcl-w, Bcl-x, or Bax.

Conclusion: ROC curves were used to optimize 3–4 ideal genetic markers based on the comprehensive detection of EOC-related genes in the diagnosis and prognosis of EOC.

090

Research Status of Breast Cancer Animal Model

Miao M.S. , Tian S. , Bai M. , Xing L.L. , Lou X. , Kang L.

Department of Pharmacology, Henan University of Chinese Medicine, Zhengzhou, China

Aims: We summarized and analyzed the existing breast cancer animal models, and summarized the commonly used experimental animal and animal models of breast cancer, to provide the reference for the research of breast cancer.

Methods: By comparing the literature of the past 10 years, we used the method of classification induction and horizontal comparison, and the experimental animal and animal model of breast cancer animal model were counted. To better understand the current research status of the breast cancer animal model, it would provide an ideal animal model for the study of breast cancer.

Results: The study has found that the experimental animals of the breast cancer animal models commonly used had the BALB/c nude mice without thymus, BALB/c mice, TA2, SD rats, Wistar rats, C3H, Jinbai white spontaneous breast cancer in mice, C57 mice. However, due to large individual differences among the rats, and the morphological characteristics of tumor cells had the large differences, so the rats less used in screening of the anti-tumor drug activity. The mice had the smaller individual differences, the mice is more suitable for the screening of the anti-tumor drug activity. The current breast cancer animal models can be divided into the following categories: spontaneous, induced, transplanted, transplanted by genetic engineering, and the distant metastasis of breast cancer animal model. At present, the chemically induced and the transplanted animal model of breast cancer are used commonly, and the dimethyl phenyl anthracene or methyl nitrosourea was the main chemical carcinogen in the chemical induction method. The animal or human breast cancer tissue and the cell lines were commonly used in the transplanted animal model of breast cancer. This animal model is transplanted in animals and the formation of breast cancer model.

Conclusions: The existing breast cancer animal model reflected the clinical standard or performance in a certain degree, but most of them could not fully reflect the clinical features, the model should be based on clinical standards to improve the corresponding animal model, and established more appropriated animal model for the clinically effective treatment provides new treatments.

Acknowledgements: This work was supported by a project grant from Henan Science and Technology Innovation Team (Grand No. 2012IRTSTHN011) and Science and technology innovation team of Zhengzhou city (Grand No. 131PCXTD612).

091

Enhanced Specific Immune Responses of HPV16-E7 by Combined Intranasal Immunization with Lymphotoxin

Chen H.L.1 , Liu Y.H.1 , Xu B.Q.1 , Ma Z.N.2 , Wei K.2 , Ying X.Y.1

1Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China; 2The Second Clinical Medical College, Nanjing Medical University, Nanjing, China

Aims: Cervical cancer is the leading cause of cancer-related deaths among women worldwide. Human papillomavirus (HPV) is a common infection among women and a cause of cervical cancer. In this study, we evaluated whether lymphotoxin (LT) could enhance specific immune responses of HPV16-E7 in mice.

Methods: Mice were randomly divided into four groups: Control, LT, HPV-E7 and HPV-E7 + LT. Serum and vaginal washes were collected following intranasal immunization with HPV-E7 and/or LT to examine the adjuvant properties of LT and cytotoxic T-lymphocyte (CTL)-mediated cytotoxicities in HPV-E7-specific immune response. In addition, immunohistochemistry was used to assess the capacity of protective T-cell subpopulations [homing] to genital mucosa and associated lymphoid tissues in response to HPV-E7 and LT immunization.

Results: HPV-E7-specific serum IgG and vaginal IgA titers were significantly increased following intranasal immunization with HPV-E7 and LT compared with HPV-E7 immunization alone. The combination of HPV-E7 peptide and LT-induced strong E7-specific CTL responses resulted in more lymphocyte homing factors.

Conclusions: Lymphotoxin may be an effective adjuvant for intranasal immunized HPV-E7 to elicit extremely high E7-specific immune responses to HPV infection. This strategy provides a platform for the development of a new approach to eradicate chronic HPV infection and is capable of inducing an effective immune response to anti-infection.

092

Detection of the Morphology of the Coronary Artery using Real-Time Photoacoustic Tomography In-Vivo

Lee M.Y. , Palaniappan P. , Go S.K. , Park S.H. , Song C.G.

Advanced Biomedical Imaging Center, Chonbuk National University, Jeonju-si, Jeonbuk, South Korea

Aims: To detect the morphology of the coronary artery in a healthy female swine using in-vivo photoacoustic tomography (PAT), which can detect the morphology of vasculature using the optical coefficient of components since the target absorbs the pulsed laser and generates an ultrasound signal.

Methods: A 950 nm pulsed laser wavelength, which is the peak absorption coefficient of HbO2, was employed. The anesthetized swine was placed on a surgical bed and incised over the left side of the respiratory tract in order to expose the coronary artery. To avoid infection, the ultrasound transducer and fiber optic light guide were sterilized. The laser power was limited to 9 mJ/cm2.

Results: Photoacoustic signals were acquired through an ultrasound transducer and reconstructed using a back-projection algorithm. The ultrasound (US) device located the position of the artery and detected its structure, and overlaid the images with the PAT images. PAT detected the structure and the intravascular properties of the artery, which were the arterial wall and the blood, respectively. Finally, following combination of the US and PAT images, we showed both the structure and intravascular properties of the coronary artery.

Conclusions: The morphology of the coronary artery could be detected using PAT in-vivo. PAT will be able to diagnose intravascular disease such as plaques and thrombi using the appropriate wavelength.

Acknowledgements: This work was supported by The Technology Innovation Program (No. 10052749, the development of an ultrafast cardiovascular diagnostic system based on multifunctional 3D ultrasound imaging) funded by the Ministry of Trade, Industry, and Energy (MOTIE) of Korea, and by The Brain Research Program through the NRF (No. 2017M3C7A1044819; 2017R1A4A1015681) funded by the Ministry of Science, ICT, Future Planning, and the Korean government (MSIP).

093

Immunity-Inspired Medical Assistant Robots

Gong T.1 , Long Y.L.1 , Li L.2

1College of Information Science and Technology, Engr. Research Center of Digitized Textile & Fashion Tech. for Ministry of Education, Donghua University, Shanghai, China; 2Department of Gastroenterology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

Aims: To aid doctors and nurses in medical treatments and the healthcare of patients, novel medical assistant robots (MARs) were proposed to be capable of path planning, obstacle avoidance, and immune control.

Methods: MARs guided the patients’ recovery in the hospital by monitoring their health statuses and providing advice in the medical expert sub-systems. MARs also supervised the hospital and kept tight security in the detection of intruders. An immunity-based colony optimization algorithm was used for global path planning to find the relatively shortest non-collision paths for the MARs. The objective function for the path planning was math formula where f(x) was the objective function and gi(x) was nonlinear constrains. Ultrasonic and infrared sensors were used to measure the distances between the MARs and obstacles. The MARs avoided obstacles according to the workflow shown in Fig. 1. A double closed loop immune controller was designed to control the behavior of the MARs, as shown in Fig. 2.

Results: The MARs accomplished path planning by avoiding obstacles, and aided the doctors, nurses, and patients in the hospital. The flighting MARs controlled flighting according to the coordinate curve shown in Fig. 3.

Conclusions: MARs were useful in aiding doctors, nurses, and patients to improve medical treatments, healthcare, and security.

Acknowledgements: This work was supported by grants from National Natural Science Foundation of China (Grant No. 61673007, 61271114, 61203325, and 81072006) and Innovation Program of Shanghai Municipal Education Commission (No. 14ZZ068).

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Fig. 1. Path planning of MAR avoiding the obstacles.

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Fig. 2. Immune control of MAR.

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Fig. 3. Flighting coordinate curve of MAR.

094

Effect of Ta Particles on Cytotoxicity and Cytokines Secretion of Human Monocytes

Yang Y.J. , Hu C. , Xie C. , Shao L.Q.

Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou City, China

Aims: To explore the effect of tantalum metal panicles on cytotoxicity and cytokine secretion of human monocytes for further understanding of the prospect of tantalum metal as dental implant coating.

Methods: Negative control group (cell medium and THP-1 cell, without experimental material), positive control group (cell medium with THP-1 cell and LPS [300 ng/mL]) (set the culture plate alone, prevented to affect the experimental group), HA group (cell medium with THP-1 cell and HA [SAR=10:1]) and Ta particles group (cell medium with THP-1 cell and Ta particles [SAR=10:1]) were included. Fifteen samples were included in each group. Cells and material were co-cultured for 24 h, 48 h and 72 h. And the expression level of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF- α) were evaluated by quantitative real-time PCR.

Results: The expression level of IL-6 and TNF-α in the negative control group was significantly lower than in the positive control, HA and Ta particles group (< 0.05). The expression level of IL-6 and TNF-α in HA and Ta particles group was significantly lower than the positive control group (< 0.05). The IL-6 and TNF- α expression in each group showed rising trend with no significant difference at different time points (> 0.05).

Conclusions: The tantalum had no negative effects on the secretion of IL-6 and TNF- α in THP-l cell. Furthermore, it could partially decrease the harmful effect of some inflammatory cytokines on inflammation and bone absorption around implants. It provides evidence for the clinical use of Ta particles.

Acknowledgements: This work was supported by National Natural Science Foundation of China (51672122) and Medical Scientific Research Foundation of Guangdong Province, China (A2017539).

095

Investigation into the Rates and Pathology of Nodosity Thyroncus in Residents of Areas with Differing Iodine Levels

Yang L.X. , Mi N. , Li Z. , Cong X.J. , Xu H.Q.

Endocrinology Department of Qinghai People's Hospital, Xining, China

Aim: To analyze the rates and pathology of nodosity thyroncus in residents of areas with differing iodine levels.

Methods: 3212 adults over the age of 20, residing in Xining, Qinghai province and Yushu, Tibet, from 2014 to 2016, were screened for thyroid nodules. Of the 3212 residents, 2055 were positive for thyroid nodules, with 927 cases found in the Xining area, and 1157 cases found in the Yushu area. 332 cases with nodules >1 cm were evaluated by fine-needle aspiration biopsy. The levels of urinary iodine, serum TSH, FT4, TGAb, TPOAb, and HbA1c, and body mass index (BMI) were also measured.

Results: The rate of thyroid nodules in adult residents of the Xining area was related to age, gender, levels of urinary iodine, serum TSH, TGAb, and TPOAb, and BMI. The rate of thyroid nodules in adult residents of Yushu, Tibet was related to age, gender, urinary iodine levels, and BMI. There were significant differences in the median urinary iodine, and serum thyrotrophic hormone levels (TGAb and TRAb) between the two groups (< 0.05). 11 cases were found to be malignant neoplasm of the thyroid gland in the Xining area; however no cases were detected in Yushu, Tibet.

Conclusions: Long-term iodine salt intake in adult residents of iodine-deficient areas may be associated with AITD and the ratio of thyroid nodes. Thyroid nodes also had a higher correlation with BMI and thyrotrophic hormone levels.

Acknowledgements: This work was supported by a grant from The Qinghai Province Science and Technology Infrastructure Project (Grant No. 2014- SF-530).

096

A 3D-imaging Anatomy Technique and Application Study

Duan S.Y. , Huang Q.W. , Ye F.

Medical Imaging Department, Zhongshan Hospital of Xiamen University

Aims: Discuss the 3D-imaging technique of volume rendering with separating, fusing, opacifying and false-coloring (SFOF-VR), and evaluate its application in the head and neck.

Methods: SFOF-VR was performed on data of 60 subjects (including the normal, variations and/or lesions) from the head-neck CT angiography (CTA) examination. Comparisons were made between SFOF-VR and Routine-VR (R-VR) in showing the artery, bone and joint.

Results: Internal carotid artery (ICA), Circle of Willis (CW), Occipital (C0), atlantoaxial joint (AAJ) and vertebral artery (VA) were independently or jointly showed in the 3D-images from the SFOF-VR. In 60 subjects, 16 cases were normal, 26 cases had variations (10 cases in CW, 9 in AAJ and 7 in VA) and 18 cases had lesions (11 cases with arteriosclerosis/aneurysm and 7 with fracture/dislocation of AAJ). Statistical comparison showed that images of SFOF-VR were much clearer than those of R-VR in displaying the normal anatomy and abnormal changes (P < 0.05).

Conclusion: SFOF-VR is a good 3D-imaging anatomic method, which can clearly and directly show the anatomy of ICA, CW, VA, C0 and AAJ. It can provide elaborate anatomy for imaging diagnosis and surgical operation.

Acknowledgements: This work was supported by the project grants from National Natural Science Foundation (Grant No. 81071214 and No. 30870690), China; Natural Science Foundation of Fujian Province (Grant No. 2015J01535).

097

Classification of Normal Esophagus and Early Esophageal Disease Based on SVM and KNN Classifiers

Zhang S.X.1 , Yan C.B.1 , Murat H.1 , Yao J.2 , Pazilya Y.3

1College of Medical Engineering Technology, Xinjiang Medical University, Urumqi, China; 2Department of Radiology, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, China; 3College of Basic Medicine, Xinjiang Medical University, Urumqi, China

Aims: To acquire a strong classification capability of X-ray images of normal esophagus and early esophageal disease by means of SVM and KNN classifiers.

Methods: Features were extracted based on gray-gradient and gray-level co-occurrence matrices. Subsequently, the dimensionality of the features data set was reduced and new principal component matrices were obtained. Classifiers of the support vector machine and KNN on new feature matrices were then adopted. Finally, the depicted parameters of each classification model were evaluated.

Results: The total PCA score was >1, the cumulative contribution rate was >90%, and we extracted seven principal components in the PCA feature set. The accuracy rates of the KNN (Where K = 3, 30% of the sample size) and SVM classifiers in normal esophageal classification were 94.30% and 85.70%, respectively, and in early esophageal cancer classification were 80% and 91.40%, respectively. With respect to the accuracy of normal esophageal X-ray images with texture features, SVM was superior to KNN. However, using the KNN (Where K = 3, 30% of the sample size) classifier, the classification accuracy of early esophageal cancer X-ray images was better.

Conclusion: The SVM classifier had superior classification ability of normal esophageal images and the KNN classifier had superior classification ability of early esophageal cancer images. The results of this study can provide a valuable reference for radiologists in the diagnosis of esophageal cancer, particularly in the diagnosis of early esophageal cancer, laying the foundation for the development of a computer-aided diagnostic system for esophageal cancer.

Acknowledgements: This work was supported by project grant from The National Natural Science Foundation of China (Grant No. 81560294, 81460281, 81160182).

098

Investigation on the Immunomodulatory Activity of Sparassis crispaPolysaccharides in Cyclophosphamide-induced Immunosuppressed Mice

Qu Y.D.1 , Zhang Y. Z.1 , Wang C.Y.1 , Wang D.1,2

1School of Life Sciences, Jilin University, Changchun, China; 2Zhuhai College of Jilin University, Jilin University, Zhuhai, China

Aims: Sparassis crispa, an edible mushroom, has been reported to show strongly anti-tumor effects in mouse models. The present study aims to investigate the immunomodulatory activity of polysaccharides obtained from Sparassis crispa in cyclophosphamide (CTX)-induced immunosuppressed mice.

Methods: Polysaccharides were obtained from Sparassis crispa water extract (SCPS) through deproteinization, four-fold alcohol precipitation and dialysis. Eight-week male BALB/c mice were intraperitoneally injected with CTX for 3 days, following with 2-week administration with SCPS from 100 to 400 mg/kg. Delayed type hypersensitivity test, lymphocyte proliferation assay and cytotoxic activity assay of natural killer cells were applied to confirm the immunomodulatory activity of Sparassis crispa polysaccharides. Finally, the serum levels of immunoglobulin and various cytokines were detected via enzyme-linked immuno sorbent assay (ELISA) method.

Results: Compared to model mice, SCPS strongly reduced hind paw swelling and enhanced the proliferation of lymphocyte and the cytotoxicity of natural killer cells in CTX-injected mice. Furthermore, compared to CTX-induced immunosuppressed mice, SCPS strongly enhanced the serum levels of IgG, IgA, interleukin (IL)-2, IL-12 and interferon-α (IFN-α), and suppressed the serum levels of IL-10, tumor necrosis factor-α (TNF-α) and nuclear factor kappa B (NF-κB).

Conclusion: Our present data successfully confirmed that SCPS showed improving immune function in CTX-induced immunosuppressed mice, which may be related with the modulation on NF-κB signaling pathway.

Acknowledgments: This work was supported by the Science and Technology Key Project in Jilin Province of P.R. China (Grant No. 20160204029YY).

099

Correlation Between Hemodynamic Parameters and the Initiation and Progression of Vulnerable Plaques in the Coronary Artery

Liu Z.M.1 , Li Y.J.1 , Qi Y.P.1 , Wang Q.2

1College of Mechanical Engineering and Applied Electronics Technology, Beijing University of Technology, Beijing, China; 2Department of Cardiology, PLA General Hospital, Beijing, China

Aims: To evaluate the relationship between the degree of stenosis in the left anterior descending (LAD) branch and the fluid parameters of plaques using CT angiography (CTA) and computational fluid dynamics (CFD), which provides a theoretical basis to evaluate the formation and development of vulnerable plaques for clinical application.

Methods: Based on CT images, an aorta-coronary hemodynamic model was set up, assuming the blood was a laminar, incompressible, and Newtonian fluid. CFD was used to simulate the blood flow. Different degrees of stenosis (AS = 0%, 50%, 70%, and 80%), wall pressure (WP), time average wall pressure (TAWP), wall shear stress (WSS), time average wall shear stress (TAWSS), and oscillatory shear index (OSI) were considered in the evaluation of the relevance of fluid parameters and degree of stenosis.

Results: As the degree of stenosis increased, the TAWSS at the front zone of the stenosis also increased. A relatively lower TAWSS zone and a higher TAWP zone appeared in the area immediately downstream of the stenosis, and no obvious changes were found near to or in the plaque region. When the AS = 0%, the TAWP and OSI values were normal, while the TAWSS showed a sharp change in the axial direction of the anterior descending branch in the plaque region.

Conclusions: The TAWP and TAWSS had a certain correlation with the formation and development of coronary stenosis. The TAWSS and its abnormal changes are likely one of the significant causes of coronary stenosis. The relationship between the OSI and coronary stenosis remains to be confirmed.

Acknowledgements: This work was supported by a project grant from The Specialized Research Fund for the Doctoral Program of Higher Education (No. 20131103110025).

0100

The Antiviral Activity of Coptis Alkaloids on the Transmissible Gastroenteritis Virus In Vitro

Zheng H.1,2 , Yang L.1,2,# , Zhu M.X.1,2 , Zhang Y.F.1,2

1Chongqing Academy of Animal Science, Rongchang, Chongqing, China; 2Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing, China

Aims: To elucidate the effects of coptis alkaloids on the transmissible gastroenteritis virus (TGEV) in vitro.

Methods: Coptis alkaloids, containing 86.7% berberine hydrochloride (C20H17NO4), were extracted from Coptis chinensis. Swine testicular (ST) cells were used as a viral host. The coptis alkaloids were added prior to the addition of the virus, 1 h prior to the addition of the virus, and following the addition of the virus. 72-h post-inoculation, cell activity was tested using the CCK-8 method and cytokine secretion was evaluated by RT-PCR.

Results: The coptis alkaloids were more effective against the TGEV when they were added prior to the virus (< 0.05). RT-PCR results indicated that the coptis alkaloids significantly increased the mRNA expression of IFN-γ, IFNAR-1, IFNAR-2, and IRF-3 in ST cells irrespective of the addition time.

Conclusions: The coptis alkaloids promoted cell resistance to the viral infection to differing degrees depending on the time of addition, and may be suitable for use as an antiviral drug for the prevention and treatment of swine disease caused by the TGEV.

Acknowledgements: This work was supported by a project grant from Chongqing Basic Research Funding (Grant No. 16437).

#These authors contributed equally to this work.

0101

Counting Missing Pattern Analysis of Interictal iEEG for Epileptic Focus Localization

Zhu G.H.1 , Hunter J.1 , Zong F.R.2

1School of ITEE, The University of Queensland, Australia; 2Queensland Brain Institute, The University of Queensland, Australia

Aims: Traditional methods struggle to precisely localize the epileptogenic zone (EZ) and the non-epileptogenic zone (NEZ) during interictal state. To differentiate the EZ from NEZ, missing ordinal patterns extracted from intracranial electroencephalogram (iEEG) are analysed in the time domain.

Methods: Our approach employed Counting Missing Pattern (CMP) indices to analyse two channel iEEG signals from 7500 recordings. Hénon map chaos and logistic map chaos signals are used to evaluate the performance of CMP, respectively. A Support Vector Machine (SVM) is implemented to classify the CMP features between the EZ and the NEZ. In order to obtain the optimal delay lag for evaluating different CMP indices, statistical analyses and classifying accuracies are performed assigning embedded dimensions from three to nine.

Results: The results show that CMP indices with four embedded dimensions can classify Hénon map signals and logistic map time series effectively. The CMP indices from the EZ are significantly higher than those from the NEZ (= 0.01) when the time delay is larger than five and embedded dimension is six. The accuracy of identifying the EZ increases dramatically when both signal length and embedded dimensions are increased.

Conclusions: CMP indices with an optimal time lag, extracted from interictal iEEG can provide additional valuable information to improve the localization of epileptogenic zones in presurgical evaluation.

Acknowledgements: This work was supported in part by the UQ 2016 Philanthropic Grant for Early Career Engineering Researchers (Biomedical Engineering) (Grant No. PG005-2016).

0102

Protective Action of Dang Gui Shao Yao San Powder in a Diabetic Nephropathy Rat Model

Li X.B.1 , Ma B.X.1 , Liu H.L.2 , Zhao D.Y.1 , Liu X.H.1 , Zhao X.1 , Zhang Y.T.3 , Liu W.1 , Wang T.1 , Yang J.C.1 , Li Z.Z.4 , Liu L.C.5

1Henan University of Chinese Medicine, Zhengzhou, China; 2The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, China; 3Guangzhou University of Chinese Medicine, Guangzhou, China; 4The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; 5Chinese People's Liberation Army General Hospital, Beijing, China

Aims: To investigate the effects of Dang Gui Shao Yao San powder (DSS) on diabetic nephropathy (DN) in rats, and to elucidate its preliminary mechanism.

Methods: A diabetic nephropathy rat model was induced by intraperitoneal injection of streptozotocin. Wistar rats were randomly divided into a control group, a model group, a DSS group, and a losartan group. At the end of experiment, serum creatinine (Cr), blood urea nitrogen (BUN), 24-h urinary protein (24-h UPr) were evaluated, and renal tissues were morphologically examined by micrography. Arginine vasopressin (AVP) in the plasma and aquaporin-2 (AQP-2) in the urine were detected by ELISA, and AQP-2 in renal tissue was detected by Western blotting and PCR.

Results: The increase in glomerular diameter and mesangium accumulation in diabetic rats were reduced by DSS treatment. Cr, BUN, and 24-h UPr levels in the DSS group were significantly decreased compared with the model group. The levels of AVP in the plasma and AQP-2 in the urine were significantly decreased in the DSS group. DSS treatment also decreased the expression of AQP-2 in renal tissue at both the protein and mRNA levels.

Conclusion: DSS can ameliorate experimental DN. The mechanism may be related to DSS inhibiting renal AQP-2 expression and the level of plasma AVP.

Acknowledgments: This work was supported by project grants from The Key Scientific Research Projects of Henan Province Colleges and Universities (Grant No. 16A360010), The Science and Technology Project of Zhengzhou City (Grant No. 20150310), The Young Key Teachers of Colleges and Universities in Henan Province (Grant No. 2016GGJS-080).

0103

Niclosamide Inhibits Cell Proliferation and Induces Apoptosis in Hepatocellular Carcinoma Cells via the Upregulation of Bim and the Downregulation of Mcl-1 and Survivin

Wang C.Z.1 , Xu H.J.1 , Shi X.Q.1 , Zhou X.Q.2 , Liao M.M.1 , Yang M.Y.1 , Zhao J.F.1

1Key Laboratory of Nanobiological Technology of The Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China; 2Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China

Aims: To investigate the effect of niclosamide on proliferation and apoptosis in human hepatocellular carcinoma cell lines (HepG2, QGY-7703, and SMMC-7721) and to elucidate the underlying mechanism of action. Hepatocellular carcinoma (HCC) is the sixth most common malignant tumor worldwide, with a high mortality rate at advanced stages.

Methods: Cell proliferation and clonality were measured using MTT and colony formation assays, respectively. Assessment of apoptosis was performed by flow cytometry and Hoechst Staining. The mRNA and protein levels were analyzed by real-time PCR and Western blotting, respectively.

Results: Niclosamide suppressed the proliferation, inhibited clone formation, and induced apoptosis of hepatocellular carcinoma cells (HepG2, QGY-7703, and SMMC-7721) in a dose- and time-dependent manner. Niclosamide synergized with cisplatin to promote apoptosis of hepatocellular carcinoma cells. Following niclosamide treatment, the pro-apoptotic protein, Bim, was upregulated, while the anti-apoptotic proteins, Mcl-1 and Survivin, were downregulated, and phosphorylated Stat3 (Y705) was inactivated.

Conclusion: Niclosamide may prove to be a single or combined drug treatment for hepatocellular carcinoma, which acts via the Stat3 signaling pathway.

Acknowledgements: This work was supported by The National Natural Science Foundation of China (Grant No. 81302075, 81272193, and 81402001) and The Fundamental Research Funds for Central South University (Grant No. 2017zzts391).

0104

Adsorption of a Tumor-Associated Antigen onto Crystalized Rapamycin Nanoparticles for Combined Immuno- and Antitumor Therapy

Pan C.H.1,2 , Yen J.Y.1 , Hsiao Y.J.1

1National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Taiwan; 2Graduate Institute of Immunology, China Medical University, Taiwan

Aims: To evaluate rapamycin nanoparticles adsorbed with a tumor antigen (ovalbumin) as a combined vaccine to suppress tumor growth and enhance the immune response. Rapamycin (Sirolimus) is an mTOR inhibitor that suppresses tumor growth, low doses of which have been reported to enhance the immune response.

Methods: Rapamycin was crystalized into nanoparticles and analyzed using a particle analyzer. The protein binding capacity of the rapamycin particles, and their ability to inhibit cell proliferation were analyzed. The immune activation of the rapamycin particles was also tested both in vitro and in vivo.

Results: Rapamycin nanoparticles had a diameter around 500 nm, a zeta potential of +9 mV, and a protein binding capacity of 8 μg/mg. The rapamycin nanoparticles had a similar inhibitory effect on Raw264.7 macrophage cells as rapamycin. The mTOR phosphorylation was also reduced in a similar level by either rapamycin nanoparticles or rapamycin. In addition, the expression of CD80 and CD86 was not affected by the rapamycin nanoparticles, and immune responses were elicited by the rapamycin particulate vaccine.

Conclusions: The rapamycin particulate tumor vaccine has a potential to induce an immune response to a tumor antigen and inhibit tumor growth.

Acknowledgements: This work was supported by National Health Research Institutes (IV-105-PP-24).

Keywords: Rapamycin; Nanoparticle; Tumor vaccine; Immunotherapy

0105

Rapid Evaluation of Antioxidants using a Self-Assembled Capillary Electrophoresis-Chemiluminescence System

Pang Y.1 , Yang F.Q.2 , Gou Q.2 , Xia Z.N.2

1Department of Mechanical Engineering, Tsinghua University, Haidian District, Beijing, China; 2Department of Pharmaceutics, College of Chemistry and Chemical Engineering, University of Chongqing, Chongqing, P. R. China

Aims: To establish technology for the rapid evaluation of antioxidants, and to apply this technology to the quantification of the antioxidative activity of matrine and oxymatrine.

Methods: An online analysis system of capillary electrophoresis (CE) combined with chemiluminescence (CL) detection was self-assembled and employed to evaluate the two potential antioxidants. A constant CL signal was generated from the reaction of luminol with ·OH (using the H2O2-Cu2+ system). When the reaction was exposed to the potential antioxidants, their ability to quench ·OH led to the inhibition of CL peak intensity. Each quantitative decrease in CL corresponded to antioxidative potential. The antioxidative activity of matrine and oxymatrine against ·OH was investigated at different concentrations from 1 to 20 mmol/L.

Results: The fitting curves of the ·OH scavenging ratios towards different concentrations of matrine and oxymatrine were obtained and the curvilinear equations were as follows:

display math
display math

Based on these equations, the IC50 values for the ·OH quenching effects of matrine and oxymatrine were calculated to be 7.08 and 4.21 mmol/L, respectively.

Conclusions: The antioxidative activity of oxymatrine against ·OH was much stronger than that of matrine, based on the smaller IC50 value. The oxygen atoms in the molecular structure of oxymatrine likely promoted electronegativity, and consequently resulted in a higher antioxidative activity.

0106

Identification of CK2 Inhibitors from Natural Products Using Pharmacophore-Based Virtual Screening and Molecular Docking

Qi X.Q. , Zhang N. , Chen W. J. , Tang S. , Zhou Y.

College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China

Aims: To perform pharmacophore-based virtual screening and molecular docking of promising natural compounds as protein kinase CK2α inhibitors. CK2 is considered a valuable therapeutic target for cancer therapy, and the discovery of CK2 inhibitors with new scaffolds is of importance.

Methods: A dataset of 49 known CK2α inhibitors with diverse structures was selected to generate pharmacophore models using the HypoGen module of Discovery Studio 2.5. Subsequently, the well-validated pharmacophore model, Lipinski's rule of five, and ADMET property evaluation, were employed to screen the natural product database. Docking studies were performed to further validate the potential hits as CK2α inhibitors.

Results: The best pharmacophore model (Hypo1) with the highest correlation (0.887), the lowest RMSD (1.477), and the highest cost difference (70.057), included one hydrogen bond acceptor (HBA), one hydrogen bond donor (HBD), one hydrophobic (HY) and one ring aromatic (RA) feature. Docking results yielded two novel hit molecules with 2-propenone and coumarin scaffolds, which possessed higher Gold scores of 58.89 and 52.91, respectively, and displayed holistic recognition mechanisms with CK2α by simultaneously forming polar interactions with Val116 (hinge region) and Lys68 (positive area).

Conclusions: These findings provide a valuable strategy for rational screening of novel CK2 inhibitors. Derivatives of the two hits will be subjected to further synthesis and biological activity evaluation.

Acknowledgements: This work was supported by grants from The Beijing Municipal Commission of Education Research Projects (Grant No. KM201610005031) and The China Scholarship Council Awardee (Grant No. 201706545010).

0107

An Authenticity Survey of Herbal Medicines from Cynanchum Using DNA Barcoding

Guo M.Y. , Ren L. , Pang X.H.

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P.R. China

Aims: To survey the authenticity of commercial samples of three herbs from Cynanchum (Cynanchi Paniculati Radix et Rhizoma, Cynanchi Stauntonii Rhizoma et Radix, and Cynanchi Atrati Radix et Rhizoma) recorded in the Chinese Pharmacopoeia (2015 edition) using a DNA barcoding technique.

Methods: Crude drug samples of the three herbs were collected from herbal markets, hospitals, drug stores, and online shops in several provinces and municipalities in China. All the commercial samples were tested using a standard DNA barcoding database that was constructed on the basis of commonly used medicinal species of Cynanchum.

Results: No adulterants were found among the commercial samples of Cynanchi Paniculati Radix et Rhizoma and Cynanchi Stauntonii Rhizoma et Radix, while all the Cynanchi Atrati Radix et Rhizoma samples were authenticated as adulterants, indicating potential safety risks of medicine use. The three main adulterants of Cynanchi Atrati Radix et Rhizoma were C. glaucescens, C. mongolicum, and Ampelopsis japonica.

Conclusions: DNA barcoding is a useful tool to accurately identify raw crude drugs and to detect adulterants from Cynanchum, and it is an efficient technique to supervise commercial products on the medicine market, thus guaranteeing drug safety and protecting consumers’ interests.

Acknowledgements: This work was supported by grants from the National Natural Science Foundation of China (Grant No. 81573541) and CAMS Innovation Fund for Medical Sciences (Grant No. 2017-I2M-1-013).

0108

Forskolin Regulates Cdh23 Expression Via the PKA Signaling Pathway

Wang Z.M.1 , Zeng P.2 , Huang X.M.2 , Cheng X.L.2 , Wang A.P.2 , Dong J.H.2

1Department of Clinical Laboratory, Weifang City People's Hospital, Weifang, Shandong Province, China; 2Weifang Medical College, Weifang, Shandong Province, China

Aims: To investigate the effect of Forskolin on cdh23 expression, and to elucidate its regulatory mechanism with a view to discovering the target of Forskolin in Usher syndrome.

Methods: The mRNA expression of cdh23 in Gpr98tmIPwh/J mice was detected by quantitative reverse transcription PCR (qRT-PCR). A cdh23-promoter reporter gene construct was co-expressed with the VLGR1 receptor in HEK293 cells, and the effect of increasing concentrations of Forskolin (0 μM, 0.1 μM, 0.5 μM, 1 μM, 10 μM, 100 μM) and 10 μM H89 on the activity of cdh23 was observed.

Results: The mRNA expression of cdh23 in Gpr98tmIPwh/J mice was significantly lower than that in control mice (< 0.05). Forskolin significantly increased the activity of the cdh23 promoter in a time- and concentration-dependent manner. 10 μM Forskolin had the most obvious effect, with the difference being statistically significant (P < 0.05). 10 μM PKA inhibitor H89 significantly inhibited the activity of cdh23 (< 0.05). The mRNA expression of cdh23 was significantly increased following transfection of VLGR1 (< 0.05).

Conclusions: Forskolin mediated the expression of cdh23 via the cAMP-PKA pathway. The VLGR1 receptor-mediated signaling pathway may also play an important role in the regulation of cdh23 expression by Forskolin, but requires further study.

Acknowledgements: This work was supported by a project grant from The National Natural Science Foundation of China (Grant No. 81500798) and The Natural Science Foundation of Shandong Province (Grant No. ZR2014JL050).

0109

Genome-Wide Association Study of Alzheimer's Disease Using Tissue-Specific Network Analysis

Meng X.L.1,2 , Cong W.1 , Liang H.1 , Li J.1

1College of Automation, Harbin Engineering University, Harbin, China; 2Harbin Huade University, Harbin, China

Aims: To better understand the pathophysiology of Alzheimer's disease by constructing a brain-specific (BS) functional network using the CSF t-tau/ Aβ1-42 ratio. CSF is in direct contact with the brain, and thus, more closely reflects brain-associated biochemical events than any other biological fluid. Moreover, tissue context plays a key role in gene function.

Methods: We randomly divided the 843 ADNI participants into two groups: G1 (422) and G2 (421). Quality control (QC) was performed on G1 and G2 using PLINK v1.07. We carried out meta-analysis on gene-wise findings, and subsequently used the GIANT webserver to construct both an ‘all tissues’ and Brain-Specific (BS) network and enrichment analysis.

Results: We obtained a total of 563,980 SNPs and tested for association with the trait. We constructed both an ‘all tissues’ and BS network using 9 gene sets (APOC1, APOE, TOMM40, PVRL2, ASPDH, ASGR1, GRM2, FAM189A2, and RPA1) and pruned all low-probability (<0.5) edges.

Conclusions: Enrichment analysis of the BS network identified chylomicron remnant (= 8.48E-03), triglyceride-rich lipoprotein particle (= 5.65E-03), and very-low-density lipoprotein particle (= 1.06E-03) clearance as important biological events in Alzheimer's disease.

Acknowledgements: This work was supported by a grant from The National Key Scientific Instrument and Equipment Development Projects of China (Grant No. 2012YQ04014010) and The National Natural Science Foundation of China (61773134).

0110

Effects of the Aqueous Extract of Cornus Officinalis Fruits on the Serotonin, Norepinephrine, and Neuroendocrine Systems in Behavioral Despair Models of Mice

Wang J.M.1,2 , Zhang Y.Y.2 , Cui Y.1,2 , Feng W.S.1,2 , Gao J.J.2

1Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Chinese Medicine, Zhengzhou, China; 2College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China

Aims: To investigate the effects of the aqueous extract of C. officinalis fruits (WECOF), which are traditionally used in Eastern Asia as medicine, and to elucidate its underlying mechanisms.

Methods: WECOF was orally administrated to mice for 14 consecutive days at doses of 50, 100, and 200 mg/kg. The despair behaviors of the mice were observed using the forced swimming (FST), tail suspension (TST), and open field (OFT) tests, and by the analysis of intracerebral biochemical markers.

Results: All doses of WECOF significantly reduced the immobility time in the TST and the FST (< 0.05). However, no significant difference in behavior was observed in the OFT. Furthermore, the FST produced a noticeable decrease in the cerebral levels of serotonin and NE, and a marked elevation in the serum levels of corticotrophin releasing factor (CRF), adrenocorticotropic hormone (ACTH), and corticosterone (CORT). Treatment with WECOF (50, 100, and 200 mg/kg) elevated cerebral serotonin and NE, and decreased serum CRF, ACTH, and CORT levels.

Conclusions: WECOF appears to have an antidepressant effect in behavioral despair models of mice, and its underlying mechanisms may involve the serotonin, norepinephrine, and neuroendocrine systems.

Acknowledgements: This work was supported by project grants from The National Natural Science Foundation of China (Grant No. 81773928), The National Science & Technology Pillar Program of China during the Twelfth Five-Year Plan Period (Grant No. 2011BAI06B02), The Program for Science & Technology Innovation Talents in Universities of Henan Province (Grant No. 16HASTIT032), The Funding Scheme for Young Key Teachers of Colleges and Universities in Henan Province (Grant No. 2014GGJS-072), The Science and Technology Project of Zhengzhou (Grant No. 20150309), and The Graduate Innovation Fund Project in Henan University of Chinese Medicine (Grant No. 2013YCX001).

In The Context Of Abandonment Of The One-Child Policy, Lake Taihu Basin Health In East China

Li B.1, Pei Y.X.1, Ge R.S.2

1University of Jiaxing, Jiaxing, China; 2Wenzhou Medical University, Wenzhou, China

Objectives:

Concerning nowadays “Exterminating Level V- Water” campaign in Zhejiang province, as well as special ecological compensation fund containing a linkage with Jiangsu province, in the context of abandonment of the One-child policy in China from January 1st, 2016, it is necessary to check Lake Taihu watershed health.

Methods:

Literature research method and field survey method.

Results:

Taihu in the Yangtze Delta is the third largest lake in East China. Rapidly developing Lake Taihu basin is composed of three provinces and one municipality, namely Zhejiang, Jiangsu, Anhui and Shanghai, with an area of 36 900 square kilometers, and contributes above 10% total GDP of China while occupying about 4% of the whole land area. A massive bloom of cyanobacteria in Lake Taihu ever reached 700 million tons. According to “Comprehensive Plan of Lake Taihu (2012–2030)”, we analyzed basic watershed health profile of Lake Taihu for decision-maker. As one of social subsystem, population above 65 years accounted for above 20% of the total population in Lake Taihu basin. And Zhejiang province would control pollutants and solve waterborne issues even in the trans-boundary area of Lake Taihu basin through “Exterminating Level V- Water” campaign recently.

Conclusions:

There are a little mature evaluation of Lake Taihu health considering population policy and ecological compensation. Obviously, global warming may change some aspects of basin water circles and affect watershed health. We think that “Castle Peak Green Water is Jin-shan Silver” proposed in Zhejiang would be an ideal for Lake Taihu basin health and ecological service evaluation in China. Furthermore, in the context of abandonment of the One-child policy, “Exterminating Level V- Water” campaign would not only concern Zhejiang province, but also involve the whole Lake Taihu basin, highlighting that more attention should be directed towards basin-size input-output analysis.

Acknowledgements:

The authors acknowledge the Zhejiang Province for Key Innovation Team Project of Circular Economy and Development of Transformation & Upgrading (Grant: Zhe-Wei-Ban, [2012]68), Humanities and Social Sciences Planning Fund under Ministry of Education (14YJAZH030), South China Institute of Technology for program of International S&T Cooperation (Subproject, 2011DFA60290), Chongqing University for National Social Science Fund of China (Subproject, 12&ZD209), Jiaxing Environmental Protection Bureau (Grant: 00512086), Philosophy Social Sciences Foundation in Zhejiang province (14NDJC005Z), Zhejiang Federation of Humanities and Social Sciences Circles (2013N101).

IWHEM0026

Analysis On Sanitary Condition Of Rural Drinking Water In Xuchang

Liu J.J.1,2, He X.1, Shi D.2, Qin D.H.3, Lu H.J.1

1Institute of Poromechanics, Wuhan Polytechnic University, Wuhan, China; 2School of Geoscience and Technology, Southwest Petroleum University, Chengdu, China; 3School of Civil Engineering and Architecture, Southwest Petroleum University, Chengdu, China

Objectives:

To understand the sanitary condition of rural drinking water in Xuchang city of Henan province, and provide scientific basis to strengthen the sanitation management of drinking water.

Methods:

The water samples in high water period and low water period from 45 drinking waters safety project places were tested and analyzed accordance to relative national standards GB 5750–2006 and GB 5749–2006, respectively, from 2013 to 2015.

Results:

The qualified rate of Xuchang rural drinking water in 2013–2015 is 38.10%, 49.62% and 72.23% respectively. Although the qualified rate is rising, it is still unsatisfied. The main unqualified indexes are total number of colonies, total coliforms, heat-resistant coliform bacteria, manganese, chloride and fluoride. In two towns, the fluorine content of groundwater is severely overweight, exceeds 2 times of the standard.

Conclusions:

The qualified rate of rural drinking water was low; therefore, we should strengthen the management of rural water supply station to make sure the safety of drinking water. It is suggested that government increase investment, enhance training, strengthen supervision to ensure the hygiene quality of drinking water.

Acknowledgements:

Supported by the Natural Science Foundation of China under Grant No.51174170.

IWHEM0027

Numerical Modelling Of The Effect Of Hydraulic Capture Of Petroleum Contaminated Groundwater In Dongying City

Liu J.J.1,2, Wang R.1, He X.1, Lu H.J.1, Zhang C.C.1

1Institute of Poromechanics, Wuhan Polytechnic University, Wuhan, China; 2School of Geoscience and Technology, Southwest Petroleum University, Chengdu, China

Objectives:

The research objectives are to reveal the formation mechanism and the spatial-temporal distribution law of petroleum contaminated groundwater, and evaluate the effect of hydraulic capture technology.

Methods:

Field monitoring was used to investigate the formation process of petroleum contaminated groundwater. Finite element method was used to simulate the spatial and temporal distribution of the polluted water, and evaluate the effect of hydraulic capture technology.

Results:

The in-situ monitoring results shown that petroleum polluted groundwater system was formed by the joint action of hydrodynamic field and water chemical field, and the groundwater circulation system is the carrier. Hydrodynamic field plays a leading role in the formation of groundwater pollution system. Using the finite element software ANSYS, the time and space distribution of oil pollution groundwater time and space were simulated, and given out the concentration distribution before and after cutting off sources of pollutants. The simulation results shown that hydraulic capture technology cut off 89% of groundwater petroleum contamination, petroleum contaminants in groundwater may be removed out by the hydraulic capture technique.

Conclusions:

Identify hydrogeological conditions is a prerequisite for hydraulic capture engineering design and cut off the sources of pollution is the key to prevent and control groundwater petroleum contamination.

Acknowledgements:

Supported by the Natural Science Foundation of China under Grant No.51174170.

IWHEM0028

Numerical Simulation Of Gas And Leachate Two Phases Flow In Municipal Landfill Engineering

Liu J.J.1,2, He X.1, Qin D.H.3, Wang Y.J.2, Lu H.J.1

1Institute of Poromechanics, Wuhan Polytechnic University, Wuhan, China; 2School of Geoscience and Technology, Southwest Petroleum University, Chengdu, China; 3School of Civil Engineering and Architecture, Southwest Petroleum University, Chengdu, China

Objectives:

Landfill site are the final disposal place of municipal solid waste, and plays a vital role to protect our living environment. However, the landfill also has a secondary pollution, the generated landfill gas and leachate are the most harmful substances. Estimating the gas and leachate output during landfill running is important to engineering design and ensure the landfill safety.

Methods:

According to the Buckley-Leveret multiphase percolation theory, based on the formation and transportation mechanisms of landfill gas and leachate, mathematical model of landfill gas and leachate two phases flow is established. Using the finite difference method software, the authors studied how the porosity, permeability, temperature, water content, and biogas production rate influence the landfill gas and leachate flow.

Results:

The mathematical model of landfill gas and leachate two phases flow was established, and through numerical simulation, the influence mechanisms of porosity, permeability, temperature, water content, and biogas production rate were got. The results showed that the landfill gas production increases with temperature and organic content. The high porosity and water content are helpful for landfill gas generation and migration. The landfill gas is generated after landfilled 3–5 months.

Conclusions:

Compaction degree, temperature, water and organic content are main factors which influence the generation and migration of landfill gas and water.

Acknowledgements:

Supported by the Natural Science Foundation of China under Grant No.51174170.

IWHEM0029

Numerical Simulation Of Groundwater Pollution Induced By Oil Tank Leakage

Liu J.J.1,2, He X.1, Qin D.H.3, Wang Y.J.2, Zheng Y.X.2

1Institute of Poromechanics, Wuhan Polytechnic University, Wuhan, China; 2School of Geoscience and Technology, Southwest Petroleum University, Chengdu, China; 3School of Civil Engineering and Architecture, Southwest Petroleum University, Chengdu, China

Objectives:

There is a certain level of leakage from oil tanks or other equipment of oil extraction plant or refinery, which causes groundwater or soil polluted. Numerical simulation of underground oil pollution is of great significance to understand groundwater pollution, and evaluate the recovery value of subsurface oil in refinery.

Methods:

Based on the percolation mechanics and diffusion theory, mathematical model of oil polluted groundwater flow was established, and gave out its numerical solution method. Using the developed FEM simulation program, the author simulated an actual oil tank leakage case in Nanchong city, and analyzed the simulation results.

Results:

The mathematical model of oil polluted groundwater movement includes two parts, one is groundwater flow model, the other is solute transport model. Galerkin FEM and iterative methods can solve the coupled model effectively. The numerical simulation shown that although the tank is relatively small, but because of the high velocity flow of groundwater and the diffusion effect of oil pollutant, the oil pollution area reached 210 m away, and infiltrate into underground aquifer after 1-year leakage. The soil permeability and groundwater flow velocity are the two most important factors which determining the scope and concentration of oil pollutant.

Conclusions:

FEM numerical simulation is an effective method to predict the oil pollutant movement. Before refinery design, it is essential to clarify the local hydrogeological conditions like soil permeability, porosity, groundwater flow velocity, etc.

Acknowledgements:

Supported by the Natural Science Foundation of China under Grant No.50874082.

IWHEM0040

The Effects of Motion Sensing Games for Training Children with Autism

Xu Y., Ji L.Z.

College of Education & Technology, Zhejiang University of Technology, Hangzhou, China

Objective:

To explore the effects of Motion Sensing Games to improve social skills, motor skills and daily living skills in children with autism.

Methods:

A randomized controlled experiment was conducted and participants in the experimental group received a thirty-minute training using motion sensing games named “Game System for Rehabilitation (V1.0)” once a week for 8 weeks. The Pediatric Evaluation of Disability Inventory (PEDI) was administered to assess the children in both groups before and after the experiment.

Results:

It showed interaction effect between groups and pre-post tests on all three dimensions of social skills (F(1,58) = 23.66, P < 0.01), motor skills (F(1,58) = 31.18, < 0.01) and daily living skills (F(1,58) = 102.77, < 0.01). Analysis of simple effects showed significant higher posttest scores than pretest scores in experimental group, but the controlled group at three dimensions of social skills (F(1,58) = 2.28, = 0.14), motor skills (F(1,58) = 2.06, = 0.16) and daily living skills (F(1,58) = 1.39, = 0.16) had no significant difference.

Conclusion:

This study supported that Motion Sensing Games had positive effects on children with autism.

Acknowledgements:

Supported by a project grant from the National Social Science Fund of China (Grand No.12&ZD229).

IWHEM0041

The Preliminary Development of Assessment Scales of Children with Autism Spectrum Disorder

Xu Y., Yang M.Y., Yao J.

College of Education & Technology, Zhejiang University of Technology, Hangzhou, China

Objectives:

To develop assessment scales for children with autism spectrum disorder to help autistic children early find, early diagnosis and early treatment.

Methods:

The framework of assessment scales was based on core symptoms of autism. The exploratory factor analysis was conducted for preliminary questionnaires, and obtains three subscales (social interaction, communication, repetitive behaviors), include total of 50 assessment items in the formal scale.

Results:

On the reliability, Cronbach's Alpha coefficient of subscales is 0.850-0.893. By using split-half method, the coefficient of subscales is 0.828-0.901. On construct validity, the correlation coefficient of subscales scores, subscales and scale scores were 0.628– 0.902. On criterion validity, the subscales scores between autistic children and normal children have significant differences. The critical of scale is 164.

Conclusion:

The assessment scale has relative ideal validity and reliability, which meets the basic requirements of scale development and can be used as assessment for autistic children.

Acknowledgements:

Supported by a project grant from the National Social Science Fund of China (Grand No.12&ZD229).

IWHEM0043

Transport of Cd(II) Through Clay Liners Containing Activated Fly Ash

He X., Xing B.H., Liu J.J., Qin X.T.

School of Civil Engineering and Architecture, Wuhan Polytechnic University, Wuhan, China

Objectives:

Clay plays an important role as technical landfill barriers to prevent the breakthrough of hazardous contaminants like heavy metals. However, many heavy metal ions can pollute groundwater by diffusion through the clay liners. Hence, there is a crucial need for improving the clay liner. In this paper, to assess the potential uses of clay containing activated fly ash as a landfill barrier material.

Methods:

To investigate the influence of activated fly ash on clay liners, four cases were considered by setting clay /fly ash at 0%, 3%, 5% and 7%. Through permeability, batch adsorption and column tests, the seepage properties, the Cd(II) adsorption capacity and transport parameters of the soils were performed.

Results:

The results have shown that the hydraulic conductivities of all soil specimens were in the range of 1.2–2.6 × 10−8 cm/s. The Cd(II) adsorption of improved clay was much larger than adsorption by the raw clay. This coefficient for the clay or the improved clay was 2.7 × 10−10 to 8.6 × 10−10 m2/s.

Conclusions:

The improved clay may be used as a good barrier material to attenuate contamination of Cd(II) in landfills.

Acknowledgements:

Supported by the Natural Science Foundation of China under Grant No.A020405, Grant No.11472292 and Grant No.51279089.

IWHEM0045

Study On The Treatment Process Of Slaughterhouse Wastewater By Anaerobic-Aerobic Method

Pei G.H.1, Wang Z.M.1, He X.2, Shi D.3Liu J.J.2,3

1School of Civil Engineering and Architecture, Southwest Petroleum University, Chengdu, China; 2School of Civil Engineering and Architecture, Wuhan Polytechnic University, Wuhan, China; 3School of Geoscience and Technology, Southwest Petroleum University, Chengdu, China

Objectives:

Slaughter wastewater mainly contains blood, oil, minced meat, feathers and manure, and belongs to high concentration organic wastewater. This paper presents a new anaerobic-aerobic treatment method of slaughter wastewater, and proved its effectivity through a practical engineering application example.

Methods:

According to the slaughter wastewater's characteristics of high amounts of suspended matter and high concentration of organic pollutants, based on the anaerobic-aerobic biological method, a new water treatment process was put forward. By compared the pre and post water quality, the key indicators like BOD5, CODcr, SS were analyzed to test the treatment results.

Results:

The laboratory test results of the treated water showed that this process has better effect. The CODcr decreased from 2000–3000 mg/l to 100 mg/l, BOD5 decreased from 1500–1800 mg/l 30 mg/l, and SS decreased from 300 – 800 mg/l to 70 mg/l. The treated water quality reached the wastewater discharge class A of Liaoning Province. Technical and economic analysis showed that the cost of this treatment process is 0.98 Yuan/m3.

Conclusions:

The combined anaerobic-aerobic biological treatment method is effective for slaughterhouse wastewater, and the economic cost is low. The treated water quality completely meets the requirement of local government.

Acknowledgements:

Supported by the China National Science and Technology Major Project under Grant No.2016ZX05013001.

IWHEM0046

Numerical Simulation Of The Air Pollution In A Gas Leakage Building

He X.1, Song H.R.1, Liu J.J.1Leng X.L.2, Liu J.S.1

1School of Civil Engineering and Architecture, Wuhan Polytechnic University, Wuhan, China; 2Institute of Rock and Soil Mechanics, Chinese Academy of Science, Wuhan, China

Objectives:

Building complexes are widely used in modern cities. Due to the complex space of the building, the air pollution rises quickly when gas leakage. On the other hand, the ventilation in the complex building is relatively insufficient, toxic gas concentration will increase rapidly and the air inside is seriously polluted.Exposure to high concentration of toxic gas and polluted air will do great harm to the heath of the peoples in the building, so it is important to make clear the generation and distribution of toxic gas when gas leakage.

Methods:

A numerical simulation research is carried out to reveal the toxic gas generation and distribution in different fire conditions.Basing on experiment results, a mathematical model is established to simulate the generation of toxic gas, and then, a three-dimensional mathematical model based on the theory of turbulent combustion. A finite element method is presented to solve the presented mathematical model and simulate the movement of the toxic gas and its pollution distribution.

Results:

Simulations show that the movement of smoke plume influenced by both buoyancy and forced convection can be divided into three special sections, which are 3D spiral vortex flow section, 2D stratified flow section and 1D longitudinal spreading section. When the speed of ventilation is less than 0.86 m/s,toxic gas concentration stratification in remote place is more clear than near the fire place,and toxic gas is mainly concentrated in the higher region near the ceiling; when the speed is higher than 0.86 m/s, the smoke is turbulent flow, toxic gas concentration area is not obvious.It is also found the maximum of toxic gas concentration decrease along the building, and the maximum concentration has a delay time along the building.

Conclusions:

Because of the gas leakage, the movement of the smoke is complex turbulent flow. When ventilation speed is less than 0.86 m/s, there is a high t toxic gas concentration area near the ceiling. To release the toxic gas concentration, adequate ventilation must be provided and the ventilation speed must be higher than 0.86 m/s.

Acknowledgements:

Supported by the Natural Science Foundation of China under Grant No. A020405 and Grant No.11472292.

IWHEM0051

A Mathematical Model For Health Risk Assessment Of Groundwater Contamination In Xingyang City

He X.1, Song H.R.1, Leng X.L.2Liu J.J.1, Liu J.S.1

1School of Civil Engineering and Architecture, Wuhan Polytechnic University, Wuhan, China; 2Institute of Rock and Soil Mechanics, Chinese Academy of Science, Wuhan, China

Objectives:

Health risk assessment is an important method to quantify the relationship between pollution and human health. Based on the way of physical intakes and mechanism of contaminants, the relationship between contaminants exposure dose and physical health response, a mathematical model is established to assess the health risk of groundwater contamination in Xingyang city.

Methods:

The study includes 5 steps, which are data collection,dose-response assessment,exposure assessment, risk characteristics and mathematical model establishment.Data of groundwater pollution and health conditions in Xingyang city are collected, then10 assessment modes of exposure dose computation and 2 modes of risk characteristics computation are adopted, and finally the mathematical model for health risk assessment of groundwater contamination in Xingyang city is established.

Results:

The assessment result indicated that groundwater in Xingyang city has been mainly contaminated by trichloroethylene,the carcinogenicity risk is much higher than normal standard and non-carcinogenicity risk is less than 1.0.Compared with the bar diagram of non- carcinogenicity risk, breathing air and drinking exposure are the main factors of carcinogenicity risk, the carcinogenicity risk caused by touching water and air exposure could be ignored.

Conclusions:

Health risk assessment is an important method to quantify the relationship between pollution and human health and assessment can be done in 5 steps, which are data collection,dose-response assessment,exposure assessment, risk characteristics and mathematical model establishment.The assessment result by the method proposed reveals trichloroethylene in the groundwater is main risk factor of the heath in Xingyang city.

Acknowledgements:

Supported by the Natural Science Foundation of China under Grant No. A020405 and Grant No.11472292, and the Natural Science Project of Hubei Province under Grant No.2015CFB426.

IWHEM0052

Research on the Optimization Strategies of Chinese Rural Living Environment

Hu C.B.1,2, Li F.Y.1, Wang J.T.2, Ding Y.F.1,2

1Marxism Research College, Bohai University, Jinzhou, China; 2School of Economics & Management, Shenyang University of Chemical Technology, Shenyang, China

Objectives:

Rural living environment is the main indicator of the standard of living of farmers. Improve rural living environment quality of construction, coordination between rural settlements and society, economy, resources and environment, effectively improve the rural landscape and the lives of farmers, promoting the socialist new rural construction.

Methods:

By Chinese rural living environment situation analysis and dilemma analysis, to explore effective way to improve rural living environment, thereby improving rural living environment theory, provide a reference for promoting the coordinated development of Chinese urban and rural and Chinese new rural construction.

Results:

Improvement and improvement of rural living environment is a long-term task of building a new socialist countryside. Improve rural living environment, need to be based on realistic conditions to narrow the difference between urban and rural areas, adaptation of the rural population and the number of settlements decreasing trend, improvement of rural resources, optimize the layout of the village, the village to promote intensive development.

Conclusions:

Optimization of rural living environment problem is a systematic project, we need a new perspective to look at, think, solve rural problems of human settlements. By rural people living environment, strengthening rural infrastructure and public services, and further improve the rural living environment, create a new socialist countryside, to promote the comprehensive development of China's rural areas.

Acknowledgements:

Supported by 2015 Shenyang Social Science Subjects (Grand No.SYSK2015-10-01).

IWHEM0059

An Improved Algorithm To Enhance Tonal Information In Cochlear Implant

Wang J.1, Zhang W.F.1, Wei Z.K.2Guan T.2

1School of Electronics and Communication, Shenzhen Institute of Information Technology, Shenzhen, Guangdong, China; 2Research Center of Biomedical Engineering, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong, China

Objectives:

An improved algorithm to enhance tonal information in cochlear implant was proposed for Mandarin Chinese speech recognition.

Methods:

Four frequency bands were used: 300-621, 621-1285, 1285-2657, and 2657-5499 Hz. Temporal envelope and periodicity cues (TEPCs) below 400 Hz were extracted by full wave rectification and low-pass filtering. TEPC in each band was multiplied by a sinusoid, the frequency of which was the harmonic frequency of F0 most close to the center frequency of each band. Signals from each band were combined together to obtain an output speech. Mandarin tone, word, and sentence recognition in quiet listening conditions were conducted for traditional continuous interleaved sampling (CIS) strategy and the improved algorithm.

Results:

The improved algorithm performed consistently better than CIS algorithm in Mandarin tone, word, and sentence recognition. Moreover, Sentence recognition rate was higher than word recognition rate, because of the important role played by contextual information. Finally, performances were better for tone 3 and 4 than those for tone 1 and 2, due to the easily identified features of tone 3 and 4.

Conclusions:

The improved algorithm based on F0-harmonics could enhance tonal information in cochlear implant speech processing. Further study will focus on verifying the effectiveness of the algorithm in noisy environment.

Acknowledgements:

This work was supported by the National Natural Science Foundation of China (Grants No. 81401539, 31271056), Shenzhen Municipal Science and Technology Program (Grant No. JCYJ20160527101807403), and Shenzhen Medical Engineering Laboratory for Human Auditory-Equilibrium Function.

IWHEM0061

Application Of Customer Relationship Management In Hospital Rehabilitation Nursing

Yang R.J.1, Guo L.1, Ge Y.H.2

1Hospital of Wuhan Polytechnic University, Wuhan, China; 2Business School, University of Shanghai for Science & Technology, Shanghai, China

Objectives:

To implement the “customer centered” management concept, the customer relationship management platform is established. Based on this platform, the hospital rehabilitation nursing is managed effectively to provide more favorable health protection for patients.

Methods:

The service concept of the single service object in traditional medical model is updated. The overall quality of medical staff is improved. The effective rehabilitation nursing plan and evaluation system are developed by realizing the remote visit and nursing system. Moreover, the comprehensive evaluation plan of nursing quality has been developed too.

Results:

The quality of nursing care is significantly improved. The patient's recovery rate is increased by more than 30%, and the incidence of medical disputes is decreased by almost 80%. Thus, hospital management costs are decreased by almost 50%.

Conclusions:

The implementation of modern management method can effectively improve the quality of hospital rehabilitation nursing, and reduce the cost of rehabilitation care. Thus, it has a good promotion effect.

LY001

Application of Lower Limbs Centrifugal Training to the Rehabilitation of Basketball Special Students’ Knee Joint Injury

Wang W.

Wuhan Sports University School Of Sport Science&Technology, Hubei, China

Aims:

To explore the effect of application of lower limbs centrifugal training to the rehabilitation of basketball special students’ knee joint injury.

Methods:

Selecting 150 basketball special students with knee joint injury from July 2015 to December 2015 as research objects (a total of 246 injured knees), using isokinetic muscle test and training system for lower limbs centrifugal muscle strength training, after the course of treatment for 1 month, comparing the total work and visual analog scale (VAS) of knee pain degree when the 246 injured knees were under isokinetic exercise with 60°/s, 120°/s and 180°/s respectively before and after the training.

Results:

After one month of training, when flexor and extensor were under the condition of 60°/s (low speed), 120°/s (intermediate speed) and 180°/s (high speed), the total work of the knee joint was always significantly higher than that before training (t = 21.730, 15.900, 27.260, 13.303, 13.072, 17.344, P < 0.001); when it was at the rotate speed of 60°/s, 120°/s and 180°/s, knee joint VAS points was significantly lower than that before training (t = 2.011, 11.980, 27.260, P < 0.05,P < 0.001).

Conclusions:

Lower limbs centrifugal training is helpful to protect the knee joint function of basketball special students who are susceptible to knee joint injury and reduce the pain degree.

LY002

Effects Of Adopting Exercise Intervention For Adolescents With Simple Obesity On Heart Rate, Blood Pressure And Lipid Metabolism

Zhang B.

Physical Educational College of Zhengzhou University, Henan, China

Aims:

To explore the effects of exercise intervention for adolescents with simple obesity on heart rate, blood pressure and lipid metabolism.

Methods:

25 cases of adolescents with simple obesity who participated into the summer exercise weight loss camp from July to August in 2015 were selected as study objects. Exercise intervention was given to them for 6 weeks, to measure the change of the resting heart rate and blood pressure before and after the intervention. The ultrasound diagnostic technology was taken to detect the flow mediate dilation (FMD) function of endothelium-dependent flow-mediated dilation, to gather the levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterin (LDL-C).

Results:

After 6 weeks’ exercise intervention, the resting heart rate, systolic pressure and diastolic pressure of adolescents with simple obesity are significantly lower than before, and FMD is significantly higher than before (P < 0.05). Serum levels of TC, TG, LCL-C are significantly lower than before (P < 0.05).

Conclusions:

Aerobic exercise intervention is helpful to reduce the heart rate and blood pressure of adolescents with simple obesity, to promote the lipid metabolism and improve the cardiovascular function.

LY003

Effect of Aerobic Exercise on Cardiovascular System and Some Blood Indexes of Human Body

Song H.Z.

Physical Educational College of Zhengzhou University, Henan, China

Aims:

To investigate the effect of aerobic exercise on cardiovascular system function and some blood related indexes of human body.

Methods:

Selecting 100 cases of healthy elderly people from July 2014 to June 2015 as research objects, dividing them into observation group and control group, each with 50 cases, according to whether they do aerobic exercise regularly, measuring the two groups’ body mass, blood pressure, brachial-ankle pulse wave velocity (baPWV) and ankle brachial index (ABI), and drawing their blood to test red blood cell (RBC), mean corpuscular volume (MCV), hemoglobin (Hb), and blood glucose (BGS) level.

Results:

The body mass index, pulse pressure and baPWV in the observation group were significantly lower than that in the control group; ABI in the observation group was significantly higher than that in the control group (t = 2.211–7.627, P < 0.05); the count of RBC in the observation group was significantly higher than that in the control group; the level of BGS in the observation group was significantly lower than that in the control group (t = 3.301–4.908, P < 0.05).

Conclusions:

Regular and moderate aerobic exercise can reduce the body mass and vascular stiffness of the elderly, improve blood related indexes, and enhance the function of heart and blood vessels.

LY004

Effect of Aerobic Exercise on Sleep Quality of College Students

Song H.Z.

Physical Educational College of Zhengzhou University, Henan, China

Aims:

To explore the effect of aerobic exercise on sleep quality of college students.

Methods:

Selecting 82 college students from July 2015 to December 2015 as research objects, carrying out 4 weeks of aerobic exercise, and using Pittsburgh Sleep Quality Index (PSQI) to evaluate the sleep quality.

Results:

After 4 weeks of training, time to fall asleep, sleeping time, sleep quality, sleep efficiency, sleep disorder, daytime function and total points were significantly lower than that before training (t = 8.782, 2.529, 4.504, 2.525, 4.527, 3.907, 9.973, P < 0.05).

Conclusions:

Aerobic exercise of moderate intensity is helpful to improve the sleep quality of college students.

LY005

Effects of Aerobic Exercise on Physical Fitness and Life Quality of Patients Who Have Taken Maintenance Hemodialysis

Zha J.

Physical Educational College of Zhengzhou University, Henan, China

Aims:

To explore the effect of aerobic exercise on physical fitness and life quality of patients who have taken maintenance hemodialysis.

Methods:

136 cases of patients received and cured with maintenance hemodialysis in nephrology department from July 2013 to December 2014 were selected as the research objects, and were divided into observation group and control group randomly according to the time of admission and odd or even number, with 68 cases in each group. The control group used conventional nursing intervention of hemodialysis and the observation group combined it with aerobic exercise intervention. Indexes like maximum oxygen uptake, metabolic equivalent, exercise time and life quality of patients in two groups were compared.

Results:

After 12 weeks of intervention, maximum oxygen uptake, metabolic equivalent and exercise time of the observation group were significantly higher than those of the control group (t = 2.142–3.065, P < 0.05); scores of physical field and psychological field, social field and environment field were significantly higher than those of control group, the comparative differences of which were statistically significant (t = 8.848–11.349, P < 0.05).

Conclusions:

Aerobic exercise intervention can significantly enhance the physical fitness of patients who have taken maintenance hemodialysis and improve their life quality.

LY006

Study On The Relation Between The Muscle Injury After Exercise And Blood Creatine Kinase Activity Change

Zha J.

Physical Educational College of Zhengzhou University, Henan, China

Aims:

To explore the relation between the muscle injury after exercise and blood creatine kinase activity change.

Methods:

75 cases of high performance athletes from September 2014 to June 2015 were selected as study objects, to do high intensity exercise. They were randomly divided into three groups, 25 cases in each group. The observation group 1 conducted transcutanclus electrical acupoint stimulation 1 h before exercise, the observation group 2 conducted transcutanclus electrical acupoint stimulation 1 h after exercise, and the control group didn't conduct anything, to compare the blood creatine kinase (CK), lactic dehydrogenase (LDH), lactic acid (LA) and subjective ratings of athletes in three groups before and after the exercise.

Results:

CK, ADH, and LA of the observation group is significantly lower than that of control group (P < 0.05). CK, ADH, LA of the observation group 1 is significantly lower than that of the observation group 2 (P < 0.05). Subjective ratings of the observation group is significantly lower than that of the control group (P < 0.05), and the subjective ratings of the observation group 1 is significantly lower than that of the observation group 2 (P < 0.05).

Conclusions:

After exercise, the content of creatine kinase in the blood after the injury of skeletal muscle is significantly increased. Making pretreating before exercise can significantly reduce the creatine kinase and the levels of other exercise injury factors, to relieve the discomfort after exercise.

LY007

Study Of The Influence Of Negative Emotions On The Incidence Of Mental Disorder Among College Students

Chen H.Y.

Wuhan University of Technology, Hubei, China

Aims:

Exploring the influence of negative emotions on the incidence of mental illness among college students, so as to provide reference for the prevention of mental illness.

Methods:

46 undergraduate students entering Hubei Academy of Fine Arts from September 2015 to September 2016 and diagnosed with negative emotions were selected as negative emotion group. At the same time, 46 cases of students without negative emotions were randomly selected as the control group, and the results of psychological diseases and the conditions of anxiety and depression between the two groups were observed and compared.

Results:

(1) In the incidence of mental illness, the morbidity of psychological disease of negative emotion group (54.35%) was significantly higher than that of non-negative emotion group (15.22%) and the difference was statistically significant (χ2 = 15.5250, P = 0.0001); (2) In the results of SAS and SDS score, the scores of negative emotion group research subjects were significantly higher than those of non-negative emotion group, and the difference between groups was statistically significant (P all <0.05).

Conclusions:

For college students, the existence of negative emotions can significantly improve the incidence of mental illness, and some individualized active intervention measures and means should be taken based on the undergraduates with negative emotions.

LY008

Effect of Weight Reduction by Exercise on Blood Biochemical Indexes of Obese Adolescents

Mao Y.

Northwestern Polytechnical University, Shaanxi, China

Aims:

To probe into the effect of weight reduction by exercise on body composition and blood lipid of obese male adolescents.

Methods:

Selecting 28 obese adolescents who volunteer to participate in the weight reduction by exercise from June to December, 2015 as research objects, adopting the method of aerobic exercise combined with diet intervention, and launching 4 weeks of closed weight reduction by exercise, in order to measure the changes of blood biochemical indexes before and after weight reduction by exercise.

Results:

After 4 weeks of weight reduction by exercise, the body mass, body mass index (BMI), body fat and body fat rate were all significantly lower than that before the exercise (t = 4.031–5.330, P < 0.05). Total cholesterol (TC), triglyceride (TG), and low density lipoprotein cholesterol (LDL-C) in serum were significantly lower than that before the exercise (t = 2.130–2.699, P < 0.05).

Conclusions:

Weight reduction by exercise combined with diet intervention can effectively reduce the body mass of obese adolescents, improve their body composition, and promote blood lipid metabolism.

LY009

Application Value Of Reticulocyte Parameters In Chemotherapy Of Acute Leukemia

Lei L.

The Third Affiliated Hospital of Qiqihar Medical University, Heilongjiang, China

Objectives:

To study the clinical significance of reticulocyte parameters change before and after in chemotherapy in acute leukemia.

Methods:

Thirty – two chemotherapeutic patients suffered from acute leukemia were improved after chemotherapy. The percentage of reticulocyte (RET %), reticulocyte absolute value (RET#), low fluorescent reticulocyte percentage (LFR), middle fluorescent reticulocyte percentage (MRF), high fluorescent reticulocyte percentage (HFR),white blood cells (WBC) and absolute neutrophil count and immature reticulocyte fraction (IRF) were detected by using the Sysmex 2000 automatic blood cell analyzer.

Results:

IRF, MFR and HFR after chemotherapy in the patients with leukemia were always declined, reached the lowest on 7 days of chemotherapy, began to recover on 14 days after chemotherapy, and recovered to the level before chemotherapy on 21 days of chemotherapy. LFR began to increase on 7 days of chemotherapy, and began to decrease on 14 days of chemotherapy. WBC and RET# were always decreased on 7, 14 days of chemotherapy, and began to recover on 21 days of chemotherapy.

Conclusions:

IRF, MFR HFR, and LFR are the sensitive indicators reflecting the bone marrow recovery and are earlier than WBC and RET# by 7 days.

LY010

Cerebral Protection Of Dexmedetomidine In Patients With Traumatic Brain Injury

Zhang J.T., Zhang H.W.

The Third Affiliated Hospital of Qiqihar Medical University, Heilongjiang, China

Objectives:

To investigate the cerebral protection of dexmedetomidine in patients with traumatic brain injury.

Methods:

Forty-three patients with traumatic brain injury were operated under general anesthesia and randomly assigned into two groups. The patients in group D (22 cases) were infused dexmedetomidine 1 μg/kg in 10 minutes before anesthesia induction, which was followed by pumping dexmedetomidine 0.4 μg/kg/h until 72 h after surgery. The patients in group C (21 cases) were infused as the controls. The blood samples from artery and internal jugular vein bulb were taken before anesthesia induction (T0), at 1 h of operation (T1), at the end of operation (T2) and 72 h after operation (T3) for the blood gas analysis. Cerebral extraction ratio for oxygen (CERO2) and arteriovenous blood oxygen difference (Da-jvO2) were calculated. Serum micro RNA (miR) -20 and miR -195 detected as well.

Results:

The Da-jvO2 and CERO2 at T3 were higher in group D than those in group C [(41 ± 8) mL/L vs (39 ± 9) mL/L and (32 ± 6) mL/L vs (30 ± 5) mL/L] (P < 0.05). Serum levels of miR-20 and miR-195 at T3 were lower in group D than those in group (P < 0.05).

Conclusions:

Dexmedetomidine has cerebral protection effect and can be used safely and effectively for postoperative sedation in the patients with traumatic brain injury.

LY011

Clinical Efficacy Of Bosentan Of Babies With Congenital Heart Disease Associated Pulmonary Arterial Hypertension

Zhang X.Z.1, Wang Q.2, Zhang S.J.1, Liu S.1, Sun J.J.1, Li J.1Ming L.1

1Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; 2School of Medicine, Shihezi University, Xinjiang Uygur Autonomous Region, China

Objectives:

To investigate clinical efficacy of bosentan of babies with congenital heart disease associated pulmonary arterial hypertension (CHD-PAH).

Methods:

A total of 90 CHD-PAH infants were randomly divided into captopril group 45 cases and bosentan group 45 cases, another 45 healthy infants served as the control group. Treatment Endothelin-1 (ET-1) and mean pulmonary arterial pressure (mPAP) in all infants were measured by ELISA and echocardiography (UCG) before treatment, after 4 and 8 weeks. Exercise tolerance was evaluated by observing the time changes of feeding 60 ml milk for babies in the observation group.

Results:

ET-1 and mPAP were significantly higher in the observation group than in the control group (P < 0.05). However, those were significantly lower than in the control group after treatment. The feeding time of 60 mL milk decreased statistically 4 weeks after bosentan treatment.

Conclusions:

Bosentan can not only effectively decrease the mean pulmonary artery pressure of infants with congenital heart disease, but also improve their exercise tolerance. Bosentan is effective in the treatment of CHD-PAH infants.

LY012

Clinical Study of Serum Visfatin and Adiponectin Levels in Patients with Type-2 Diabetes Mellitus Complicating Hypertension

Li Y.D.

The Third Affiliated Hospital of Qiqihar Medical University, Heilongjiang, China

Objectives:

To explore the relationship of visfatin and adiponectin with type-2 diabetes mellitus complicating hypertension

Methods:

Forty cases of hypertension patients, 38 cases of type-2 diabetes mellitus patients, 40 cases of hypertension patient s with 2 type diabetes mellitus, and 40 cases of normal controls were enrolled in this study .The level of serum visfatin and adiponectin, blood pressure, fasting blood glucose, blood lipid, body mass index (BMI) were measured, and their correlation with visfatin and adiponectin was analyzed.

Results:

The visfatin levels in both type-2 diabetes mellitus patients and in hypertension patients were significantly higher than in the control subjects (P < 0.05), while the levels in hypertension patients with type-2 diabetes mellitus were significantly higher than in the former two groups .The levels of adiponectin in type-2 diabetes mellitus and in hypertension patients were apparently lower than in the control subjects (P < 0.05), while levels in hypertension patients with type 2 diabetes mellitus were apparently lower than in those former two.

Conclusions:

The occurrence and development of hypertension and type-2 diabetes mellitus might be highly significantly correlated with the increase in visfatin and the decrease in adiponectin .

Acknowledgements:

This project is sponsored by the Qiqihar, Heilongjiang Province, the municipal science and technology bureau directive project (NO. 2015L054).The relationship between the visceral fat content of the newly diagnosed type 2 diabetes and the glycosylated hemoglobin and insulin resistance.

LY013

Ultrasonic Imaging Analysis Of Diffuse Malignant Peritoneal Mesothelioma

Jiang M.

The third Affiliated Hospital of Qiqihar Medical University, Heilongjiang, China

Objectives:

To retrospectively analyze the ultrasonographic performance of 112 cases of diffuse malignant peritoneal mesothelioma, and the characteristics of ultrasound imaging.

Methods:

Ultrasounds imaging and clinical of 112 cases of diffuse malignant peritoneal mesothelioma confirmed by pathology in our hospital were analyzed.

Results:

In 112 patients, including 45 males and 67 females, the proportion of male and female was 1:1.5. The age of onset was 48–83 years old, means age (57.0 ± 6.8) years old. A total of 105 cases (93.8%) had asbestos exposure history. The success rate of puncture biopsy was 94.6%.ultrasonic manifestations of diffuse malignant peritoneal mesothelioma showed diffuse thickening of the omentum in 112 cases (100%), thickening of parietal peritoneum in 96 cases (85.7%) and thickening of mesentery in 61 cases (54.5%), and thickness of uneven, irregular border. Blood flow spectrum of thickening omentum showed the resistance index increased. Abdominal lymphadenopathy was seen in 29 cases (25.9%), bowel involvement in 57 cases (51.0%). Peritoneal biopsy puncture feeling was hard. The strips were full, bright appearance, transparent or gelatinous. There was medium to larger as cites in abdominal cavity.

Conclusions:

Ultrasound examination and puncture biopsy guided by ultrasound for peritoneal thickening area make the disease get a clear pathological diagnosis.

MHS0011

A Kind Of Array Pulse Pattern Analysis Method For Wearable Health Monitoring

Cui J.1, Tu L.P.2, Hu X.J.3, Huang J.B.1Zhang Z.F.1Xu J.T.1

1Basic Medicine College, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2Institute of Interdisciplinary Research Complex, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 3Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai, China

Background:

The demand for intelligent wearable health products is increased day by day. Pulse is an important content of cardiovascular function monitoring, so pulse analysis is effectively used for disease diagnosis by the traditional Chinese medicine. For the sake of the realization of multi-dimensional pulse health monitoring, the research has established a kind of array pulse pattern analysis method based on multipoint micro-sensor and used the method for pulse classification of the traditional Chinese medicine.

Methods:

The array micro-sensor of PPS company is applied to the collection of radial arterial pulse picture; the time-dependent array pulse picture through the interpolation algorithm; MATLAB tools are used to select the optimal method for the extraction, denoising, the optimal channel selection, the determination of the main wave and amplitude, the confirmation of predicrotic wave and dicrotic wave, the calculation value of the array pulse volume (APV). The Support Vector Machine (SVM) and Random Forest (RF) method are used for the classification of 360 cases of pulse picture.

Results:

SVM, RF algorithm and APV analysis method are applied to the judgment of the normal, wiry and slippery pulses (the three kinds of most common pulse condition of the traditional Chinese medicine, thus increasing the classification accuracy from 72.95% to 82.94%.

Conclusion:

A kind of pulse waveform analysis method based on the change of array pulse volume (APV) is established. The research provides a new method for the wearable health assessment technique based on pulse evaluation.

Acknowledgements:

Supported by project grant from National Natural Science Foundation of China (Grand No. 81373556), Shanghai Traditional Chinese Medicine Science and Technology Innovation Project (Grand No. ZYKC201602003),Shanghai University of Traditional Chinese Medicine Science and Technology Project (Grand No. 2016YSN02).

MHS0013

Predicting Coronary Heart Disease Causative Genes Based On Protein-Protein Interaction Network

Huang Y.L.1Li S.Z.1, Sajid A.2, Liu R.Q.1

1Information Center, Beijing University of Chinese Medicine, Beijing, China; 2Department of Computer Science, University of Education, Lahore, Pakistan

Background:

Coronary heart disease (CHD) is one of the common diseases with highest morbidity, the objective of this study is to predict the causative genes of coronary heart disease (CHD) to provide reference for the prevention and treatment of CHD.

Methods:

By analyzing the topological features in protein-protein interactions network based on nearest neighbor search,the study evaluated the possibilities of candidate genes to be CHD-causative genes.

Results:

The test shows that 26%and 90%target genes are at the top 5%and top 50%of the list prioritized.

Conclusion:

The method can identify CHD genes with high performance with other traditional methods.

Acknowledgements:

Supported by a project grant from Beijing University of Chinese Medicine (Grand No.2016-JYB-QNJSZX005), Youliang Huang and Shuzhen Li are corresponding authors.

MHS0015

Indicators of an Appropriate Sensor for Traditional Chinese Pulse Diagnosis

Hu X.J.1Dong J.2Xu J.T.3, Cui J.3, Tu L.P.3, Huang J.B.3, Cui L.T.3

1Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, China; 3Basic Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai, China

Background:

Pulse palpation is an importance aspect in Traditional Chinese Medicine (TCM). Pulse sensor is a critical to how to acquire the comprehensive and effective information from wrists. So, we mainly pay attention to the indicators of an appropriate sensor for Traditional Chinese Pulse Diagnosis (TCPD) which can simulate TCM physician's taking pulse process.

Methods:

Firstly we summarize the existing researches about the TCPD's sensors and relevant fields. The pulse wave from single point sensor, PPS array sensor with 3*4 points and flexible electronic-skin pulse sensor based on flexure micro/nano technology (MNANO), secondly, is collected and analyzed. Thirdly, the morphology variability of every pulse wave is computed by the approximate entropy (APEN) for formalizing the difference between multipoint pulse signals. Finally, In order to evaluate the quality of pulse signals from MNANO, we compute the similarity of wave from MNANO and PPS using cosine similarity..

Results:

In the morphology variability analysis, the absolute APEN of every pulse wave in array sensor is different. Especially No.8 point's APEN is bigger than others. That shows PPS array sensor contains more effective information. The cosine similarity of two average pulse cycles of PPS and MNANO is up to 0.95. It shows MNANO is a well consistency with PPS.

Conclusion:

Therefore, according to the TCPD theory and above analysis, a suitable sensor simulating the process of TCPD should contains nine indicators involving nine key features: contact, array, thin, synchronicity and consistency, Small sensing unit, flexible, Multi-axis force, Great anti-interference performance, Static pressure and dynamic pressure, Stabilization and repeatability.

Acknowledgements:

Supported by project grant from National Natural Science Foundation of China (Grand No. 81373556), Shanghai Traditional Chinese Medicine Science and Technology Innovation Project (Grand No. ZYKC201602003), Shanghai University of Traditional Chinese Medicine Science and Technology Project (Grand No. 2016YSN02).

MHS0020

Modeling of Compounded CMUTs for Medical Imaging and Therapy

Gao S., Li Y., Zhang P.Y.

School of Physics and Electronics, Henan University, Kaifeng, China

Aims:

This paper reports a compounded capacitive micromachined ultrasound transducer (CMUT), which has the potential of CMUTs for multi-frequency transducer applications and medical application. The compounded CMUT (CCMUT) has a cell with low and high-frequency structures.